Fluorescent in situ hybridization for assessing the proportion of cells with trisomy 4 in a patient with acute non-lymphoblastic leukemia
A case of acute non-lymphoblastic leukemia is described in which fluorescent in situ hybridization (FISH) helped to resolve initially conflicting conventional cytogenetic results. Identification and assessment of the proportion of cells exhibiting trisomy of chromosome 4 in the patient's bone m...
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| Veröffentlicht in: | Annals of clinical and laboratory science 1995-07, Vol.25 (4), p.330-335 |
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| container_title | Annals of clinical and laboratory science |
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| creator | MARK, H. F. L SIKOV, W SAFRAN, H KING, T. C GRIFFITH, R. C |
| description | A case of acute non-lymphoblastic leukemia is described in which fluorescent in situ hybridization (FISH) helped to resolve initially conflicting conventional cytogenetic results. Identification and assessment of the proportion of cells exhibiting trisomy of chromosome 4 in the patient's bone marrow were made using a probe which hybridizes to the centromeric region of chromosome 4. These FISH results were consistent with our retrospective GTG-banded analysis but differed from another study conducted elsewhere. Factors such as culture conditions and duration of culture which may have influenced the proportion of leukemic to nonleukemic cells are discussed. Fluorescent in situ hybridization is a powerful adjunct to conventional cytogenetic analysis and may prove to be a useful tool for monitoring minimal residual disease in this patient. |
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F. L ; SIKOV, W ; SAFRAN, H ; KING, T. C ; GRIFFITH, R. C</creator><creatorcontrib>MARK, H. F. L ; SIKOV, W ; SAFRAN, H ; KING, T. C ; GRIFFITH, R. C</creatorcontrib><description>A case of acute non-lymphoblastic leukemia is described in which fluorescent in situ hybridization (FISH) helped to resolve initially conflicting conventional cytogenetic results. Identification and assessment of the proportion of cells exhibiting trisomy of chromosome 4 in the patient's bone marrow were made using a probe which hybridizes to the centromeric region of chromosome 4. These FISH results were consistent with our retrospective GTG-banded analysis but differed from another study conducted elsewhere. Factors such as culture conditions and duration of culture which may have influenced the proportion of leukemic to nonleukemic cells are discussed. Fluorescent in situ hybridization is a powerful adjunct to conventional cytogenetic analysis and may prove to be a useful tool for monitoring minimal residual disease in this patient.</description><identifier>ISSN: 0091-7370</identifier><identifier>EISSN: 1550-8080</identifier><identifier>PMID: 7668816</identifier><identifier>CODEN: ACLSCP</identifier><language>eng</language><publisher>Philadelphia, PA: Institute for Clinical Science</publisher><subject>Adult ; Biological and medical sciences ; Bone Marrow ; Chromosomes, Human, Pair 4 ; Hematologic and hematopoietic diseases ; Humans ; In Situ Hybridization, Fluorescence ; Karyotyping ; Leukemia, Myeloid, Acute - genetics ; Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis ; Male ; Medical sciences ; Trisomy ; Tumor Cells, Cultured</subject><ispartof>Annals of clinical and laboratory science, 1995-07, Vol.25 (4), p.330-335</ispartof><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>309,310,314,780,784,789,790,23928,23929,25138</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3612193$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7668816$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MARK, H. F. L</creatorcontrib><creatorcontrib>SIKOV, W</creatorcontrib><creatorcontrib>SAFRAN, H</creatorcontrib><creatorcontrib>KING, T. C</creatorcontrib><creatorcontrib>GRIFFITH, R. C</creatorcontrib><title>Fluorescent in situ hybridization for assessing the proportion of cells with trisomy 4 in a patient with acute non-lymphoblastic leukemia</title><title>Annals of clinical and laboratory science</title><addtitle>Ann Clin Lab Sci</addtitle><description>A case of acute non-lymphoblastic leukemia is described in which fluorescent in situ hybridization (FISH) helped to resolve initially conflicting conventional cytogenetic results. Identification and assessment of the proportion of cells exhibiting trisomy of chromosome 4 in the patient's bone marrow were made using a probe which hybridizes to the centromeric region of chromosome 4. These FISH results were consistent with our retrospective GTG-banded analysis but differed from another study conducted elsewhere. Factors such as culture conditions and duration of culture which may have influenced the proportion of leukemic to nonleukemic cells are discussed. Fluorescent in situ hybridization is a powerful adjunct to conventional cytogenetic analysis and may prove to be a useful tool for monitoring minimal residual disease in this patient.</description><subject>Adult</subject><subject>Biological and medical sciences</subject><subject>Bone Marrow</subject><subject>Chromosomes, Human, Pair 4</subject><subject>Hematologic and hematopoietic diseases</subject><subject>Humans</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>Karyotyping</subject><subject>Leukemia, Myeloid, Acute - genetics</subject><subject>Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Trisomy</subject><subject>Tumor Cells, Cultured</subject><issn>0091-7370</issn><issn>1550-8080</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kMFKxDAURYMo4zj6CUIW4q6QtE3TLGVwVBhwo-vhNZPaaJvUvBSpf-Bf2xmLq7c49x6474QsuRAsKVnJTsmSMcUTmUl2Ti4Q3xlLVZ6zBVnIoihLXizJz6YdfDCojYvUOoo2DrQZq2D39hui9Y7WPlBANIjWvdHYGNoH3_twhL6m2rQt0i8bGxqDRd-NND-ogPaT4OA9MtBDNNR5l7Rj1ze-agGj1bQ1w4fpLFySsxpaNFfzXZHXzf3L-jHZPj88re-2ScNLFpMcTFUpqRSHFIQWACwvZCqUMgK0lLkWqTCZYCoDWfPK6H0FmrOiULquRZmtyO2fd1rxORiMu87iYQM44wfcSTl1cyam4PUcHKrO7Hd9sB2EcTf_buI3MwfU0NYBnLb4H8sKnnKVZb8cP3wC</recordid><startdate>199507</startdate><enddate>199507</enddate><creator>MARK, H. F. L</creator><creator>SIKOV, W</creator><creator>SAFRAN, H</creator><creator>KING, T. C</creator><creator>GRIFFITH, R. C</creator><general>Institute for Clinical Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>199507</creationdate><title>Fluorescent in situ hybridization for assessing the proportion of cells with trisomy 4 in a patient with acute non-lymphoblastic leukemia</title><author>MARK, H. F. L ; SIKOV, W ; SAFRAN, H ; KING, T. C ; GRIFFITH, R. 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L</creatorcontrib><creatorcontrib>SIKOV, W</creatorcontrib><creatorcontrib>SAFRAN, H</creatorcontrib><creatorcontrib>KING, T. C</creatorcontrib><creatorcontrib>GRIFFITH, R. C</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Annals of clinical and laboratory science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MARK, H. F. L</au><au>SIKOV, W</au><au>SAFRAN, H</au><au>KING, T. C</au><au>GRIFFITH, R. C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fluorescent in situ hybridization for assessing the proportion of cells with trisomy 4 in a patient with acute non-lymphoblastic leukemia</atitle><jtitle>Annals of clinical and laboratory science</jtitle><addtitle>Ann Clin Lab Sci</addtitle><date>1995-07</date><risdate>1995</risdate><volume>25</volume><issue>4</issue><spage>330</spage><epage>335</epage><pages>330-335</pages><issn>0091-7370</issn><eissn>1550-8080</eissn><coden>ACLSCP</coden><abstract>A case of acute non-lymphoblastic leukemia is described in which fluorescent in situ hybridization (FISH) helped to resolve initially conflicting conventional cytogenetic results. Identification and assessment of the proportion of cells exhibiting trisomy of chromosome 4 in the patient's bone marrow were made using a probe which hybridizes to the centromeric region of chromosome 4. These FISH results were consistent with our retrospective GTG-banded analysis but differed from another study conducted elsewhere. Factors such as culture conditions and duration of culture which may have influenced the proportion of leukemic to nonleukemic cells are discussed. Fluorescent in situ hybridization is a powerful adjunct to conventional cytogenetic analysis and may prove to be a useful tool for monitoring minimal residual disease in this patient.</abstract><cop>Philadelphia, PA</cop><pub>Institute for Clinical Science</pub><pmid>7668816</pmid><tpages>6</tpages></addata></record> |
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| identifier | ISSN: 0091-7370 |
| ispartof | Annals of clinical and laboratory science, 1995-07, Vol.25 (4), p.330-335 |
| issn | 0091-7370 1550-8080 |
| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Adult Biological and medical sciences Bone Marrow Chromosomes, Human, Pair 4 Hematologic and hematopoietic diseases Humans In Situ Hybridization, Fluorescence Karyotyping Leukemia, Myeloid, Acute - genetics Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis Male Medical sciences Trisomy Tumor Cells, Cultured |
| title | Fluorescent in situ hybridization for assessing the proportion of cells with trisomy 4 in a patient with acute non-lymphoblastic leukemia |
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