Estradiol increases amounts of messenger ribonucleic acid for gonadotropin-releasing hormone receptors in sheep
Two experiments were contucted simultaneously to investigate the regulation of amounts of mRNA for GnRH receptors were measured before and after the preovulatory surge of LH following regression of the CL by prostaglandin F2 alpha (PGF2 alpha). So that the time of the preovulatory surge of LH could...
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| Veröffentlicht in: | Biology of reproduction 1995-07, Vol.53 (1), p.179-185 |
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| creator | HAMERNIK, D. L CLAY, C. M TURZILLO, A VAN KIRK, E. A MOSS, G. E |
| description | Two experiments were contucted simultaneously to investigate the regulation of amounts of mRNA for GnRH receptors were measured before and after the preovulatory surge of LH following regression of the CL by prostaglandin F2 alpha (PGF2 alpha). So that the time of the preovulatory surge of LH could be accurately predicted, ewes received two injections of PGF2 alpha on Day 14 of the estrous cycle. Anterior pituitary glands were collected from 5 control ewes on Day 14 of the estrous cycle (0 h after PGF2 alpha) and at 48, 72, and 96 h after PGF2 alpha (5 ewes per group). The second experiment was conducted to investigate the effects of 17 beta-estradiol on amounts of mRNA for GnRH receptors. On Day 14 of the estrous cycle, 20 ewes were ovariectomized (OVX); 15 of these ewes received estradiol implants when they were OVX (OVXEI). Sixteen hours after OVX, anterior pituitary glands were collected from 5 OVX and 5 OVXEI ewes, and the remaining OVXEI ewes received an i.m. injection of estradiol (25 micrograms in corn oil; OVXEI + E) to induce a preovulatory-like surge of LH. Anterior pituitary glands were collected from OVXEI + E ewes 18 or 54 h after injection of estradiol (n = 5 per group). Half of each anterior pituitary gland was used to measure the number of GnRH receptors. Poly(A)+ RNA was isolated from the remaining half of each anterior pituitary gland, applied to slot blots, and hybridized with a radioactive cDNA probe encoding the ovine GnRH receptor. Amounts of GnRH receptor mRNA were elevated (p < 0.05) approximately 2-fold 48 h after PGF2 alpha (i.e., prior to the onset of the preovulatory surge of LH) compared to 0, 72, or 96 h after PGF2 alpha. Acute (i.e., 16 h) removal of ovarian hormones did not influence amounts of mRNA for GnRH receptors (p > 0.05) compared to the amount on Day 14 of the estrous cycle. Administration of estradiol implants to OVX ewes for 16 h increased (p < 0.05) amounts of mRNA for GnRH receptors approximately 1.6-fold. |
| doi_str_mv | 10.1095/biolreprod53.1.179 |
| format | Article |
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L ; CLAY, C. M ; TURZILLO, A ; VAN KIRK, E. A ; MOSS, G. E</creator><creatorcontrib>HAMERNIK, D. L ; CLAY, C. M ; TURZILLO, A ; VAN KIRK, E. A ; MOSS, G. E ; University of Arizona, Tucson, AZ</creatorcontrib><description>Two experiments were contucted simultaneously to investigate the regulation of amounts of mRNA for GnRH receptors were measured before and after the preovulatory surge of LH following regression of the CL by prostaglandin F2 alpha (PGF2 alpha). So that the time of the preovulatory surge of LH could be accurately predicted, ewes received two injections of PGF2 alpha on Day 14 of the estrous cycle. Anterior pituitary glands were collected from 5 control ewes on Day 14 of the estrous cycle (0 h after PGF2 alpha) and at 48, 72, and 96 h after PGF2 alpha (5 ewes per group). The second experiment was conducted to investigate the effects of 17 beta-estradiol on amounts of mRNA for GnRH receptors. On Day 14 of the estrous cycle, 20 ewes were ovariectomized (OVX); 15 of these ewes received estradiol implants when they were OVX (OVXEI). Sixteen hours after OVX, anterior pituitary glands were collected from 5 OVX and 5 OVXEI ewes, and the remaining OVXEI ewes received an i.m. injection of estradiol (25 micrograms in corn oil; OVXEI + E) to induce a preovulatory-like surge of LH. Anterior pituitary glands were collected from OVXEI + E ewes 18 or 54 h after injection of estradiol (n = 5 per group). Half of each anterior pituitary gland was used to measure the number of GnRH receptors. Poly(A)+ RNA was isolated from the remaining half of each anterior pituitary gland, applied to slot blots, and hybridized with a radioactive cDNA probe encoding the ovine GnRH receptor. Amounts of GnRH receptor mRNA were elevated (p < 0.05) approximately 2-fold 48 h after PGF2 alpha (i.e., prior to the onset of the preovulatory surge of LH) compared to 0, 72, or 96 h after PGF2 alpha. Acute (i.e., 16 h) removal of ovarian hormones did not influence amounts of mRNA for GnRH receptors (p > 0.05) compared to the amount on Day 14 of the estrous cycle. Administration of estradiol implants to OVX ewes for 16 h increased (p < 0.05) amounts of mRNA for GnRH receptors approximately 1.6-fold.</description><identifier>ISSN: 0006-3363</identifier><identifier>EISSN: 1529-7268</identifier><identifier>DOI: 10.1095/biolreprod53.1.179</identifier><identifier>PMID: 7669847</identifier><identifier>CODEN: BIREBV</identifier><language>eng</language><publisher>Madison, WI: Society for the Study of Reproduction</publisher><subject>Animals ; arn mensajero ; arn messager ; Biological and medical sciences ; brebis ; ciclo estral ; cycle oestral ; Dinoprost - pharmacology ; Estradiol - pharmacology ; estrogenos ; ewes ; Female ; females ; femelle ; Fundamental and applied biological sciences. Psychology ; glandula pituitaria ; gonadoliberine ; gonadotrophine ; gonadotropin releasing hormone ; gonadotropinas ; gonadotropins ; hembra ; hormona liberadora de gonadotropina ; Hormone metabolism and regulation ; hormone receptors ; hypophyse ; Luteinizing Hormone - blood ; Luteinizing Hormone - metabolism ; Luteolysis - drug effects ; Mammalian female genital system ; messenger rna ; oestrogene ; oestrogens ; oestrous cycle ; ovariectomia ; ovariectomie ; Ovariectomy ; oveja ; ovulacion ; Ovulation ; pituitary gland ; Pituitary Gland, Anterior - drug effects ; Pituitary Gland, Anterior - metabolism ; prostaglandinas ; prostaglandine ; prostaglandins ; recepteur d' hormone ; receptores de hormonas ; Receptors, LHRH - genetics ; RNA, Messenger - metabolism ; Sheep ; Vertebrates: reproduction</subject><ispartof>Biology of reproduction, 1995-07, Vol.53 (1), p.179-185</ispartof><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3635180$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7669847$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>HAMERNIK, D. L</creatorcontrib><creatorcontrib>CLAY, C. M</creatorcontrib><creatorcontrib>TURZILLO, A</creatorcontrib><creatorcontrib>VAN KIRK, E. A</creatorcontrib><creatorcontrib>MOSS, G. E</creatorcontrib><creatorcontrib>University of Arizona, Tucson, AZ</creatorcontrib><title>Estradiol increases amounts of messenger ribonucleic acid for gonadotropin-releasing hormone receptors in sheep</title><title>Biology of reproduction</title><addtitle>Biol Reprod</addtitle><description>Two experiments were contucted simultaneously to investigate the regulation of amounts of mRNA for GnRH receptors were measured before and after the preovulatory surge of LH following regression of the CL by prostaglandin F2 alpha (PGF2 alpha). So that the time of the preovulatory surge of LH could be accurately predicted, ewes received two injections of PGF2 alpha on Day 14 of the estrous cycle. Anterior pituitary glands were collected from 5 control ewes on Day 14 of the estrous cycle (0 h after PGF2 alpha) and at 48, 72, and 96 h after PGF2 alpha (5 ewes per group). The second experiment was conducted to investigate the effects of 17 beta-estradiol on amounts of mRNA for GnRH receptors. On Day 14 of the estrous cycle, 20 ewes were ovariectomized (OVX); 15 of these ewes received estradiol implants when they were OVX (OVXEI). Sixteen hours after OVX, anterior pituitary glands were collected from 5 OVX and 5 OVXEI ewes, and the remaining OVXEI ewes received an i.m. injection of estradiol (25 micrograms in corn oil; OVXEI + E) to induce a preovulatory-like surge of LH. Anterior pituitary glands were collected from OVXEI + E ewes 18 or 54 h after injection of estradiol (n = 5 per group). Half of each anterior pituitary gland was used to measure the number of GnRH receptors. Poly(A)+ RNA was isolated from the remaining half of each anterior pituitary gland, applied to slot blots, and hybridized with a radioactive cDNA probe encoding the ovine GnRH receptor. Amounts of GnRH receptor mRNA were elevated (p < 0.05) approximately 2-fold 48 h after PGF2 alpha (i.e., prior to the onset of the preovulatory surge of LH) compared to 0, 72, or 96 h after PGF2 alpha. Acute (i.e., 16 h) removal of ovarian hormones did not influence amounts of mRNA for GnRH receptors (p > 0.05) compared to the amount on Day 14 of the estrous cycle. Administration of estradiol implants to OVX ewes for 16 h increased (p < 0.05) amounts of mRNA for GnRH receptors approximately 1.6-fold.</description><subject>Animals</subject><subject>arn mensajero</subject><subject>arn messager</subject><subject>Biological and medical sciences</subject><subject>brebis</subject><subject>ciclo estral</subject><subject>cycle oestral</subject><subject>Dinoprost - pharmacology</subject><subject>Estradiol - pharmacology</subject><subject>estrogenos</subject><subject>ewes</subject><subject>Female</subject><subject>females</subject><subject>femelle</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>glandula pituitaria</subject><subject>gonadoliberine</subject><subject>gonadotrophine</subject><subject>gonadotropin releasing hormone</subject><subject>gonadotropinas</subject><subject>gonadotropins</subject><subject>hembra</subject><subject>hormona liberadora de gonadotropina</subject><subject>Hormone metabolism and regulation</subject><subject>hormone receptors</subject><subject>hypophyse</subject><subject>Luteinizing Hormone - blood</subject><subject>Luteinizing Hormone - metabolism</subject><subject>Luteolysis - drug effects</subject><subject>Mammalian female genital system</subject><subject>messenger rna</subject><subject>oestrogene</subject><subject>oestrogens</subject><subject>oestrous cycle</subject><subject>ovariectomia</subject><subject>ovariectomie</subject><subject>Ovariectomy</subject><subject>oveja</subject><subject>ovulacion</subject><subject>Ovulation</subject><subject>pituitary gland</subject><subject>Pituitary Gland, Anterior - drug effects</subject><subject>Pituitary Gland, Anterior - metabolism</subject><subject>prostaglandinas</subject><subject>prostaglandine</subject><subject>prostaglandins</subject><subject>recepteur d' hormone</subject><subject>receptores de hormonas</subject><subject>Receptors, LHRH - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Sheep</subject><subject>Vertebrates: reproduction</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtv1DAUhS0EKtPCH0ACeUHZZfAjduJlVZWHVIkFdB3dca4zRo4d7EQj_j1GM2LL6i7Od77FPYS84WzPmVEfDz6FjEtOo5J7vuedeUZ2XAnTdEL3z8mOMaYbKbV8Sa5L-ckYb6WQV-Sq09r0bbcj6aGsGcYqoj7ajFCwUJjTFtdCk6MzloJxwkyzP6S42YDeUrB-pC5lOqUIY1pzWnxsMoba93Gix5TnFJFmtLisKZcqp-WIuLwiLxyEgq8v94Y8fXr4cf-lefz2-ev93WPjJDdrI1oYhbNoHOPWuEOnR2ZAG8l5p5RpDQjNdGuMGIVutUGGoHrDR2ASZO_kDflw9tbv_NqwrMPsi8UQIGLaytB1qvYZ_y_Ida-F7v6Cby_gdphxHJbsZ8i_h8sra_7-kkOxEFyGaH35h9URFO9ZxW7P2NFPx5PPOJQZQqhSOZxOJyUHPtQhK_fuzDlIA0y5qp6-c2N03ZBLJuUfrOicOg</recordid><startdate>19950701</startdate><enddate>19950701</enddate><creator>HAMERNIK, D. L</creator><creator>CLAY, C. M</creator><creator>TURZILLO, A</creator><creator>VAN KIRK, E. A</creator><creator>MOSS, G. E</creator><general>Society for the Study of Reproduction</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>19950701</creationdate><title>Estradiol increases amounts of messenger ribonucleic acid for gonadotropin-releasing hormone receptors in sheep</title><author>HAMERNIK, D. L ; CLAY, C. M ; TURZILLO, A ; VAN KIRK, E. A ; MOSS, G. E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f319t-24ad2fce9f01c9fb76d09a69311755949a26064992d26469e0ea5891da03a38f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Animals</topic><topic>arn mensajero</topic><topic>arn messager</topic><topic>Biological and medical sciences</topic><topic>brebis</topic><topic>ciclo estral</topic><topic>cycle oestral</topic><topic>Dinoprost - pharmacology</topic><topic>Estradiol - pharmacology</topic><topic>estrogenos</topic><topic>ewes</topic><topic>Female</topic><topic>females</topic><topic>femelle</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>glandula pituitaria</topic><topic>gonadoliberine</topic><topic>gonadotrophine</topic><topic>gonadotropin releasing hormone</topic><topic>gonadotropinas</topic><topic>gonadotropins</topic><topic>hembra</topic><topic>hormona liberadora de gonadotropina</topic><topic>Hormone metabolism and regulation</topic><topic>hormone receptors</topic><topic>hypophyse</topic><topic>Luteinizing Hormone - blood</topic><topic>Luteinizing Hormone - metabolism</topic><topic>Luteolysis - drug effects</topic><topic>Mammalian female genital system</topic><topic>messenger rna</topic><topic>oestrogene</topic><topic>oestrogens</topic><topic>oestrous cycle</topic><topic>ovariectomia</topic><topic>ovariectomie</topic><topic>Ovariectomy</topic><topic>oveja</topic><topic>ovulacion</topic><topic>Ovulation</topic><topic>pituitary gland</topic><topic>Pituitary Gland, Anterior - drug effects</topic><topic>Pituitary Gland, Anterior - metabolism</topic><topic>prostaglandinas</topic><topic>prostaglandine</topic><topic>prostaglandins</topic><topic>recepteur d' hormone</topic><topic>receptores de hormonas</topic><topic>Receptors, LHRH - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Sheep</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>HAMERNIK, D. L</creatorcontrib><creatorcontrib>CLAY, C. M</creatorcontrib><creatorcontrib>TURZILLO, A</creatorcontrib><creatorcontrib>VAN KIRK, E. A</creatorcontrib><creatorcontrib>MOSS, G. E</creatorcontrib><creatorcontrib>University of Arizona, Tucson, AZ</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>HAMERNIK, D. L</au><au>CLAY, C. M</au><au>TURZILLO, A</au><au>VAN KIRK, E. A</au><au>MOSS, G. E</au><aucorp>University of Arizona, Tucson, AZ</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Estradiol increases amounts of messenger ribonucleic acid for gonadotropin-releasing hormone receptors in sheep</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>1995-07-01</date><risdate>1995</risdate><volume>53</volume><issue>1</issue><spage>179</spage><epage>185</epage><pages>179-185</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><coden>BIREBV</coden><abstract>Two experiments were contucted simultaneously to investigate the regulation of amounts of mRNA for GnRH receptors were measured before and after the preovulatory surge of LH following regression of the CL by prostaglandin F2 alpha (PGF2 alpha). So that the time of the preovulatory surge of LH could be accurately predicted, ewes received two injections of PGF2 alpha on Day 14 of the estrous cycle. Anterior pituitary glands were collected from 5 control ewes on Day 14 of the estrous cycle (0 h after PGF2 alpha) and at 48, 72, and 96 h after PGF2 alpha (5 ewes per group). The second experiment was conducted to investigate the effects of 17 beta-estradiol on amounts of mRNA for GnRH receptors. On Day 14 of the estrous cycle, 20 ewes were ovariectomized (OVX); 15 of these ewes received estradiol implants when they were OVX (OVXEI). Sixteen hours after OVX, anterior pituitary glands were collected from 5 OVX and 5 OVXEI ewes, and the remaining OVXEI ewes received an i.m. injection of estradiol (25 micrograms in corn oil; OVXEI + E) to induce a preovulatory-like surge of LH. Anterior pituitary glands were collected from OVXEI + E ewes 18 or 54 h after injection of estradiol (n = 5 per group). Half of each anterior pituitary gland was used to measure the number of GnRH receptors. Poly(A)+ RNA was isolated from the remaining half of each anterior pituitary gland, applied to slot blots, and hybridized with a radioactive cDNA probe encoding the ovine GnRH receptor. Amounts of GnRH receptor mRNA were elevated (p < 0.05) approximately 2-fold 48 h after PGF2 alpha (i.e., prior to the onset of the preovulatory surge of LH) compared to 0, 72, or 96 h after PGF2 alpha. Acute (i.e., 16 h) removal of ovarian hormones did not influence amounts of mRNA for GnRH receptors (p > 0.05) compared to the amount on Day 14 of the estrous cycle. Administration of estradiol implants to OVX ewes for 16 h increased (p < 0.05) amounts of mRNA for GnRH receptors approximately 1.6-fold.</abstract><cop>Madison, WI</cop><pub>Society for the Study of Reproduction</pub><pmid>7669847</pmid><doi>10.1095/biolreprod53.1.179</doi><tpages>7</tpages></addata></record> |
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| ispartof | Biology of reproduction, 1995-07, Vol.53 (1), p.179-185 |
| issn | 0006-3363 1529-7268 |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Animals arn mensajero arn messager Biological and medical sciences brebis ciclo estral cycle oestral Dinoprost - pharmacology Estradiol - pharmacology estrogenos ewes Female females femelle Fundamental and applied biological sciences. Psychology glandula pituitaria gonadoliberine gonadotrophine gonadotropin releasing hormone gonadotropinas gonadotropins hembra hormona liberadora de gonadotropina Hormone metabolism and regulation hormone receptors hypophyse Luteinizing Hormone - blood Luteinizing Hormone - metabolism Luteolysis - drug effects Mammalian female genital system messenger rna oestrogene oestrogens oestrous cycle ovariectomia ovariectomie Ovariectomy oveja ovulacion Ovulation pituitary gland Pituitary Gland, Anterior - drug effects Pituitary Gland, Anterior - metabolism prostaglandinas prostaglandine prostaglandins recepteur d' hormone receptores de hormonas Receptors, LHRH - genetics RNA, Messenger - metabolism Sheep Vertebrates: reproduction |
| title | Estradiol increases amounts of messenger ribonucleic acid for gonadotropin-releasing hormone receptors in sheep |
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