Gene–based sequence–tagged–sites (STSs) as the basis for a human gene map
Using our data set of 3,143 single pass sequences from human brain cDNA libraries, we have developed a strategy in which gene–based sequence–tagged–sites (STSs), derived from 3′untranslated regions of human cDNAs, are rapidly assigned to megabase–insert yeast artificial chromosomes and somatic cell...
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| Veröffentlicht in: | Nature genetics 1995-08, Vol.10 (4), p.415-423 |
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| container_title | Nature genetics |
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| creator | Berry, Rebecca Stevens, T.J. Walter, Nicole A.R. Wilcox, Andrea S. Rubano, Todd Hopkins, Janet A. Weber, James Goold, Richard Soares, Marcelo Bento Sikela, James M. |
| description | Using our data set of 3,143 single pass sequences from human brain cDNA libraries, we have developed a strategy in which gene–based sequence–tagged–sites (STSs), derived from 3′untranslated regions of human cDNAs, are rapidly assigned to megabase–insert yeast artificial chromosomes and somatic cell hybrids to generate regional gene mapping data. Employing this approach, we have mapped 318 cDNAs, representing 308 human genes. Ninety–two of these mapped to regions implicated in human genetic diseases, identifying them as candidate genes. Extension of this strategy has the potential to result in virtually every human gene having, at its 3′ end, its own associated STS, with each STS in turn specifying both a corresponding genomic clone and a specific regional location in the genome. |
| doi_str_mv | 10.1038/ng0895-415 |
| format | Article |
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Employing this approach, we have mapped 318 cDNAs, representing 308 human genes. Ninety–two of these mapped to regions implicated in human genetic diseases, identifying them as candidate genes. 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| ispartof | Nature genetics, 1995-08, Vol.10 (4), p.415-423 |
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| source | MEDLINE; Springer Nature - Complete Springer Journals; Nature Journals Online |
| subjects | Agriculture Animal Genetics and Genomics Animals Biological and medical sciences Biomedical and Life Sciences Biomedicine Brain Cancer Research Chromosome Mapping - methods Chromosomes, Artificial, Yeast Classical genetics, quantitative genetics, hybrids Fundamental and applied biological sciences. Psychology Gene Function Genetics of eukaryotes. Biological and molecular evolution Genome, Human Human Human Genetics Humans Hybrid Cells Polymerase Chain Reaction Rodentia Sequence Tagged Sites |
| title | Gene–based sequence–tagged–sites (STSs) as the basis for a human gene map |
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