Isolation and characterization of the lysozyme-encoding gene from the silkworm Bombyx mori

We have isolated and characterized a Bombyx mori (Bm) cDNA encoding a lysozyme (Lyz). A 90-bp DNA fragment was amplified by PCR using degenerate oligodeoxyribonucleotide primers derived from the known amino acid (aa) sequence of the Bm Lyz. These PCR fragments were used to screen a rat body cDNA lib...

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Veröffentlicht in:	Gene 1995-08, Vol.161 (2), p.199-203
Hauptverfasser:	Lee, Won-Jae, Brey, Paul T.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence amino acid sequences Animals Bacillus licheniformis Base Sequence Bombyx - enzymology Bombyx - genetics Bombyx mori cDNA cloning Chickens complementary DNA cuticle defense defense mechanisms DNA, Complementary - genetics Enzyme Precursors - genetics epidermis evolution experimental infections fat body genbank/l37416 gene expression Genes, Insect hemocytes Insect immunity lys gene lysozyme messenger RNA Molecular Sequence Data Muramidase - genetics nucleotide sequences Polymerase Chain Reaction RNA, Messenger - genetics RNA, Messenger - metabolism sequence analysis Sequence Homology, Amino Acid structural genes up-regulation
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