Isolation and characterization of the lysozyme-encoding gene from the silkworm Bombyx mori
We have isolated and characterized a Bombyx mori (Bm) cDNA encoding a lysozyme (Lyz). A 90-bp DNA fragment was amplified by PCR using degenerate oligodeoxyribonucleotide primers derived from the known amino acid (aa) sequence of the Bm Lyz. These PCR fragments were used to screen a rat body cDNA lib...
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| Veröffentlicht in: | Gene 1995-08, Vol.161 (2), p.199-203 |
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| creator | Lee, Won-Jae Brey, Paul T. |
| description | We have isolated and characterized a
Bombyx mori (Bm) cDNA encoding a lysozyme (Lyz). A 90-bp DNA fragment was amplified by PCR using degenerate oligodeoxyribonucleotide primers derived from the known amino acid (aa) sequence of the
Bm Lyz. These PCR fragments were used to screen a rat body cDNA library. A clone containing the complete
lys cDNA (1294 bp) was isolated and completely sequenced. The deduced 137-aa sequence showed high homology with other chicken-type Lyz.
Bm lys gene expression was constitutive in fat body, cuticular epidermal tissue and at a very low level in hemocytes. This gene expression was up-regulated in fat body, hemocytes and cuticular epidermal tissue following the injection of Gram
+ bacteria. |
| doi_str_mv | 10.1016/0378-1119(95)00199-G |
| format | Article |
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Bombyx mori (Bm) cDNA encoding a lysozyme (Lyz). A 90-bp DNA fragment was amplified by PCR using degenerate oligodeoxyribonucleotide primers derived from the known amino acid (aa) sequence of the
Bm Lyz. These PCR fragments were used to screen a rat body cDNA library. A clone containing the complete
lys cDNA (1294 bp) was isolated and completely sequenced. The deduced 137-aa sequence showed high homology with other chicken-type Lyz.
Bm lys gene expression was constitutive in fat body, cuticular epidermal tissue and at a very low level in hemocytes. This gene expression was up-regulated in fat body, hemocytes and cuticular epidermal tissue following the injection of Gram
+ bacteria.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/0378-1119(95)00199-G</identifier><identifier>PMID: 7665079</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Amino Acid Sequence ; amino acid sequences ; Animals ; Bacillus licheniformis ; Base Sequence ; Bombyx - enzymology ; Bombyx - genetics ; Bombyx mori ; cDNA cloning ; Chickens ; complementary DNA ; cuticle ; defense ; defense mechanisms ; DNA, Complementary - genetics ; Enzyme Precursors - genetics ; epidermis ; evolution ; experimental infections ; fat body ; genbank/l37416 ; gene expression ; Genes, Insect ; hemocytes ; Insect immunity ; lys gene ; lysozyme ; messenger RNA ; Molecular Sequence Data ; Muramidase - genetics ; nucleotide sequences ; Polymerase Chain Reaction ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; sequence analysis ; Sequence Homology, Amino Acid ; structural genes ; up-regulation</subject><ispartof>Gene, 1995-08, Vol.161 (2), p.199-203</ispartof><rights>1995</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c529t-ad4fb779b2aa08fa5116a1879f0d04c4ec2bcaf9f5f18b844cf8f7d153ecc6da3</citedby><cites>FETCH-LOGICAL-c529t-ad4fb779b2aa08fa5116a1879f0d04c4ec2bcaf9f5f18b844cf8f7d153ecc6da3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0378-1119(95)00199-G$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7665079$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lee, Won-Jae</creatorcontrib><creatorcontrib>Brey, Paul T.</creatorcontrib><title>Isolation and characterization of the lysozyme-encoding gene from the silkworm Bombyx mori</title><title>Gene</title><addtitle>Gene</addtitle><description>We have isolated and characterized a
Bombyx mori (Bm) cDNA encoding a lysozyme (Lyz). A 90-bp DNA fragment was amplified by PCR using degenerate oligodeoxyribonucleotide primers derived from the known amino acid (aa) sequence of the
Bm Lyz. These PCR fragments were used to screen a rat body cDNA library. A clone containing the complete
lys cDNA (1294 bp) was isolated and completely sequenced. The deduced 137-aa sequence showed high homology with other chicken-type Lyz.
Bm lys gene expression was constitutive in fat body, cuticular epidermal tissue and at a very low level in hemocytes. This gene expression was up-regulated in fat body, hemocytes and cuticular epidermal tissue following the injection of Gram
+ bacteria.</description><subject>Amino Acid Sequence</subject><subject>amino acid sequences</subject><subject>Animals</subject><subject>Bacillus licheniformis</subject><subject>Base Sequence</subject><subject>Bombyx - enzymology</subject><subject>Bombyx - genetics</subject><subject>Bombyx mori</subject><subject>cDNA cloning</subject><subject>Chickens</subject><subject>complementary DNA</subject><subject>cuticle</subject><subject>defense</subject><subject>defense mechanisms</subject><subject>DNA, Complementary - genetics</subject><subject>Enzyme Precursors - genetics</subject><subject>epidermis</subject><subject>evolution</subject><subject>experimental infections</subject><subject>fat body</subject><subject>genbank/l37416</subject><subject>gene expression</subject><subject>Genes, Insect</subject><subject>hemocytes</subject><subject>Insect immunity</subject><subject>lys gene</subject><subject>lysozyme</subject><subject>messenger RNA</subject><subject>Molecular Sequence Data</subject><subject>Muramidase - genetics</subject><subject>nucleotide sequences</subject><subject>Polymerase Chain Reaction</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>sequence analysis</subject><subject>Sequence Homology, Amino Acid</subject><subject>structural genes</subject><subject>up-regulation</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMFu1DAQhq0K1C6FN2hFTggOKTNJHMcXJKhgqVSpB-iFi-XY463bJC52lnb79GQ3qx7Bl5H8fzP2fIydIJwhYP0RStHkiCjfS_4BAKXMlwdsgY2QOUDZvGCLZ-SIvUrpFqbDeXHIDkVdcxBywX5dpNDp0Ych04PNzI2O2owU_dN8GVw23lDWbVJ42vSU02CC9cMqW9FAmYuh3-XJd3cPIfbZl9C3m8esD9G_Zi-d7hK92ddjdv3t68_z7_nl1fLi_PNlbnghx1zbyrVCyLbQGhqnOWKtt0s4sFCZikzRGu2k4w6btqkq4xonLPKSjKmtLo_Zu3nufQy_15RG1ftkqOv0QGGdlBCVrGUl_wuiAF4UdTmB1QyaGFKK5NR99L2OG4Wgtu7VVqzailWSq517tZzaTvfz121P9rlpL3vK386500HpVfRJXf8oAEtADg3uHv40EzT5-uMpqmT8pJysj2RGZYP_9xf-AhVjnl8</recordid><startdate>19950819</startdate><enddate>19950819</enddate><creator>Lee, Won-Jae</creator><creator>Brey, Paul T.</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19950819</creationdate><title>Isolation and characterization of the lysozyme-encoding gene from the silkworm Bombyx mori</title><author>Lee, Won-Jae ; Brey, Paul T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c529t-ad4fb779b2aa08fa5116a1879f0d04c4ec2bcaf9f5f18b844cf8f7d153ecc6da3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Amino Acid Sequence</topic><topic>amino acid sequences</topic><topic>Animals</topic><topic>Bacillus licheniformis</topic><topic>Base Sequence</topic><topic>Bombyx - enzymology</topic><topic>Bombyx - genetics</topic><topic>Bombyx mori</topic><topic>cDNA cloning</topic><topic>Chickens</topic><topic>complementary DNA</topic><topic>cuticle</topic><topic>defense</topic><topic>defense mechanisms</topic><topic>DNA, Complementary - genetics</topic><topic>Enzyme Precursors - genetics</topic><topic>epidermis</topic><topic>evolution</topic><topic>experimental infections</topic><topic>fat body</topic><topic>genbank/l37416</topic><topic>gene expression</topic><topic>Genes, Insect</topic><topic>hemocytes</topic><topic>Insect immunity</topic><topic>lys gene</topic><topic>lysozyme</topic><topic>messenger RNA</topic><topic>Molecular Sequence Data</topic><topic>Muramidase - genetics</topic><topic>nucleotide sequences</topic><topic>Polymerase Chain Reaction</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>sequence analysis</topic><topic>Sequence Homology, Amino Acid</topic><topic>structural genes</topic><topic>up-regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, Won-Jae</creatorcontrib><creatorcontrib>Brey, Paul T.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, Won-Jae</au><au>Brey, Paul T.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and characterization of the lysozyme-encoding gene from the silkworm Bombyx mori</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>1995-08-19</date><risdate>1995</risdate><volume>161</volume><issue>2</issue><spage>199</spage><epage>203</epage><pages>199-203</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><abstract>We have isolated and characterized a
Bombyx mori (Bm) cDNA encoding a lysozyme (Lyz). A 90-bp DNA fragment was amplified by PCR using degenerate oligodeoxyribonucleotide primers derived from the known amino acid (aa) sequence of the
Bm Lyz. These PCR fragments were used to screen a rat body cDNA library. A clone containing the complete
lys cDNA (1294 bp) was isolated and completely sequenced. The deduced 137-aa sequence showed high homology with other chicken-type Lyz.
Bm lys gene expression was constitutive in fat body, cuticular epidermal tissue and at a very low level in hemocytes. This gene expression was up-regulated in fat body, hemocytes and cuticular epidermal tissue following the injection of Gram
+ bacteria.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>7665079</pmid><doi>10.1016/0378-1119(95)00199-G</doi><tpages>5</tpages></addata></record> |
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| ispartof | Gene, 1995-08, Vol.161 (2), p.199-203 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Amino Acid Sequence amino acid sequences Animals Bacillus licheniformis Base Sequence Bombyx - enzymology Bombyx - genetics Bombyx mori cDNA cloning Chickens complementary DNA cuticle defense defense mechanisms DNA, Complementary - genetics Enzyme Precursors - genetics epidermis evolution experimental infections fat body genbank/l37416 gene expression Genes, Insect hemocytes Insect immunity lys gene lysozyme messenger RNA Molecular Sequence Data Muramidase - genetics nucleotide sequences Polymerase Chain Reaction RNA, Messenger - genetics RNA, Messenger - metabolism sequence analysis Sequence Homology, Amino Acid structural genes up-regulation |
| title | Isolation and characterization of the lysozyme-encoding gene from the silkworm Bombyx mori |
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