Increased exon-trapping efficiency through modifications to the pSPL3 splicing vector

Exon trapping allows for the rapid identification and cloning of coding regions from cloned eukaryotic DNA. In preliminary experiments, we observed two phenomena which limited the exon-trapping efficiency of pSPL3-based systems. The first factor that affected performance was revealed when we found t...

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Veröffentlicht in:	Gene 1995-08, Vol.161 (2), p.183-187
Hauptverfasser:	Burn, Timothy C., Connors, Timothy D., Klinger, Katherine W., Landes, Gregory M.
Format:	Artikel
Sprache:	eng
Schlagworte:	AIDS/HIV Base Sequence Chromosomes, Human, Pair 16 cloning Cloning, Molecular Cosmids DNA - genetics DNA Primers - genetics DNA, Recombinant exon amplification Exons Gene identification Genes, tat Genetic Vectors HIV - genetics human Humans Molecular Sequence Data
Online-Zugang:	Volltext
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