Detection of cutaneous Leishmania infection in paraffin-embedded skin biopsies using the polymerase chain reaction
In this study the polymerase chain reaction (PCR) with previously developed oligonucleotide primers was used to detect Leishmania aethiopica in paraffin-embedded skin biopsy specimens. The Leishmania-specific 120 base pair fragment of the kinetoplast deoxyribonucleic acid (kDNA) minicircles has been...
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| Veröffentlicht in: | Transactions of the Royal Society of Tropical Medicine and Hygiene 1995-05, Vol.89 (3), p.273-275 |
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| creator | Laskay, Tamás Mikó, Tivadar L. Negesse, Yohannes Solbach, Werner Röllinghoff, Martin Frommel, Dominique |
| description | In this study the polymerase chain reaction (PCR) with previously developed oligonucleotide primers was used to detect
Leishmania aethiopica in paraffin-embedded skin biopsy specimens. The
Leishmania-specific 120 base pair fragment of the kinetoplast deoxyribonucleic acid (kDNA) minicircles has been amplified from all parasitologically or histologically confirmed cases of cutaneous leishmaniasis (CL), as demonstrated by gel electrophoresis and hybridization with
L. aethiopica kDNA. Control specimens from patients with skin diseases other than CL were all negative. Using PCR,
Leishmania were demonstrated in the skin lesions of 7 cases in a group of 40 patients in whom the parasites could not be demonstrated by histopathology or culture
in vitro although lesions were clinically suggestive of CL. These data indicate that PCR, carried out on DNA extracted from formalin-fixed and paraffin-embedded tissue specimens, is a valuable method for the diagnosis of CL, especially in chronic cases where the parasite load in the lesion is low. |
| doi_str_mv | 10.1016/0035-9203(95)90537-5 |
| format | Article |
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Leishmania aethiopica in paraffin-embedded skin biopsy specimens. The
Leishmania-specific 120 base pair fragment of the kinetoplast deoxyribonucleic acid (kDNA) minicircles has been amplified from all parasitologically or histologically confirmed cases of cutaneous leishmaniasis (CL), as demonstrated by gel electrophoresis and hybridization with
L. aethiopica kDNA. Control specimens from patients with skin diseases other than CL were all negative. Using PCR,
Leishmania were demonstrated in the skin lesions of 7 cases in a group of 40 patients in whom the parasites could not be demonstrated by histopathology or culture
in vitro although lesions were clinically suggestive of CL. These data indicate that PCR, carried out on DNA extracted from formalin-fixed and paraffin-embedded tissue specimens, is a valuable method for the diagnosis of CL, especially in chronic cases where the parasite load in the lesion is low.</description><identifier>ISSN: 0035-9203</identifier><identifier>EISSN: 1878-3503</identifier><identifier>DOI: 10.1016/0035-9203(95)90537-5</identifier><identifier>PMID: 7660431</identifier><identifier>CODEN: TRSTAZ</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Animals ; Base Sequence ; Biological and medical sciences ; cutaneous leishmaniasis ; detection in paraffin sections ; Human protozoal diseases ; Humans ; Infectious diseases ; Leishmania - isolation & purification ; Leishmania aethiopica ; leishmaniasis ; Leishmaniasis, Cutaneous - diagnosis ; Leishmaniasis, Cutaneous - pathology ; Leshmaniasis ; Medical sciences ; Molecular Sequence Data ; Parasitic diseases ; Parasitology - methods ; Polymerase Chain Reaction ; Protozoal diseases ; Skin - parasitology ; Skin - pathology ; Tropical medicine</subject><ispartof>Transactions of the Royal Society of Tropical Medicine and Hygiene, 1995-05, Vol.89 (3), p.273-275</ispartof><rights>1995</rights><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-5840aaf54501c935c5f82f1457379e3fd5e0f9e43befa6807f89552d6480dd543</citedby><cites>FETCH-LOGICAL-c356t-5840aaf54501c935c5f82f1457379e3fd5e0f9e43befa6807f89552d6480dd543</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>309,310,314,780,784,789,790,23930,23931,25140,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3589576$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7660431$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Laskay, Tamás</creatorcontrib><creatorcontrib>Mikó, Tivadar L.</creatorcontrib><creatorcontrib>Negesse, Yohannes</creatorcontrib><creatorcontrib>Solbach, Werner</creatorcontrib><creatorcontrib>Röllinghoff, Martin</creatorcontrib><creatorcontrib>Frommel, Dominique</creatorcontrib><title>Detection of cutaneous Leishmania infection in paraffin-embedded skin biopsies using the polymerase chain reaction</title><title>Transactions of the Royal Society of Tropical Medicine and Hygiene</title><addtitle>Trans R Soc Trop Med Hyg</addtitle><description>In this study the polymerase chain reaction (PCR) with previously developed oligonucleotide primers was used to detect
Leishmania aethiopica in paraffin-embedded skin biopsy specimens. The
Leishmania-specific 120 base pair fragment of the kinetoplast deoxyribonucleic acid (kDNA) minicircles has been amplified from all parasitologically or histologically confirmed cases of cutaneous leishmaniasis (CL), as demonstrated by gel electrophoresis and hybridization with
L. aethiopica kDNA. Control specimens from patients with skin diseases other than CL were all negative. Using PCR,
Leishmania were demonstrated in the skin lesions of 7 cases in a group of 40 patients in whom the parasites could not be demonstrated by histopathology or culture
in vitro although lesions were clinically suggestive of CL. These data indicate that PCR, carried out on DNA extracted from formalin-fixed and paraffin-embedded tissue specimens, is a valuable method for the diagnosis of CL, especially in chronic cases where the parasite load in the lesion is low.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>cutaneous leishmaniasis</subject><subject>detection in paraffin sections</subject><subject>Human protozoal diseases</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Leishmania - isolation & purification</subject><subject>Leishmania aethiopica</subject><subject>leishmaniasis</subject><subject>Leishmaniasis, Cutaneous - diagnosis</subject><subject>Leishmaniasis, Cutaneous - pathology</subject><subject>Leshmaniasis</subject><subject>Medical sciences</subject><subject>Molecular Sequence Data</subject><subject>Parasitic diseases</subject><subject>Parasitology - methods</subject><subject>Polymerase Chain Reaction</subject><subject>Protozoal diseases</subject><subject>Skin - parasitology</subject><subject>Skin - pathology</subject><subject>Tropical medicine</subject><issn>0035-9203</issn><issn>1878-3503</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU2LFDEQhoMo67j6DxRyENFDa9JJdXcugqwfIwx4WWXxEjJJxYnbXybd4v5703YzR08FeZ96U_UWIU85e80Zr94wJqBQJRMvFbxSDERdwD2y403dFAKYuE92Z-QheZTST8ZK4KAuyEVdVUwKviPxPU5opzD0dPDUzpPpcZgTPWBIp870wdDQ-40IPR1NNN6HvsDuiM6ho-k2Px_DMKaAic4p9D_odEI6Du1dh9EkpPZkMhPR_LN5TB540yZ8stVL8vXjh-urfXH48unz1btDYQVUUwGNZMZ4kMC4VQIs-Kb0XEItaoXCO0DmFUpxRG-qhtW-UQClq2TDnAMpLsmL1XeMw68Z06S7kCy27bqirmupgJdVBuUK2jikFNHrMYbOxDvNmV6i1kuOeslRq1yXqDXktmeb_3zs0J2btmyz_nzTTbKm9dH0NqQzJiDPWy-_FysW0oR_zrKJt7rKq4Le33zXNxIa2H-71k3m36485ux-B4w62YC9RRdivpN2Q_j_3H8Bp4mqig</recordid><startdate>19950501</startdate><enddate>19950501</enddate><creator>Laskay, Tamás</creator><creator>Mikó, Tivadar L.</creator><creator>Negesse, Yohannes</creator><creator>Solbach, Werner</creator><creator>Röllinghoff, Martin</creator><creator>Frommel, Dominique</creator><general>Elsevier Ltd</general><general>Royal Society of Tropical Medicine and Hygiene</general><general>Elsevier</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19950501</creationdate><title>Detection of cutaneous Leishmania infection in paraffin-embedded skin biopsies using the polymerase chain reaction</title><author>Laskay, Tamás ; Mikó, Tivadar L. ; Negesse, Yohannes ; Solbach, Werner ; Röllinghoff, Martin ; Frommel, Dominique</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-5840aaf54501c935c5f82f1457379e3fd5e0f9e43befa6807f89552d6480dd543</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>cutaneous leishmaniasis</topic><topic>detection in paraffin sections</topic><topic>Human protozoal diseases</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>Leishmania - isolation & purification</topic><topic>Leishmania aethiopica</topic><topic>leishmaniasis</topic><topic>Leishmaniasis, Cutaneous - diagnosis</topic><topic>Leishmaniasis, Cutaneous - pathology</topic><topic>Leshmaniasis</topic><topic>Medical sciences</topic><topic>Molecular Sequence Data</topic><topic>Parasitic diseases</topic><topic>Parasitology - methods</topic><topic>Polymerase Chain Reaction</topic><topic>Protozoal diseases</topic><topic>Skin - parasitology</topic><topic>Skin - pathology</topic><topic>Tropical medicine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Laskay, Tamás</creatorcontrib><creatorcontrib>Mikó, Tivadar L.</creatorcontrib><creatorcontrib>Negesse, Yohannes</creatorcontrib><creatorcontrib>Solbach, Werner</creatorcontrib><creatorcontrib>Röllinghoff, Martin</creatorcontrib><creatorcontrib>Frommel, Dominique</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Transactions of the Royal Society of Tropical Medicine and Hygiene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Laskay, Tamás</au><au>Mikó, Tivadar L.</au><au>Negesse, Yohannes</au><au>Solbach, Werner</au><au>Röllinghoff, Martin</au><au>Frommel, Dominique</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of cutaneous Leishmania infection in paraffin-embedded skin biopsies using the polymerase chain reaction</atitle><jtitle>Transactions of the Royal Society of Tropical Medicine and Hygiene</jtitle><addtitle>Trans R Soc Trop Med Hyg</addtitle><date>1995-05-01</date><risdate>1995</risdate><volume>89</volume><issue>3</issue><spage>273</spage><epage>275</epage><pages>273-275</pages><issn>0035-9203</issn><eissn>1878-3503</eissn><coden>TRSTAZ</coden><abstract>In this study the polymerase chain reaction (PCR) with previously developed oligonucleotide primers was used to detect
Leishmania aethiopica in paraffin-embedded skin biopsy specimens. The
Leishmania-specific 120 base pair fragment of the kinetoplast deoxyribonucleic acid (kDNA) minicircles has been amplified from all parasitologically or histologically confirmed cases of cutaneous leishmaniasis (CL), as demonstrated by gel electrophoresis and hybridization with
L. aethiopica kDNA. Control specimens from patients with skin diseases other than CL were all negative. Using PCR,
Leishmania were demonstrated in the skin lesions of 7 cases in a group of 40 patients in whom the parasites could not be demonstrated by histopathology or culture
in vitro although lesions were clinically suggestive of CL. These data indicate that PCR, carried out on DNA extracted from formalin-fixed and paraffin-embedded tissue specimens, is a valuable method for the diagnosis of CL, especially in chronic cases where the parasite load in the lesion is low.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>7660431</pmid><doi>10.1016/0035-9203(95)90537-5</doi><tpages>3</tpages></addata></record> |
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| identifier | ISSN: 0035-9203 |
| ispartof | Transactions of the Royal Society of Tropical Medicine and Hygiene, 1995-05, Vol.89 (3), p.273-275 |
| issn | 0035-9203 1878-3503 |
| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Base Sequence Biological and medical sciences cutaneous leishmaniasis detection in paraffin sections Human protozoal diseases Humans Infectious diseases Leishmania - isolation & purification Leishmania aethiopica leishmaniasis Leishmaniasis, Cutaneous - diagnosis Leishmaniasis, Cutaneous - pathology Leshmaniasis Medical sciences Molecular Sequence Data Parasitic diseases Parasitology - methods Polymerase Chain Reaction Protozoal diseases Skin - parasitology Skin - pathology Tropical medicine |
| title | Detection of cutaneous Leishmania infection in paraffin-embedded skin biopsies using the polymerase chain reaction |
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