Suppression of human melanoma cell growth and metastasis by the melanoma‐associated antigen CD63 (ME491)
CD63 has been identified in human melanoma cells by a number of different monoclonal antibodies (MAbs). Studies with MAbs have shown that expression is most marked in naevi and early forms of cutaneous melanoma and reduced in vertical growth phase and metastatic lesions. To investigate further the r...
Gespeichert in:
| Veröffentlicht in: | International journal of cancer 1995-09, Vol.62 (5), p.631-635 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 635 |
|---|---|
| container_issue | 5 |
| container_start_page | 631 |
| container_title | International journal of cancer |
| container_volume | 62 |
| creator | Radford, Kristen J. Mallesch, Julia Mersey, Peter |
| description | CD63 has been identified in human melanoma cells by a number of different monoclonal antibodies (MAbs). Studies with MAbs have shown that expression is most marked in naevi and early forms of cutaneous melanoma and reduced in vertical growth phase and metastatic lesions. To investigate further the role of CD63 in progression of melanoma, genomic CD63 was transfected into a CD63‐negative human melanoma cell line using an episomal vector. The stable transfected melanoma cells had similar growth rates to control transfected melanoma cells in vitro but much lower growth rates when injected intradermally into athymic nude mice. The CD63‐transfected cells also had a reduced number of metastases in the peritoneal cavity and subcutaneous sites when injected intravenously. MAb against CD63 did not influence the growth of CO63‐transfected melanoma cells in vitro. Our results confirm previous studies using H‐ros‐transformed NIH3T3 fibroblasts and suggest that CD63 may have a role as a tumor suppressor gene in human melanoma that acts to limit invasion and progression of melanoma. © 1995 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/ijc.2910620523 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_77494611</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>77494611</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4353-d264c582cce20d5fa2c577e9ab417d446d8521e2aa7f8bffbc959c5a93347d03</originalsourceid><addsrcrecordid>eNqF0MtKAzEUBuAgSq2XrTshCxFdTM09zVLqHcWF3Q9nMhmbMpc6mUG68xF8Rp_ESEt1JwSy-L-cJD9CR5SMKCHsws_tiBlKFCOS8S00pMTohDAqt9EwApJoytUu2gthTgilkogBGmilotZDNH_pF4vWheCbGjcFnvUV1LhyJdRNBdi6ssSvbfPezTDUeQw6CHH5gLMl7mZuQ78-PiGExnroXB5t519djSdXiuOzp2th6PkB2imgDO5wve-j6c31dHKXPD7f3k8uHxMruORJzpSwcsysdYzksgBmpdbOQCaozoVQ-Vgy6hiALsZZUWTWSGMlGM6FzgnfR6ersYu2eetd6NLKh59_QO2aPqRaCyMUpRGOVtC2TQitK9JF6ytolykl6U-3aew2_e02HjheT-6zyuUbvi4z5ifrHIKFsmihtj5sGFdC8bGMzKzYuy_d8p9L0_uHyZ8nfANEqpKx</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>77494611</pqid></control><display><type>article</type><title>Suppression of human melanoma cell growth and metastasis by the melanoma‐associated antigen CD63 (ME491)</title><source>MEDLINE</source><source>Access via Wiley Online Library</source><creator>Radford, Kristen J. ; Mallesch, Julia ; Mersey, Peter</creator><creatorcontrib>Radford, Kristen J. ; Mallesch, Julia ; Mersey, Peter</creatorcontrib><description>CD63 has been identified in human melanoma cells by a number of different monoclonal antibodies (MAbs). Studies with MAbs have shown that expression is most marked in naevi and early forms of cutaneous melanoma and reduced in vertical growth phase and metastatic lesions. To investigate further the role of CD63 in progression of melanoma, genomic CD63 was transfected into a CD63‐negative human melanoma cell line using an episomal vector. The stable transfected melanoma cells had similar growth rates to control transfected melanoma cells in vitro but much lower growth rates when injected intradermally into athymic nude mice. The CD63‐transfected cells also had a reduced number of metastases in the peritoneal cavity and subcutaneous sites when injected intravenously. MAb against CD63 did not influence the growth of CO63‐transfected melanoma cells in vitro. Our results confirm previous studies using H‐ros‐transformed NIH3T3 fibroblasts and suggest that CD63 may have a role as a tumor suppressor gene in human melanoma that acts to limit invasion and progression of melanoma. © 1995 Wiley‐Liss, Inc.</description><identifier>ISSN: 0020-7136</identifier><identifier>EISSN: 1097-0215</identifier><identifier>DOI: 10.1002/ijc.2910620523</identifier><identifier>PMID: 7665237</identifier><identifier>CODEN: IJCNAW</identifier><language>eng</language><publisher>New York: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Animal tumors. Experimental tumors ; Animals ; Antigens, CD - physiology ; Base Sequence ; Biological and medical sciences ; Cell Division ; DNA Primers - chemistry ; Experimental skin tumors ; Genes, Tumor Suppressor ; Medical sciences ; Melanoma - pathology ; Mice ; Mice, Nude ; Molecular Sequence Data ; Neoplasm Metastasis ; Neoplasm Transplantation ; Platelet Membrane Glycoproteins - physiology ; Tetraspanin 30 ; Transfection ; Transplantation, Heterologous ; Tumors</subject><ispartof>International journal of cancer, 1995-09, Vol.62 (5), p.631-635</ispartof><rights>Copyright © 1995 Wiley‐Liss, Inc., A Wiley Company</rights><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4353-d264c582cce20d5fa2c577e9ab417d446d8521e2aa7f8bffbc959c5a93347d03</citedby><cites>FETCH-LOGICAL-c4353-d264c582cce20d5fa2c577e9ab417d446d8521e2aa7f8bffbc959c5a93347d03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fijc.2910620523$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fijc.2910620523$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3646385$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7665237$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Radford, Kristen J.</creatorcontrib><creatorcontrib>Mallesch, Julia</creatorcontrib><creatorcontrib>Mersey, Peter</creatorcontrib><title>Suppression of human melanoma cell growth and metastasis by the melanoma‐associated antigen CD63 (ME491)</title><title>International journal of cancer</title><addtitle>Int J Cancer</addtitle><description>CD63 has been identified in human melanoma cells by a number of different monoclonal antibodies (MAbs). Studies with MAbs have shown that expression is most marked in naevi and early forms of cutaneous melanoma and reduced in vertical growth phase and metastatic lesions. To investigate further the role of CD63 in progression of melanoma, genomic CD63 was transfected into a CD63‐negative human melanoma cell line using an episomal vector. The stable transfected melanoma cells had similar growth rates to control transfected melanoma cells in vitro but much lower growth rates when injected intradermally into athymic nude mice. The CD63‐transfected cells also had a reduced number of metastases in the peritoneal cavity and subcutaneous sites when injected intravenously. MAb against CD63 did not influence the growth of CO63‐transfected melanoma cells in vitro. Our results confirm previous studies using H‐ros‐transformed NIH3T3 fibroblasts and suggest that CD63 may have a role as a tumor suppressor gene in human melanoma that acts to limit invasion and progression of melanoma. © 1995 Wiley‐Liss, Inc.</description><subject>Animal tumors. Experimental tumors</subject><subject>Animals</subject><subject>Antigens, CD - physiology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cell Division</subject><subject>DNA Primers - chemistry</subject><subject>Experimental skin tumors</subject><subject>Genes, Tumor Suppressor</subject><subject>Medical sciences</subject><subject>Melanoma - pathology</subject><subject>Mice</subject><subject>Mice, Nude</subject><subject>Molecular Sequence Data</subject><subject>Neoplasm Metastasis</subject><subject>Neoplasm Transplantation</subject><subject>Platelet Membrane Glycoproteins - physiology</subject><subject>Tetraspanin 30</subject><subject>Transfection</subject><subject>Transplantation, Heterologous</subject><subject>Tumors</subject><issn>0020-7136</issn><issn>1097-0215</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0MtKAzEUBuAgSq2XrTshCxFdTM09zVLqHcWF3Q9nMhmbMpc6mUG68xF8Rp_ESEt1JwSy-L-cJD9CR5SMKCHsws_tiBlKFCOS8S00pMTohDAqt9EwApJoytUu2gthTgilkogBGmilotZDNH_pF4vWheCbGjcFnvUV1LhyJdRNBdi6ssSvbfPezTDUeQw6CHH5gLMl7mZuQ78-PiGExnroXB5t519djSdXiuOzp2th6PkB2imgDO5wve-j6c31dHKXPD7f3k8uHxMruORJzpSwcsysdYzksgBmpdbOQCaozoVQ-Vgy6hiALsZZUWTWSGMlGM6FzgnfR6ersYu2eetd6NLKh59_QO2aPqRaCyMUpRGOVtC2TQitK9JF6ytolykl6U-3aew2_e02HjheT-6zyuUbvi4z5ifrHIKFsmihtj5sGFdC8bGMzKzYuy_d8p9L0_uHyZ8nfANEqpKx</recordid><startdate>19950904</startdate><enddate>19950904</enddate><creator>Radford, Kristen J.</creator><creator>Mallesch, Julia</creator><creator>Mersey, Peter</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19950904</creationdate><title>Suppression of human melanoma cell growth and metastasis by the melanoma‐associated antigen CD63 (ME491)</title><author>Radford, Kristen J. ; Mallesch, Julia ; Mersey, Peter</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4353-d264c582cce20d5fa2c577e9ab417d446d8521e2aa7f8bffbc959c5a93347d03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Animal tumors. Experimental tumors</topic><topic>Animals</topic><topic>Antigens, CD - physiology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cell Division</topic><topic>DNA Primers - chemistry</topic><topic>Experimental skin tumors</topic><topic>Genes, Tumor Suppressor</topic><topic>Medical sciences</topic><topic>Melanoma - pathology</topic><topic>Mice</topic><topic>Mice, Nude</topic><topic>Molecular Sequence Data</topic><topic>Neoplasm Metastasis</topic><topic>Neoplasm Transplantation</topic><topic>Platelet Membrane Glycoproteins - physiology</topic><topic>Tetraspanin 30</topic><topic>Transfection</topic><topic>Transplantation, Heterologous</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Radford, Kristen J.</creatorcontrib><creatorcontrib>Mallesch, Julia</creatorcontrib><creatorcontrib>Mersey, Peter</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of cancer</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Radford, Kristen J.</au><au>Mallesch, Julia</au><au>Mersey, Peter</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Suppression of human melanoma cell growth and metastasis by the melanoma‐associated antigen CD63 (ME491)</atitle><jtitle>International journal of cancer</jtitle><addtitle>Int J Cancer</addtitle><date>1995-09-04</date><risdate>1995</risdate><volume>62</volume><issue>5</issue><spage>631</spage><epage>635</epage><pages>631-635</pages><issn>0020-7136</issn><eissn>1097-0215</eissn><coden>IJCNAW</coden><abstract>CD63 has been identified in human melanoma cells by a number of different monoclonal antibodies (MAbs). Studies with MAbs have shown that expression is most marked in naevi and early forms of cutaneous melanoma and reduced in vertical growth phase and metastatic lesions. To investigate further the role of CD63 in progression of melanoma, genomic CD63 was transfected into a CD63‐negative human melanoma cell line using an episomal vector. The stable transfected melanoma cells had similar growth rates to control transfected melanoma cells in vitro but much lower growth rates when injected intradermally into athymic nude mice. The CD63‐transfected cells also had a reduced number of metastases in the peritoneal cavity and subcutaneous sites when injected intravenously. MAb against CD63 did not influence the growth of CO63‐transfected melanoma cells in vitro. Our results confirm previous studies using H‐ros‐transformed NIH3T3 fibroblasts and suggest that CD63 may have a role as a tumor suppressor gene in human melanoma that acts to limit invasion and progression of melanoma. © 1995 Wiley‐Liss, Inc.</abstract><cop>New York</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>7665237</pmid><doi>10.1002/ijc.2910620523</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0020-7136 |
| ispartof | International journal of cancer, 1995-09, Vol.62 (5), p.631-635 |
| issn | 0020-7136 1097-0215 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_77494611 |
| source | MEDLINE; Access via Wiley Online Library |
| subjects | Animal tumors. Experimental tumors Animals Antigens, CD - physiology Base Sequence Biological and medical sciences Cell Division DNA Primers - chemistry Experimental skin tumors Genes, Tumor Suppressor Medical sciences Melanoma - pathology Mice Mice, Nude Molecular Sequence Data Neoplasm Metastasis Neoplasm Transplantation Platelet Membrane Glycoproteins - physiology Tetraspanin 30 Transfection Transplantation, Heterologous Tumors |
| title | Suppression of human melanoma cell growth and metastasis by the melanoma‐associated antigen CD63 (ME491) |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-27T03%3A26%3A39IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Suppression%20of%20human%20melanoma%20cell%20growth%20and%20metastasis%20by%20the%20melanoma%E2%80%90associated%20antigen%20CD63%20(ME491)&rft.jtitle=International%20journal%20of%20cancer&rft.au=Radford,%20Kristen%20J.&rft.date=1995-09-04&rft.volume=62&rft.issue=5&rft.spage=631&rft.epage=635&rft.pages=631-635&rft.issn=0020-7136&rft.eissn=1097-0215&rft.coden=IJCNAW&rft_id=info:doi/10.1002/ijc.2910620523&rft_dat=%3Cproquest_cross%3E77494611%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=77494611&rft_id=info:pmid/7665237&rfr_iscdi=true |