Cytotoxicity of wound dressing materials assessed using cultured skin equivalents

An in vitro system, based on the Bell model of cultured composite skin equivalents, was used to assess the effect of a number of wound dressing materials on DNA synthesis. DNA synthesis was quantified using immunocytohistochemical identification of incorporated bromodeoxyuridine and the percentage o...

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Veröffentlicht in:Burns 1995-06, Vol.21 (4), p.249-254
Hauptverfasser: Sieber, V.K., Otto, W.R., Riches, D.J.
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Otto, W.R.
Riches, D.J.
description An in vitro system, based on the Bell model of cultured composite skin equivalents, was used to assess the effect of a number of wound dressing materials on DNA synthesis. DNA synthesis was quantified using immunocytohistochemical identification of incorporated bromodeoxyuridine and the percentage of labelled cells measured, following 7 days' exposure to the dressing material. Differences in labelling index were observed from replica gels covered by different dressing materials and between dressings of the same type of material, but made by different manufacturers.
doi_str_mv 10.1016/0305-4179(95)00021-3
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source MEDLINE; Elsevier ScienceDirect Journals
subjects Animals
Bandages
Biological and medical sciences
Cells, Cultured
Child, Preschool
Diseases of the skin. Cosmetics
DNA - biosynthesis
Humans
Immunohistochemistry
Medical sciences
Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects)
Rats
Skin - metabolism
Skin - pathology
Skin Transplantation
Skin, Artificial
Wounds and Injuries - metabolism
Wounds and Injuries - pathology
Wounds and Injuries - therapy
title Cytotoxicity of wound dressing materials assessed using cultured skin equivalents
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