Cytotoxicity of wound dressing materials assessed using cultured skin equivalents
An in vitro system, based on the Bell model of cultured composite skin equivalents, was used to assess the effect of a number of wound dressing materials on DNA synthesis. DNA synthesis was quantified using immunocytohistochemical identification of incorporated bromodeoxyuridine and the percentage o...
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Veröffentlicht in: | Burns 1995-06, Vol.21 (4), p.249-254 |
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creator | Sieber, V.K. Otto, W.R. Riches, D.J. |
description | An in vitro system, based on the Bell model of cultured composite skin equivalents, was used to assess the effect of a number of wound dressing materials on DNA synthesis. DNA synthesis was quantified using immunocytohistochemical identification of incorporated bromodeoxyuridine and the percentage of labelled cells measured, following 7 days' exposure to the dressing material. Differences in labelling index were observed from replica gels covered by different dressing materials and between dressings of the same type of material, but made by different manufacturers. |
doi_str_mv | 10.1016/0305-4179(95)00021-3 |
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DNA synthesis was quantified using immunocytohistochemical identification of incorporated bromodeoxyuridine and the percentage of labelled cells measured, following 7 days' exposure to the dressing material. Differences in labelling index were observed from replica gels covered by different dressing materials and between dressings of the same type of material, but made by different manufacturers.</description><identifier>ISSN: 0305-4179</identifier><identifier>EISSN: 1879-1409</identifier><identifier>DOI: 10.1016/0305-4179(95)00021-3</identifier><identifier>PMID: 7662123</identifier><identifier>CODEN: BURND8</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Animals ; Bandages ; Biological and medical sciences ; Cells, Cultured ; Child, Preschool ; Diseases of the skin. Cosmetics ; DNA - biosynthesis ; Humans ; Immunohistochemistry ; Medical sciences ; Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects) ; Rats ; Skin - metabolism ; Skin - pathology ; Skin Transplantation ; Skin, Artificial ; Wounds and Injuries - metabolism ; Wounds and Injuries - pathology ; Wounds and Injuries - therapy</subject><ispartof>Burns, 1995-06, Vol.21 (4), p.249-254</ispartof><rights>1995</rights><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-4cffd5571b4eeac0befafa09e9a0d569e377dddd61cec530077a33e3747111593</citedby><cites>FETCH-LOGICAL-c386t-4cffd5571b4eeac0befafa09e9a0d569e377dddd61cec530077a33e3747111593</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0305-4179(95)00021-3$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3526356$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7662123$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sieber, V.K.</creatorcontrib><creatorcontrib>Otto, W.R.</creatorcontrib><creatorcontrib>Riches, D.J.</creatorcontrib><title>Cytotoxicity of wound dressing materials assessed using cultured skin equivalents</title><title>Burns</title><addtitle>Burns</addtitle><description>An in vitro system, based on the Bell model of cultured composite skin equivalents, was used to assess the effect of a number of wound dressing materials on DNA synthesis. DNA synthesis was quantified using immunocytohistochemical identification of incorporated bromodeoxyuridine and the percentage of labelled cells measured, following 7 days' exposure to the dressing material. Differences in labelling index were observed from replica gels covered by different dressing materials and between dressings of the same type of material, but made by different manufacturers.</description><subject>Animals</subject><subject>Bandages</subject><subject>Biological and medical sciences</subject><subject>Cells, Cultured</subject><subject>Child, Preschool</subject><subject>Diseases of the skin. Cosmetics</subject><subject>DNA - biosynthesis</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Medical sciences</subject><subject>Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects)</subject><subject>Rats</subject><subject>Skin - metabolism</subject><subject>Skin - pathology</subject><subject>Skin Transplantation</subject><subject>Skin, Artificial</subject><subject>Wounds and Injuries - metabolism</subject><subject>Wounds and Injuries - pathology</subject><subject>Wounds and Injuries - therapy</subject><issn>0305-4179</issn><issn>1879-1409</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1rGzEQhkVIcB03_6CFPYSQHLYdWauVdQkE0zYBQwm0ZyFLs0XtetfRaJ3630f-wMfMRcy8zwziYewThy8ceP0VBMiy4krfankHAFNeijM25jOlS16BPmfjE_KBXRL9zRDIGYzYSNX1lE_FmD3Pt6lP_f_gQtoWfVO89kPnCx-RKHR_ipVNGINtqbBEeYa-GPaBG9o0xNzSv9AV-DKEjW2xS_SRXTSZx6vjO2G_v3_7NX8sFz9_PM0fFqUTszqVlWsaL6XiywrROlhiYxsLGrUFL2uNQimfq-YOnRQASlkh8rRSnHOpxYTdHO6uY_8yICWzCuSwbW2H_UBGqUpXIEQGqwPoYk8UsTHrGFY2bg0HszNpdprMTpPR0uxNmt3a5-P9YblCf1o6qsv59TG35GzbRNu5QCdMyGktZJ2x-wOG2cUmYDTkAnYOfYjokvF9eP8fb3rSkNU</recordid><startdate>19950601</startdate><enddate>19950601</enddate><creator>Sieber, V.K.</creator><creator>Otto, W.R.</creator><creator>Riches, D.J.</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19950601</creationdate><title>Cytotoxicity of wound dressing materials assessed using cultured skin equivalents</title><author>Sieber, V.K. ; Otto, W.R. ; Riches, D.J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-4cffd5571b4eeac0befafa09e9a0d569e377dddd61cec530077a33e3747111593</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Animals</topic><topic>Bandages</topic><topic>Biological and medical sciences</topic><topic>Cells, Cultured</topic><topic>Child, Preschool</topic><topic>Diseases of the skin. Cosmetics</topic><topic>DNA - biosynthesis</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Medical sciences</topic><topic>Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects)</topic><topic>Rats</topic><topic>Skin - metabolism</topic><topic>Skin - pathology</topic><topic>Skin Transplantation</topic><topic>Skin, Artificial</topic><topic>Wounds and Injuries - metabolism</topic><topic>Wounds and Injuries - pathology</topic><topic>Wounds and Injuries - therapy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sieber, V.K.</creatorcontrib><creatorcontrib>Otto, W.R.</creatorcontrib><creatorcontrib>Riches, D.J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Burns</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sieber, V.K.</au><au>Otto, W.R.</au><au>Riches, D.J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cytotoxicity of wound dressing materials assessed using cultured skin equivalents</atitle><jtitle>Burns</jtitle><addtitle>Burns</addtitle><date>1995-06-01</date><risdate>1995</risdate><volume>21</volume><issue>4</issue><spage>249</spage><epage>254</epage><pages>249-254</pages><issn>0305-4179</issn><eissn>1879-1409</eissn><coden>BURND8</coden><abstract>An in vitro system, based on the Bell model of cultured composite skin equivalents, was used to assess the effect of a number of wound dressing materials on DNA synthesis. DNA synthesis was quantified using immunocytohistochemical identification of incorporated bromodeoxyuridine and the percentage of labelled cells measured, following 7 days' exposure to the dressing material. Differences in labelling index were observed from replica gels covered by different dressing materials and between dressings of the same type of material, but made by different manufacturers.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>7662123</pmid><doi>10.1016/0305-4179(95)00021-3</doi><tpages>6</tpages></addata></record> |
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subjects | Animals Bandages Biological and medical sciences Cells, Cultured Child, Preschool Diseases of the skin. Cosmetics DNA - biosynthesis Humans Immunohistochemistry Medical sciences Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects) Rats Skin - metabolism Skin - pathology Skin Transplantation Skin, Artificial Wounds and Injuries - metabolism Wounds and Injuries - pathology Wounds and Injuries - therapy |
title | Cytotoxicity of wound dressing materials assessed using cultured skin equivalents |
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