The expression, affinity purification and characterization of recombinant pseudomonas exotoxin 40 (pe40) secreted from escherichia coli

Procedures have been devised for producing high yields of purified recombinant PE40, a protein important for the development of the anti-AIDS therapeutic, sCD4-PE40. PE40 is a truncated form of the bacterial toxin, Pseudomonas exotoxin A; it lacks the cell-binding domain, but retains domains II and...

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Veröffentlicht in:	Journal of biotechnology 1995-08, Vol.42 (1), p.9-22
Hauptverfasser:	Kawooya, John K., Treat, Jeanne C., Kirschner, Richard J., Sears, Martha W., Gorczany, auJonathan F., Grode, Stephen H., Strother, Diane S., Asmus, Paul A., Eckenrode, Frances M.
Format:	Artikel
Sprache:	eng
Schlagworte:	ADP Ribose Transferases Amino Acid Sequence Amino Acids - analysis Bacterial Toxins - biosynthesis Bacterial Toxins - chemistry Bacterial Toxins - genetics Bacterial Toxins - isolation & purification Biological and medical sciences Biotechnology Chromatography, Affinity Cibacron blue F3GA Culture Media Disulfides - chemistry Electrospray ionization mass spectrometry Escherichia coli Escherichia coli - genetics Escherichia coli - growth & development Exotoxins - biosynthesis Exotoxins - chemistry Exotoxins - genetics Exotoxins - isolation & purification Expression optimization Fundamental and applied biological sciences. Psychology Gene Expression Gene Expression Regulation - genetics Genetic engineering Genetic technics Isoelectric Focusing Methods. Procedures. Technologies Modification of gene expression level Molecular Sequence Data Peptide Mapping Peptides - chemistry Promoter Regions, Genetic Pseudomonas Pseudomonas aeruginosa - metabolism Pseudomonas aeruginosa Exotoxin A Pseudomonas exotoxin A Recombinant Proteins - biosynthesis Recombinant Proteins - chemistry Recombinant Proteins - isolation & purification Trypsin - metabolism Virulence Factors
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