Use of genetically engineered lipid-tagged antibody to generate functional europium chelate-loaded liposomes Application in fluoroimmunoassay

Biosynthetically lipid-tagged single-chain antibody (Laukkanen et al., Protein Eng. 6 (1993) 449; Biochemistry 33 (1994) 11664) has been used to functionalize europium (Eu 3+) chelate-loaded liposomes. The resulting Eu immunoliposomes displayed specific hapten-binding activity and little non-specifi...

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Veröffentlicht in:	Journal of immunological methods 1995-09, Vol.185 (1), p.95-102
Hauptverfasser:	Laukkanen, Marja-Leena, Orellana, Adelina, Keinänen, Kari
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibodies - chemistry Antibody engineering Biological and medical sciences Europium - administration & dosage Europium chelate Fluorescent Antibody Technique Fluorescent Dyes Fundamental and applied biological sciences. Psychology Fundamental immunology Genetic Engineering Haptens Immunoglobulin Fragments - chemistry Immunoliposome Lanthanide Lipid-tagged antibody Lipids Liposomes Molecular immunology Pentetic Acid - chemistry Recombinant Proteins Techniques Time-resolved fluorometry
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