Methamphetamine-induced dopaminergic hyperactivity is not accompanied with increase in tyrosine hydroxylase mRNA of the rat midbrain
Tyrosine hydroxylase (TH) mRNA was measured in the midbrain of rats treated repeatedly with methamphetamine (MAP). Male Sprague-Dawley rats were daily injected with MAP (5 mg/kg, i.p., once daily) or saline for 14 days. Progressive augmentation was observed in MAP-induced stereotyped behaviors. Afte...
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| Veröffentlicht in: | Neuroscience letters 1995-05, Vol.191 (1), p.107-110 |
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| creator | Ishimaru, Masahiko Hashimoto, Takanori Obata, Kunihiko Fukamauchi, Fumihiko |
| description | Tyrosine hydroxylase (TH) mRNA was measured in the midbrain of rats treated repeatedly with methamphetamine (MAP). Male Sprague-Dawley rats were daily injected with MAP (5 mg/kg, i.p., once daily) or saline for 14 days. Progressive augmentation was observed in MAP-induced stereotyped behaviors. After one week of abstinence, the rats were decapitated and the brains were prepared for either in situ hybridization using non-radioactive cRNA probes or Northern blot analysis using
32P-labeled cDNA probes. In situ hybridization showed that the signals of TH mRNA were localized to the dopaminergic perikarya in the midbrain and were reduced in MAP-treated animals compared to the controls. Northern blot analysis revealed that the level of TH mRNA in the midbrain of MAP-treated rats was decreased by 37% compared to the controls, which was close to the statistical significance (
P = 0.053). These results indicate that the dopaminergic hyperactivity caused by repeated MAP treatment is not associated with enhanced transcription of the TH gene. |
| doi_str_mv | 10.1016/0304-3940(95)11572-4 |
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32P-labeled cDNA probes. In situ hybridization showed that the signals of TH mRNA were localized to the dopaminergic perikarya in the midbrain and were reduced in MAP-treated animals compared to the controls. Northern blot analysis revealed that the level of TH mRNA in the midbrain of MAP-treated rats was decreased by 37% compared to the controls, which was close to the statistical significance (
P = 0.053). These results indicate that the dopaminergic hyperactivity caused by repeated MAP treatment is not associated with enhanced transcription of the TH gene.</description><identifier>ISSN: 0304-3940</identifier><identifier>EISSN: 1872-7972</identifier><identifier>DOI: 10.1016/0304-3940(95)11572-4</identifier><identifier>PMID: 7659275</identifier><identifier>CODEN: NELED5</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Animals ; Behavioral sensitization ; Biological and medical sciences ; Blotting, Northern ; Dopamine - physiology ; Dopamine neuron ; In Situ Hybridization ; Male ; Medical sciences ; Mesencephalon - drug effects ; Mesencephalon - enzymology ; Methamphetamine ; Methamphetamine - pharmacology ; Midbrain ; Motor Activity - drug effects ; Neuropharmacology ; Northern blot analysis ; Pharmacology. Drug treatments ; Psychoanaleptics: cns stimulant, antidepressant agent, nootropic agent, mood stabilizer..., (alzheimer disease) ; Psychology. Psychoanalysis. Psychiatry ; Psychopharmacology ; Rats ; Rats, Sprague-Dawley ; RNA, Messenger - biosynthesis ; Stereotyped Behavior - drug effects ; Tyrosine 3-Monooxygenase - biosynthesis ; Tyrosine hydroxylase mRNA</subject><ispartof>Neuroscience letters, 1995-05, Vol.191 (1), p.107-110</ispartof><rights>1995</rights><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c332t-f80021a6819b57814eff3329555008d700089379d1c750119ee37bb0becc4af43</citedby><cites>FETCH-LOGICAL-c332t-f80021a6819b57814eff3329555008d700089379d1c750119ee37bb0becc4af43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0304-3940(95)11572-4$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3526349$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7659275$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ishimaru, Masahiko</creatorcontrib><creatorcontrib>Hashimoto, Takanori</creatorcontrib><creatorcontrib>Obata, Kunihiko</creatorcontrib><creatorcontrib>Fukamauchi, Fumihiko</creatorcontrib><title>Methamphetamine-induced dopaminergic hyperactivity is not accompanied with increase in tyrosine hydroxylase mRNA of the rat midbrain</title><title>Neuroscience letters</title><addtitle>Neurosci Lett</addtitle><description>Tyrosine hydroxylase (TH) mRNA was measured in the midbrain of rats treated repeatedly with methamphetamine (MAP). Male Sprague-Dawley rats were daily injected with MAP (5 mg/kg, i.p., once daily) or saline for 14 days. Progressive augmentation was observed in MAP-induced stereotyped behaviors. After one week of abstinence, the rats were decapitated and the brains were prepared for either in situ hybridization using non-radioactive cRNA probes or Northern blot analysis using
32P-labeled cDNA probes. In situ hybridization showed that the signals of TH mRNA were localized to the dopaminergic perikarya in the midbrain and were reduced in MAP-treated animals compared to the controls. Northern blot analysis revealed that the level of TH mRNA in the midbrain of MAP-treated rats was decreased by 37% compared to the controls, which was close to the statistical significance (
P = 0.053). These results indicate that the dopaminergic hyperactivity caused by repeated MAP treatment is not associated with enhanced transcription of the TH gene.</description><subject>Animals</subject><subject>Behavioral sensitization</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern</subject><subject>Dopamine - physiology</subject><subject>Dopamine neuron</subject><subject>In Situ Hybridization</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Mesencephalon - drug effects</subject><subject>Mesencephalon - enzymology</subject><subject>Methamphetamine</subject><subject>Methamphetamine - pharmacology</subject><subject>Midbrain</subject><subject>Motor Activity - drug effects</subject><subject>Neuropharmacology</subject><subject>Northern blot analysis</subject><subject>Pharmacology. Drug treatments</subject><subject>Psychoanaleptics: cns stimulant, antidepressant agent, nootropic agent, mood stabilizer..., (alzheimer disease)</subject><subject>Psychology. Psychoanalysis. Psychiatry</subject><subject>Psychopharmacology</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>RNA, Messenger - biosynthesis</subject><subject>Stereotyped Behavior - drug effects</subject><subject>Tyrosine 3-Monooxygenase - biosynthesis</subject><subject>Tyrosine hydroxylase mRNA</subject><issn>0304-3940</issn><issn>1872-7972</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU-P1SAUxYnRjG9Gv4EmLIwZF1UoUMrGZDLxXzJqYnRNKL21mBYq8Ea794NL5728pa64ued3bu7lIPSEkpeU0OYVYYRXTHFyqcQLSoWsK34P7WhbCqlkfR_tTshDdJ7SD0KIoIKfoTPZCFVLsUN_PkIezbyMkM3sPFTO93sLPe7DcteI353F47pANDa7W5dX7BL2IWNjbZgX412hf7k8YudtBJOgFDivMaRiL9Y-ht_rtPXnL5-ucBhwHgFHk_Hs-i4a5x-hB4OZEjw-vhfo29s3X6_fVzef3324vrqpLGN1roaWkJqapqWqE7KlHIahCEoIQUjby3Jeq5hUPbVSEEoVAJNdRzqwlpuBswv0_DB3ieHnHlLWs0sWpsl4CPukpeRt3TTyvyBtWkVqsYH8ANpyboow6CW62cRVU6K3lPQWgd4i0Erou5T0tsjT4_x9N0N_Mh1jKfqzo26SNdMQjbcunTAm6oZxVbDXBwzKp906iDpZB77E5yLYrPvg_r3HX_30r2Q</recordid><startdate>19950519</startdate><enddate>19950519</enddate><creator>Ishimaru, Masahiko</creator><creator>Hashimoto, Takanori</creator><creator>Obata, Kunihiko</creator><creator>Fukamauchi, Fumihiko</creator><general>Elsevier Ireland Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>19950519</creationdate><title>Methamphetamine-induced dopaminergic hyperactivity is not accompanied with increase in tyrosine hydroxylase mRNA of the rat midbrain</title><author>Ishimaru, Masahiko ; Hashimoto, Takanori ; Obata, Kunihiko ; Fukamauchi, Fumihiko</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c332t-f80021a6819b57814eff3329555008d700089379d1c750119ee37bb0becc4af43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Animals</topic><topic>Behavioral sensitization</topic><topic>Biological and medical sciences</topic><topic>Blotting, Northern</topic><topic>Dopamine - physiology</topic><topic>Dopamine neuron</topic><topic>In Situ Hybridization</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Mesencephalon - drug effects</topic><topic>Mesencephalon - enzymology</topic><topic>Methamphetamine</topic><topic>Methamphetamine - pharmacology</topic><topic>Midbrain</topic><topic>Motor Activity - drug effects</topic><topic>Neuropharmacology</topic><topic>Northern blot analysis</topic><topic>Pharmacology. Drug treatments</topic><topic>Psychoanaleptics: cns stimulant, antidepressant agent, nootropic agent, mood stabilizer..., (alzheimer disease)</topic><topic>Psychology. Psychoanalysis. Psychiatry</topic><topic>Psychopharmacology</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>RNA, Messenger - biosynthesis</topic><topic>Stereotyped Behavior - drug effects</topic><topic>Tyrosine 3-Monooxygenase - biosynthesis</topic><topic>Tyrosine hydroxylase mRNA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ishimaru, Masahiko</creatorcontrib><creatorcontrib>Hashimoto, Takanori</creatorcontrib><creatorcontrib>Obata, Kunihiko</creatorcontrib><creatorcontrib>Fukamauchi, Fumihiko</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Neuroscience letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ishimaru, Masahiko</au><au>Hashimoto, Takanori</au><au>Obata, Kunihiko</au><au>Fukamauchi, Fumihiko</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Methamphetamine-induced dopaminergic hyperactivity is not accompanied with increase in tyrosine hydroxylase mRNA of the rat midbrain</atitle><jtitle>Neuroscience letters</jtitle><addtitle>Neurosci Lett</addtitle><date>1995-05-19</date><risdate>1995</risdate><volume>191</volume><issue>1</issue><spage>107</spage><epage>110</epage><pages>107-110</pages><issn>0304-3940</issn><eissn>1872-7972</eissn><coden>NELED5</coden><abstract>Tyrosine hydroxylase (TH) mRNA was measured in the midbrain of rats treated repeatedly with methamphetamine (MAP). Male Sprague-Dawley rats were daily injected with MAP (5 mg/kg, i.p., once daily) or saline for 14 days. Progressive augmentation was observed in MAP-induced stereotyped behaviors. After one week of abstinence, the rats were decapitated and the brains were prepared for either in situ hybridization using non-radioactive cRNA probes or Northern blot analysis using
32P-labeled cDNA probes. In situ hybridization showed that the signals of TH mRNA were localized to the dopaminergic perikarya in the midbrain and were reduced in MAP-treated animals compared to the controls. Northern blot analysis revealed that the level of TH mRNA in the midbrain of MAP-treated rats was decreased by 37% compared to the controls, which was close to the statistical significance (
P = 0.053). These results indicate that the dopaminergic hyperactivity caused by repeated MAP treatment is not associated with enhanced transcription of the TH gene.</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><pmid>7659275</pmid><doi>10.1016/0304-3940(95)11572-4</doi><tpages>4</tpages></addata></record> |
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| subjects | Animals Behavioral sensitization Biological and medical sciences Blotting, Northern Dopamine - physiology Dopamine neuron In Situ Hybridization Male Medical sciences Mesencephalon - drug effects Mesencephalon - enzymology Methamphetamine Methamphetamine - pharmacology Midbrain Motor Activity - drug effects Neuropharmacology Northern blot analysis Pharmacology. Drug treatments Psychoanaleptics: cns stimulant, antidepressant agent, nootropic agent, mood stabilizer..., (alzheimer disease) Psychology. Psychoanalysis. Psychiatry Psychopharmacology Rats Rats, Sprague-Dawley RNA, Messenger - biosynthesis Stereotyped Behavior - drug effects Tyrosine 3-Monooxygenase - biosynthesis Tyrosine hydroxylase mRNA |
| title | Methamphetamine-induced dopaminergic hyperactivity is not accompanied with increase in tyrosine hydroxylase mRNA of the rat midbrain |
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