Hen vitellogenin genes: A study of the distribution of phosvitin phosphoprotein coding sequences using mRNA hybridization with synthetic complementary oligonucleotide probes
1. 1. Pentadecamer DNA probes were synthesized, having complementary codons for selected unique pentapeptide sequences of low codon degeneracy present in (a) hen phosvitin minor phosphoprotein, (b) hen phosvitin major phosphoprotein, (c) both phosvitin phosphoproteins. These probes were 5′- 32P-labe...
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| Veröffentlicht in: | International journal of biochemistry 1987, Vol.19 (1), p.33-39 |
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| container_title | International journal of biochemistry |
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| creator | Wicks, Richard J. Clark, Richard C. |
| description | 1.
1. Pentadecamer DNA probes were synthesized, having complementary codons for selected unique pentapeptide sequences of low codon degeneracy present in (a) hen phosvitin minor phosphoprotein, (b) hen phosvitin major phosphoprotein, (c) both phosvitin phosphoproteins. These probes were 5′-
32P-labelled.
2.
2. Vitellogenin mRNA was isolated from estrogenized chick liver, fractionated by electrophoresis using formaldehyde/agarose gels and blot transferred to nitrocellulose paper.
3.
3. Relative yields of the two vitellogenin mRNAs differed with the extraction method used.
4.
4. The minor phosphoprotein DNA probe formed a hybrid with a 1.6 megadalton component. The remaining two probes hybridized to a 2.3 megadalton component, this being the expected size of a full-length message. The smallest polyadenylated fragment to which the major phosphoprotein DNA probe hybridized was 1.0 megadalton. The remaining two probes hybridized to fragments of 0.7 megadalton and possibly smaller.
5.
5. Phosvitin major phosphoprotein is concluded to be coded for by part of the larger vitellogenin mRNA, while the minor phosphoprotein is coded for by part of the smaller vitellogenin mRNA. Estimates of the distances of the hybridization sites from polyadenylated tails are also given. |
| doi_str_mv | 10.1016/0020-711X(87)90120-0 |
| format | Article |
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1. Pentadecamer DNA probes were synthesized, having complementary codons for selected unique pentapeptide sequences of low codon degeneracy present in (a) hen phosvitin minor phosphoprotein, (b) hen phosvitin major phosphoprotein, (c) both phosvitin phosphoproteins. These probes were 5′-
32P-labelled.
2.
2. Vitellogenin mRNA was isolated from estrogenized chick liver, fractionated by electrophoresis using formaldehyde/agarose gels and blot transferred to nitrocellulose paper.
3.
3. Relative yields of the two vitellogenin mRNAs differed with the extraction method used.
4.
4. The minor phosphoprotein DNA probe formed a hybrid with a 1.6 megadalton component. The remaining two probes hybridized to a 2.3 megadalton component, this being the expected size of a full-length message. The smallest polyadenylated fragment to which the major phosphoprotein DNA probe hybridized was 1.0 megadalton. The remaining two probes hybridized to fragments of 0.7 megadalton and possibly smaller.
5.
5. Phosvitin major phosphoprotein is concluded to be coded for by part of the larger vitellogenin mRNA, while the minor phosphoprotein is coded for by part of the smaller vitellogenin mRNA. Estimates of the distances of the hybridization sites from polyadenylated tails are also given.</description><identifier>ISSN: 0020-711X</identifier><identifier>DOI: 10.1016/0020-711X(87)90120-0</identifier><identifier>PMID: 3569638</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Animals ; Chickens ; DNA - genetics ; Egg Proteins - genetics ; Female ; genetic code ; molecular genetics ; Molecular Weight ; Nucleic Acid Hybridization ; Oligodeoxyribonucleotides - chemical synthesis ; Oligodeoxyribonucleotides - genetics ; Peptide Fragments - genetics ; Phosphoproteins - genetics ; Phosvitin - genetics ; proteins ; RNA ; RNA, Messenger - genetics ; roosters ; Vitellogenins - genetics</subject><ispartof>International journal of biochemistry, 1987, Vol.19 (1), p.33-39</ispartof><rights>1987</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c276t-b73b04915cef6c968d45e98b0ae6e4251c6fffaee314c3736986cab006a81a3c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,4010,27904,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3569638$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wicks, Richard J.</creatorcontrib><creatorcontrib>Clark, Richard C.</creatorcontrib><title>Hen vitellogenin genes: A study of the distribution of phosvitin phosphoprotein coding sequences using mRNA hybridization with synthetic complementary oligonucleotide probes</title><title>International journal of biochemistry</title><addtitle>Int J Biochem</addtitle><description>1.
1. Pentadecamer DNA probes were synthesized, having complementary codons for selected unique pentapeptide sequences of low codon degeneracy present in (a) hen phosvitin minor phosphoprotein, (b) hen phosvitin major phosphoprotein, (c) both phosvitin phosphoproteins. These probes were 5′-
32P-labelled.
2.
2. Vitellogenin mRNA was isolated from estrogenized chick liver, fractionated by electrophoresis using formaldehyde/agarose gels and blot transferred to nitrocellulose paper.
3.
3. Relative yields of the two vitellogenin mRNAs differed with the extraction method used.
4.
4. The minor phosphoprotein DNA probe formed a hybrid with a 1.6 megadalton component. The remaining two probes hybridized to a 2.3 megadalton component, this being the expected size of a full-length message. The smallest polyadenylated fragment to which the major phosphoprotein DNA probe hybridized was 1.0 megadalton. The remaining two probes hybridized to fragments of 0.7 megadalton and possibly smaller.
5.
5. Phosvitin major phosphoprotein is concluded to be coded for by part of the larger vitellogenin mRNA, while the minor phosphoprotein is coded for by part of the smaller vitellogenin mRNA. Estimates of the distances of the hybridization sites from polyadenylated tails are also given.</description><subject>Animals</subject><subject>Chickens</subject><subject>DNA - genetics</subject><subject>Egg Proteins - genetics</subject><subject>Female</subject><subject>genetic code</subject><subject>molecular genetics</subject><subject>Molecular Weight</subject><subject>Nucleic Acid Hybridization</subject><subject>Oligodeoxyribonucleotides - chemical synthesis</subject><subject>Oligodeoxyribonucleotides - genetics</subject><subject>Peptide Fragments - genetics</subject><subject>Phosphoproteins - genetics</subject><subject>Phosvitin - genetics</subject><subject>proteins</subject><subject>RNA</subject><subject>RNA, Messenger - genetics</subject><subject>roosters</subject><subject>Vitellogenins - genetics</subject><issn>0020-711X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUU1v1DAQzQFUSuEfgPAJwWHBEyd2wgFpVVGKVIEEVOJmOc5k1yixF9spWv4T_7GT7qpHOPhjxu-98cwrimfA3wAH-Zbzkq8UwI9XjXrdcqCIPyhO79OPiscp_eQc2qaCk-JE1LKVojkt_l6iZzcu4ziGDXrnGe2Y3rE1S3nu9ywMLG-R9S7l6Lo5u-CX3G4bEtEIv9xo7WLISKENvfMblvDXjN5iYnNa4unr5zXb7rvoevfH3Kn8dnnL0t6TfHaWiNNuxAl9NpHKjm4T_GxHDNn1yEi-w_SkeDiYMeHT43lWXF98-H5-ubr68vHT-fpqZUsl86pTouNVC7XFQdpWNn1VY9t03KDEqqzBymEYDKKAygolZNtIazrOpWnACCvOipcHXSpLfaSsJ5cszch4DHPSSlUNKFX_FwiEq0TZErA6AG0MKUUc9C66iTrVwPVioV680otXulH6zkLNifb8qD93E_b3pKN_9P7i8D6YoM0muqSvv5UcBIcKWglAiPcHBNK8bhxGnaxbnOldRJt1H9y_v3AL9Ve8MQ</recordid><startdate>1987</startdate><enddate>1987</enddate><creator>Wicks, Richard J.</creator><creator>Clark, Richard C.</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>1987</creationdate><title>Hen vitellogenin genes: A study of the distribution of phosvitin phosphoprotein coding sequences using mRNA hybridization with synthetic complementary oligonucleotide probes</title><author>Wicks, Richard J. ; Clark, Richard C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c276t-b73b04915cef6c968d45e98b0ae6e4251c6fffaee314c3736986cab006a81a3c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Animals</topic><topic>Chickens</topic><topic>DNA - genetics</topic><topic>Egg Proteins - genetics</topic><topic>Female</topic><topic>genetic code</topic><topic>molecular genetics</topic><topic>Molecular Weight</topic><topic>Nucleic Acid Hybridization</topic><topic>Oligodeoxyribonucleotides - chemical synthesis</topic><topic>Oligodeoxyribonucleotides - genetics</topic><topic>Peptide Fragments - genetics</topic><topic>Phosphoproteins - genetics</topic><topic>Phosvitin - genetics</topic><topic>proteins</topic><topic>RNA</topic><topic>RNA, Messenger - genetics</topic><topic>roosters</topic><topic>Vitellogenins - genetics</topic><toplevel>online_resources</toplevel><creatorcontrib>Wicks, Richard J.</creatorcontrib><creatorcontrib>Clark, Richard C.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wicks, Richard J.</au><au>Clark, Richard C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hen vitellogenin genes: A study of the distribution of phosvitin phosphoprotein coding sequences using mRNA hybridization with synthetic complementary oligonucleotide probes</atitle><jtitle>International journal of biochemistry</jtitle><addtitle>Int J Biochem</addtitle><date>1987</date><risdate>1987</risdate><volume>19</volume><issue>1</issue><spage>33</spage><epage>39</epage><pages>33-39</pages><issn>0020-711X</issn><abstract>1.
1. Pentadecamer DNA probes were synthesized, having complementary codons for selected unique pentapeptide sequences of low codon degeneracy present in (a) hen phosvitin minor phosphoprotein, (b) hen phosvitin major phosphoprotein, (c) both phosvitin phosphoproteins. These probes were 5′-
32P-labelled.
2.
2. Vitellogenin mRNA was isolated from estrogenized chick liver, fractionated by electrophoresis using formaldehyde/agarose gels and blot transferred to nitrocellulose paper.
3.
3. Relative yields of the two vitellogenin mRNAs differed with the extraction method used.
4.
4. The minor phosphoprotein DNA probe formed a hybrid with a 1.6 megadalton component. The remaining two probes hybridized to a 2.3 megadalton component, this being the expected size of a full-length message. The smallest polyadenylated fragment to which the major phosphoprotein DNA probe hybridized was 1.0 megadalton. The remaining two probes hybridized to fragments of 0.7 megadalton and possibly smaller.
5.
5. Phosvitin major phosphoprotein is concluded to be coded for by part of the larger vitellogenin mRNA, while the minor phosphoprotein is coded for by part of the smaller vitellogenin mRNA. Estimates of the distances of the hybridization sites from polyadenylated tails are also given.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>3569638</pmid><doi>10.1016/0020-711X(87)90120-0</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | International journal of biochemistry, 1987, Vol.19 (1), p.33-39 |
| issn | 0020-711X |
| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Chickens DNA - genetics Egg Proteins - genetics Female genetic code molecular genetics Molecular Weight Nucleic Acid Hybridization Oligodeoxyribonucleotides - chemical synthesis Oligodeoxyribonucleotides - genetics Peptide Fragments - genetics Phosphoproteins - genetics Phosvitin - genetics proteins RNA RNA, Messenger - genetics roosters Vitellogenins - genetics |
| title | Hen vitellogenin genes: A study of the distribution of phosvitin phosphoprotein coding sequences using mRNA hybridization with synthetic complementary oligonucleotide probes |
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