Effect of recombinant human tumor necrosis factor on the induction of murine macrophage tumoricidal activity
The ability of recombinant human tumor necrosis factor (rH-TNF) alone or in combination with lymphokines (LK) to induce the in vitro activation of murine macrophages was evaluated. The treatment of C57BL/6 mouse resident peritoneal exudate cells (PEC) with rH-TNF and LK was found to induce the activ...
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| Veröffentlicht in: | Cancer research (Chicago, Ill.) Ill.), 1987-06, Vol.47 (11), p.2793-2798 |
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| container_title | Cancer research (Chicago, Ill.) |
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| creator | HORI, K EHRKE, M. J MACE, K MACCUBIN, D DOYLE, M. J OTSUKA, Y MIHICH, E |
| description | The ability of recombinant human tumor necrosis factor (rH-TNF) alone or in combination with lymphokines (LK) to induce the in vitro activation of murine macrophages was evaluated. The treatment of C57BL/6 mouse resident peritoneal exudate cells (PEC) with rH-TNF and LK was found to induce the activation of macrophages to a tumoricidal state against P815 mastocytoma cells. Neither rH-TNF nor LK alone induced macrophage cytotoxic activity. Furthermore, the macrophage activation seen was not due to small amounts of contaminating lipopolysaccharide. The TNF plus LK-mediated macrophage activation could be totally ablated by rabbit antiserum to murine gamma-interferon, thus suggesting a role for gamma-interferon in this system. Since adherent cells (greater than or equal to 95% macrophages) only marginally responded to stimulation with rH-TNF plus LK and the addition of nonadherent PEC caused a marked augmentation of rH-TNF plus LK-mediated macrophage activation, the involvement of nonadherent PEC was suggested. In addition, using antibodies and complement to deplete subsets of cells from the nonadherent PEC, the requirement for cells bearing Thy 1.2 and asialo GM1 surface markers was demonstrated. These results suggest that TNF may play an autocrine regulatory role in concert with lymphokines in macrophage-mediated host defense against malignant neoplasia. |
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J ; MACE, K ; MACCUBIN, D ; DOYLE, M. J ; OTSUKA, Y ; MIHICH, E</creator><creatorcontrib>HORI, K ; EHRKE, M. J ; MACE, K ; MACCUBIN, D ; DOYLE, M. J ; OTSUKA, Y ; MIHICH, E</creatorcontrib><description>The ability of recombinant human tumor necrosis factor (rH-TNF) alone or in combination with lymphokines (LK) to induce the in vitro activation of murine macrophages was evaluated. The treatment of C57BL/6 mouse resident peritoneal exudate cells (PEC) with rH-TNF and LK was found to induce the activation of macrophages to a tumoricidal state against P815 mastocytoma cells. Neither rH-TNF nor LK alone induced macrophage cytotoxic activity. Furthermore, the macrophage activation seen was not due to small amounts of contaminating lipopolysaccharide. The TNF plus LK-mediated macrophage activation could be totally ablated by rabbit antiserum to murine gamma-interferon, thus suggesting a role for gamma-interferon in this system. Since adherent cells (greater than or equal to 95% macrophages) only marginally responded to stimulation with rH-TNF plus LK and the addition of nonadherent PEC caused a marked augmentation of rH-TNF plus LK-mediated macrophage activation, the involvement of nonadherent PEC was suggested. In addition, using antibodies and complement to deplete subsets of cells from the nonadherent PEC, the requirement for cells bearing Thy 1.2 and asialo GM1 surface markers was demonstrated. These results suggest that TNF may play an autocrine regulatory role in concert with lymphokines in macrophage-mediated host defense against malignant neoplasia.</description><identifier>ISSN: 0008-5472</identifier><identifier>EISSN: 1538-7445</identifier><identifier>PMID: 2882835</identifier><identifier>CODEN: CNREA8</identifier><language>eng</language><publisher>Philadelphia, PA: American Association for Cancer Research</publisher><subject>Animals ; Antigens, Surface - analysis ; Biological and medical sciences ; Cells, Cultured ; Cytotoxicity, Immunologic ; Dose-Response Relationship, Drug ; G(M1) Ganglioside ; Glycoproteins - pharmacology ; Glycosphingolipids - analysis ; Humans ; Interferon-gamma - physiology ; Lipopolysaccharides - pharmacology ; Lymphokines - pharmacology ; Macrophage Activation - drug effects ; Macrophages - drug effects ; Macrophages - immunology ; Medical sciences ; Mice ; Neoplasms, Experimental - immunology ; Other treatments ; Peritoneal Cavity - cytology ; Recombinant Proteins - pharmacology ; Thy-1 Antigens ; Time Factors ; Treatment. General aspects ; Tumor Necrosis Factor-alpha ; Tumors</subject><ispartof>Cancer research (Chicago, Ill.), 1987-06, Vol.47 (11), p.2793-2798</ispartof><rights>1988 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7384550$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2882835$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>HORI, K</creatorcontrib><creatorcontrib>EHRKE, M. J</creatorcontrib><creatorcontrib>MACE, K</creatorcontrib><creatorcontrib>MACCUBIN, D</creatorcontrib><creatorcontrib>DOYLE, M. J</creatorcontrib><creatorcontrib>OTSUKA, Y</creatorcontrib><creatorcontrib>MIHICH, E</creatorcontrib><title>Effect of recombinant human tumor necrosis factor on the induction of murine macrophage tumoricidal activity</title><title>Cancer research (Chicago, Ill.)</title><addtitle>Cancer Res</addtitle><description>The ability of recombinant human tumor necrosis factor (rH-TNF) alone or in combination with lymphokines (LK) to induce the in vitro activation of murine macrophages was evaluated. The treatment of C57BL/6 mouse resident peritoneal exudate cells (PEC) with rH-TNF and LK was found to induce the activation of macrophages to a tumoricidal state against P815 mastocytoma cells. Neither rH-TNF nor LK alone induced macrophage cytotoxic activity. Furthermore, the macrophage activation seen was not due to small amounts of contaminating lipopolysaccharide. The TNF plus LK-mediated macrophage activation could be totally ablated by rabbit antiserum to murine gamma-interferon, thus suggesting a role for gamma-interferon in this system. Since adherent cells (greater than or equal to 95% macrophages) only marginally responded to stimulation with rH-TNF plus LK and the addition of nonadherent PEC caused a marked augmentation of rH-TNF plus LK-mediated macrophage activation, the involvement of nonadherent PEC was suggested. In addition, using antibodies and complement to deplete subsets of cells from the nonadherent PEC, the requirement for cells bearing Thy 1.2 and asialo GM1 surface markers was demonstrated. These results suggest that TNF may play an autocrine regulatory role in concert with lymphokines in macrophage-mediated host defense against malignant neoplasia.</description><subject>Animals</subject><subject>Antigens, Surface - analysis</subject><subject>Biological and medical sciences</subject><subject>Cells, Cultured</subject><subject>Cytotoxicity, Immunologic</subject><subject>Dose-Response Relationship, Drug</subject><subject>G(M1) Ganglioside</subject><subject>Glycoproteins - pharmacology</subject><subject>Glycosphingolipids - analysis</subject><subject>Humans</subject><subject>Interferon-gamma - physiology</subject><subject>Lipopolysaccharides - pharmacology</subject><subject>Lymphokines - pharmacology</subject><subject>Macrophage Activation - drug effects</subject><subject>Macrophages - drug effects</subject><subject>Macrophages - immunology</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Neoplasms, Experimental - immunology</subject><subject>Other treatments</subject><subject>Peritoneal Cavity - cytology</subject><subject>Recombinant Proteins - pharmacology</subject><subject>Thy-1 Antigens</subject><subject>Time Factors</subject><subject>Treatment. General aspects</subject><subject>Tumor Necrosis Factor-alpha</subject><subject>Tumors</subject><issn>0008-5472</issn><issn>1538-7445</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kE1LAzEQhoMotVZ_gpCDeFvIbjZNepRSP6DgRc9LNpmxkU22Jlmh_95AF0_DM-_zzmEuyLIWXFWybcUlWTLGVCVa2VyTm5S-C4qaiQVZNEo1ioslGXaIYDIdkUYwo-9d0CHTw-R1oHnyY6QBTByTSxS1yYXHEhyAumAnk12h0vVTdAGo10U9HvQXnLvOOKsHWnru1-XTLblCPSS4m-eKfD7vPrav1f795W37tK8OzXqTK-QNt0oq2bdC1doiiLUxCmUvpLLIDDYCERmuW1ZveuiBc6Eba8pKghV8RR7Pd49x_Jkg5c67ZGAYdIBxSp2UrdxIJot4P4tT78F2x-i8jqdufk_JH-ZcJ6MHjDoYl_41yVUrBON_7glx-Q</recordid><startdate>19870601</startdate><enddate>19870601</enddate><creator>HORI, K</creator><creator>EHRKE, M. J</creator><creator>MACE, K</creator><creator>MACCUBIN, D</creator><creator>DOYLE, M. J</creator><creator>OTSUKA, Y</creator><creator>MIHICH, E</creator><general>American Association for Cancer Research</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19870601</creationdate><title>Effect of recombinant human tumor necrosis factor on the induction of murine macrophage tumoricidal activity</title><author>HORI, K ; EHRKE, M. J ; MACE, K ; MACCUBIN, D ; DOYLE, M. J ; OTSUKA, Y ; MIHICH, E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h269t-f323d8787b4581adfe56cc8f7b578df0cf25fff0f64019bebe335a2dcf0f7ed53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Animals</topic><topic>Antigens, Surface - analysis</topic><topic>Biological and medical sciences</topic><topic>Cells, Cultured</topic><topic>Cytotoxicity, Immunologic</topic><topic>Dose-Response Relationship, Drug</topic><topic>G(M1) Ganglioside</topic><topic>Glycoproteins - pharmacology</topic><topic>Glycosphingolipids - analysis</topic><topic>Humans</topic><topic>Interferon-gamma - physiology</topic><topic>Lipopolysaccharides - pharmacology</topic><topic>Lymphokines - pharmacology</topic><topic>Macrophage Activation - drug effects</topic><topic>Macrophages - drug effects</topic><topic>Macrophages - immunology</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Neoplasms, Experimental - immunology</topic><topic>Other treatments</topic><topic>Peritoneal Cavity - cytology</topic><topic>Recombinant Proteins - pharmacology</topic><topic>Thy-1 Antigens</topic><topic>Time Factors</topic><topic>Treatment. General aspects</topic><topic>Tumor Necrosis Factor-alpha</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>HORI, K</creatorcontrib><creatorcontrib>EHRKE, M. J</creatorcontrib><creatorcontrib>MACE, K</creatorcontrib><creatorcontrib>MACCUBIN, D</creatorcontrib><creatorcontrib>DOYLE, M. J</creatorcontrib><creatorcontrib>OTSUKA, Y</creatorcontrib><creatorcontrib>MIHICH, E</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Cancer research (Chicago, Ill.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>HORI, K</au><au>EHRKE, M. J</au><au>MACE, K</au><au>MACCUBIN, D</au><au>DOYLE, M. J</au><au>OTSUKA, Y</au><au>MIHICH, E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of recombinant human tumor necrosis factor on the induction of murine macrophage tumoricidal activity</atitle><jtitle>Cancer research (Chicago, Ill.)</jtitle><addtitle>Cancer Res</addtitle><date>1987-06-01</date><risdate>1987</risdate><volume>47</volume><issue>11</issue><spage>2793</spage><epage>2798</epage><pages>2793-2798</pages><issn>0008-5472</issn><eissn>1538-7445</eissn><coden>CNREA8</coden><abstract>The ability of recombinant human tumor necrosis factor (rH-TNF) alone or in combination with lymphokines (LK) to induce the in vitro activation of murine macrophages was evaluated. The treatment of C57BL/6 mouse resident peritoneal exudate cells (PEC) with rH-TNF and LK was found to induce the activation of macrophages to a tumoricidal state against P815 mastocytoma cells. Neither rH-TNF nor LK alone induced macrophage cytotoxic activity. Furthermore, the macrophage activation seen was not due to small amounts of contaminating lipopolysaccharide. The TNF plus LK-mediated macrophage activation could be totally ablated by rabbit antiserum to murine gamma-interferon, thus suggesting a role for gamma-interferon in this system. Since adherent cells (greater than or equal to 95% macrophages) only marginally responded to stimulation with rH-TNF plus LK and the addition of nonadherent PEC caused a marked augmentation of rH-TNF plus LK-mediated macrophage activation, the involvement of nonadherent PEC was suggested. In addition, using antibodies and complement to deplete subsets of cells from the nonadherent PEC, the requirement for cells bearing Thy 1.2 and asialo GM1 surface markers was demonstrated. These results suggest that TNF may play an autocrine regulatory role in concert with lymphokines in macrophage-mediated host defense against malignant neoplasia.</abstract><cop>Philadelphia, PA</cop><pub>American Association for Cancer Research</pub><pmid>2882835</pmid><tpages>6</tpages></addata></record> |
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| ispartof | Cancer research (Chicago, Ill.), 1987-06, Vol.47 (11), p.2793-2798 |
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| source | MEDLINE; American Association for Cancer Research; EZB-FREE-00999 freely available EZB journals |
| subjects | Animals Antigens, Surface - analysis Biological and medical sciences Cells, Cultured Cytotoxicity, Immunologic Dose-Response Relationship, Drug G(M1) Ganglioside Glycoproteins - pharmacology Glycosphingolipids - analysis Humans Interferon-gamma - physiology Lipopolysaccharides - pharmacology Lymphokines - pharmacology Macrophage Activation - drug effects Macrophages - drug effects Macrophages - immunology Medical sciences Mice Neoplasms, Experimental - immunology Other treatments Peritoneal Cavity - cytology Recombinant Proteins - pharmacology Thy-1 Antigens Time Factors Treatment. General aspects Tumor Necrosis Factor-alpha Tumors |
| title | Effect of recombinant human tumor necrosis factor on the induction of murine macrophage tumoricidal activity |
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