Identification of a male-specific (H-Y) antigen on the flagellar plasma membrane of ram epididymal spermatozoa
H-Y (male-specific) antigen has been detected on the plasma membranes of both caput and caudal ram spermatozoa using both immunoperoxidase and immunofluorescence labelling techniques. In these spermatozoa the distribution of H-Y antigen appears to be confined to both the posterior region of the head...
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| Veröffentlicht in: | Human genetics 1987-04, Vol.75 (4), p.362-367 |
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| container_title | Human genetics |
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| creator | BRADLEY, M. P FORRESTER, I. T HESLOP, B. F |
| description | H-Y (male-specific) antigen has been detected on the plasma membranes of both caput and caudal ram spermatozoa using both immunoperoxidase and immunofluorescence labelling techniques. In these spermatozoa the distribution of H-Y antigen appears to be confined to both the posterior region of the head and the mid-piece region of the flagellum. In addition, caput spermatozoa also exhibit intense immunoperoxidase staining of the cytoplasmic droplet which is situated on the flagellum at the base of the head. Western blot analyses of purified plasma membranes from the flagella of caudal spermatozoa have revealed the presence of a male-specific protein with an estimated molecular weight of 25,000-27,000. |
| doi_str_mv | 10.1007/bf00284109 |
| format | Article |
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Western blot analyses of purified plasma membranes from the flagella of caudal spermatozoa have revealed the presence of a male-specific protein with an estimated molecular weight of 25,000-27,000.</description><identifier>ISSN: 0340-6717</identifier><identifier>EISSN: 1432-1203</identifier><identifier>DOI: 10.1007/bf00284109</identifier><identifier>PMID: 3552956</identifier><identifier>CODEN: HUGEDQ</identifier><language>eng</language><publisher>Heidelberg: Springer</publisher><subject>Animals ; Biological and medical sciences ; Cell Membrane - immunology ; Enzyme-Linked Immunosorbent Assay ; Epididymis ; Flagella - immunology ; Fluorescent Antibody Technique ; Fundamental and applied biological sciences. Psychology ; H-Y Antigen - analysis ; Male ; Mammalian male genital system ; Morphology. 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P</creatorcontrib><creatorcontrib>FORRESTER, I. T</creatorcontrib><creatorcontrib>HESLOP, B. F</creatorcontrib><title>Identification of a male-specific (H-Y) antigen on the flagellar plasma membrane of ram epididymal spermatozoa</title><title>Human genetics</title><addtitle>Hum Genet</addtitle><description>H-Y (male-specific) antigen has been detected on the plasma membranes of both caput and caudal ram spermatozoa using both immunoperoxidase and immunofluorescence labelling techniques. In these spermatozoa the distribution of H-Y antigen appears to be confined to both the posterior region of the head and the mid-piece region of the flagellum. In addition, caput spermatozoa also exhibit intense immunoperoxidase staining of the cytoplasmic droplet which is situated on the flagellum at the base of the head. Western blot analyses of purified plasma membranes from the flagella of caudal spermatozoa have revealed the presence of a male-specific protein with an estimated molecular weight of 25,000-27,000.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell Membrane - immunology</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Epididymis</subject><subject>Flagella - immunology</subject><subject>Fluorescent Antibody Technique</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>H-Y Antigen - analysis</subject><subject>Male</subject><subject>Mammalian male genital system</subject><subject>Morphology. Physiology</subject><subject>Sheep</subject><subject>Spermatozoa - immunology</subject><subject>Vertebrates: reproduction</subject><issn>0340-6717</issn><issn>1432-1203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kE1LAzEQhoMotVYv3oUcRFRYzSabze5Ri7WFghc9eFpmk0ld2S-T7aH-elNaexqY95mH4SXkMmYPMWPqsbSM8SyJWX5ExnEieBRzJo7JmImERamK1Sk58_6bsVjmXI7ISEjJc5mOSbsw2A6VrTQMVdfSzlKgDdQY-R71dk9v59HnHYVArTAALR2-kNoaVljX4Ghfg2_CDTalgxa3BgcNxb4yldkEFQ0m18DQ_XZwTk4s1B4v9nNCPmYv79N5tHx7XUyflpEWSg2RAVSJ1EyITEkjEs5lZo2wqRXSGJvKUmmVG8RUpjZFkUkLmVUSSsMxASsm5Gbn7V33s0Y_FE3l9fbhFru1L5RKVMZyHsD7Hahd571DW_SuasBtipgV23KL59l_uQG-2lvXZYPmgO7bDPn1PgevobahD135A5ZxxmXCxB9YuYHJ</recordid><startdate>19870401</startdate><enddate>19870401</enddate><creator>BRADLEY, M. P</creator><creator>FORRESTER, I. T</creator><creator>HESLOP, B. F</creator><general>Springer</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19870401</creationdate><title>Identification of a male-specific (H-Y) antigen on the flagellar plasma membrane of ram epididymal spermatozoa</title><author>BRADLEY, M. P ; FORRESTER, I. T ; HESLOP, B. F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c377t-dae745c033875d342258fd3f6f35ddf65b7c79dee656f6e385fa8f75abd2e4af3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell Membrane - immunology</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Epididymis</topic><topic>Flagella - immunology</topic><topic>Fluorescent Antibody Technique</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>H-Y Antigen - analysis</topic><topic>Male</topic><topic>Mammalian male genital system</topic><topic>Morphology. Physiology</topic><topic>Sheep</topic><topic>Spermatozoa - immunology</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>BRADLEY, M. P</creatorcontrib><creatorcontrib>FORRESTER, I. T</creatorcontrib><creatorcontrib>HESLOP, B. F</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Human genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>BRADLEY, M. P</au><au>FORRESTER, I. T</au><au>HESLOP, B. F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of a male-specific (H-Y) antigen on the flagellar plasma membrane of ram epididymal spermatozoa</atitle><jtitle>Human genetics</jtitle><addtitle>Hum Genet</addtitle><date>1987-04-01</date><risdate>1987</risdate><volume>75</volume><issue>4</issue><spage>362</spage><epage>367</epage><pages>362-367</pages><issn>0340-6717</issn><eissn>1432-1203</eissn><coden>HUGEDQ</coden><abstract>H-Y (male-specific) antigen has been detected on the plasma membranes of both caput and caudal ram spermatozoa using both immunoperoxidase and immunofluorescence labelling techniques. In these spermatozoa the distribution of H-Y antigen appears to be confined to both the posterior region of the head and the mid-piece region of the flagellum. In addition, caput spermatozoa also exhibit intense immunoperoxidase staining of the cytoplasmic droplet which is situated on the flagellum at the base of the head. Western blot analyses of purified plasma membranes from the flagella of caudal spermatozoa have revealed the presence of a male-specific protein with an estimated molecular weight of 25,000-27,000.</abstract><cop>Heidelberg</cop><cop>Berlin</cop><cop>New York, NY</cop><pub>Springer</pub><pmid>3552956</pmid><doi>10.1007/bf00284109</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0340-6717 |
| ispartof | Human genetics, 1987-04, Vol.75 (4), p.362-367 |
| issn | 0340-6717 1432-1203 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals |
| subjects | Animals Biological and medical sciences Cell Membrane - immunology Enzyme-Linked Immunosorbent Assay Epididymis Flagella - immunology Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology H-Y Antigen - analysis Male Mammalian male genital system Morphology. Physiology Sheep Spermatozoa - immunology Vertebrates: reproduction |
| title | Identification of a male-specific (H-Y) antigen on the flagellar plasma membrane of ram epididymal spermatozoa |
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