Tracking movements of lipids and Thy1 molecules in the plasmalemma of living fibroblasts by fluorescence video microscopy with nanometer scale precision

The lateral diffusion of 100 nm fluorescent latex microspheres (FS) bound to either N-biotinyl-phosphatidyl-ethanolamine or the glycosylphosphatidylinositol-linked protein Thy1 were monitored in the plasmalemma of primary rat fibroblasts by single particle tracking of FS centroids from digital fluor...

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Veröffentlicht in:	The Journal of membrane biology 1995-04, Vol.144 (3), p.231-244
Hauptverfasser:	Hicks, B W, Angelides, K J
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biotin - metabolism Cell Membrane - metabolism Cells, Cultured Diffusion Fibroblasts - metabolism Fibroblasts - ultrastructure Fluorescent Dyes - metabolism Image Processing, Computer-Assisted Membrane Fluidity Microscopy, Fluorescence Microscopy, Video Phosphatidylethanolamines - metabolism Rats Thy-1 Antigens - immunology Thy-1 Antigens - metabolism Time Factors
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