Comparison of Interleukin-1α Gene Expression and Protein Levels in the Murine Spleen after Lethal and Sublethal Total-Body Irradiation
To understand the effects of ionizing radiation on the production of IL-1α in vivo within a hematopoietic organ, we evaluated acute changes in splenic IL-1α mRNA and IL-1α protein after exposing ${\rm B}6{\rm D}2{\rm F}_{1}$ mice to lethal and sublethal 60 Co radiation. Results suggest that in vivo,...
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| Veröffentlicht in: | Radiation research 1995-09, Vol.143 (3), p.320-326 |
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| container_title | Radiation research |
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| creator | Baker, William H. Limanni, Alex Chang, Cheng-min Jackson, William E. Seemann, Ruth Patchen, Myra L. |
| description | To understand the effects of ionizing radiation on the production of IL-1α in vivo within a hematopoietic organ, we evaluated acute changes in splenic IL-1α mRNA and IL-1α protein after exposing ${\rm B}6{\rm D}2{\rm F}_{1}$ mice to lethal and sublethal 60 Co radiation. Results suggest that in vivo, ionizing radiation induces a time- and dose-dependent accumulation of IL-1α mRNA in the mouse spleen after exposure to γ radiation. Time-dependent increases in the level of IL-1α protein were also observed, although the magnitude of increased protein expression did not complement the magnitude of the accumulation of the message. Selective concentration of cells producing IL-1α does not appear to account completely for the increase in splenic IL-1α mRNA observed in this in vivo system. |
| doi_str_mv | 10.2307/3579220 |
| format | Article |
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Results suggest that in vivo, ionizing radiation induces a time- and dose-dependent accumulation of IL-1α mRNA in the mouse spleen after exposure to γ radiation. Time-dependent increases in the level of IL-1α protein were also observed, although the magnitude of increased protein expression did not complement the magnitude of the accumulation of the message. Selective concentration of cells producing IL-1α does not appear to account completely for the increase in splenic IL-1α mRNA observed in this in vivo system.</description><identifier>ISSN: 0033-7587</identifier><identifier>EISSN: 1938-5404</identifier><identifier>DOI: 10.2307/3579220</identifier><identifier>PMID: 7652171</identifier><identifier>CODEN: RAREAE</identifier><language>eng</language><publisher>Oak Brook, Il: Radiation Research Society</publisher><subject>Animals ; Base Sequence ; Biological and medical sciences ; Biological effects of radiation ; Female ; Fundamental and applied biological sciences. 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Results suggest that in vivo, ionizing radiation induces a time- and dose-dependent accumulation of IL-1α mRNA in the mouse spleen after exposure to γ radiation. Time-dependent increases in the level of IL-1α protein were also observed, although the magnitude of increased protein expression did not complement the magnitude of the accumulation of the message. Selective concentration of cells producing IL-1α does not appear to account completely for the increase in splenic IL-1α mRNA observed in this in vivo system.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Biological effects of radiation</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>Gene Expression - radiation effects</subject><subject>Interleukin-1 - analysis</subject><subject>Interleukin-1 - genetics</subject><subject>Ionizing radiation</subject><subject>Ionizing radiations</subject><subject>Irradiation</subject><subject>Lymphocytes</subject><subject>Messenger RNA</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>Radiation dosage</subject><subject>RNA</subject><subject>RNA, Messenger - analysis</subject><subject>Space life sciences</subject><subject>Spleen</subject><subject>Spleen - metabolism</subject><subject>Spleen cells</subject><subject>Tissues, organs and organisms biophysics</subject><subject>Whole-Body Irradiation</subject><issn>0033-7587</issn><issn>1938-5404</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kc1u1DAQxy0EarcF8QRIPiB6Co2_kyOs-rHSIiq1nCPHnqhuvXGwHUSfgOfhRXimumxUTpxmRvOb_2j-g9BbUn-krFanTKiW0voFWpGWNZXgNX-JVnXNWKVEow7RUUp3damJbA_QgZKCEkVW6Nc67CYdXQojDgPejBmih_nejRX58xtfwAj47OcUISVXED1afBVDBjfiLfwAn3DJ8i3gL3N0hb2ePEDhhqJTiHyr_d-h67n3--omZO2rz8E-4E2M2jqdi_Jr9GrQPsGbJR6jb-dnN-vLavv1YrP-tK0MYzxXpBno0FIrTcuBS8MpkyWxxIDiUkuhjLRcN0LZBhoGlFgqet7zpqdCEMOO0Ye97hTD9xlS7nYuGfBejxDm1CnFlSzWFPBkD5oYUoowdFN0Ox0fOlJ3T5Z3i-WFfLdIzv0O7DO3eFz675e-Tkb7IerRuPSMlQPKx_g_7C7lEP-77RFi6ZQ3</recordid><startdate>19950901</startdate><enddate>19950901</enddate><creator>Baker, William H.</creator><creator>Limanni, Alex</creator><creator>Chang, Cheng-min</creator><creator>Jackson, William E.</creator><creator>Seemann, Ruth</creator><creator>Patchen, Myra L.</creator><general>Radiation Research Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19950901</creationdate><title>Comparison of Interleukin-1α Gene Expression and Protein Levels in the Murine Spleen after Lethal and Sublethal Total-Body Irradiation</title><author>Baker, William H. ; Limanni, Alex ; Chang, Cheng-min ; Jackson, William E. ; Seemann, Ruth ; Patchen, Myra L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c334t-18f2f92d6c94e46c42364e4d1ce746a657c6d4a857d8e83e21d25b4b48b2551c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Biological effects of radiation</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. 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Results suggest that in vivo, ionizing radiation induces a time- and dose-dependent accumulation of IL-1α mRNA in the mouse spleen after exposure to γ radiation. Time-dependent increases in the level of IL-1α protein were also observed, although the magnitude of increased protein expression did not complement the magnitude of the accumulation of the message. Selective concentration of cells producing IL-1α does not appear to account completely for the increase in splenic IL-1α mRNA observed in this in vivo system.</abstract><cop>Oak Brook, Il</cop><pub>Radiation Research Society</pub><pmid>7652171</pmid><doi>10.2307/3579220</doi><tpages>7</tpages></addata></record> |
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| identifier | ISSN: 0033-7587 |
| ispartof | Radiation research, 1995-09, Vol.143 (3), p.320-326 |
| issn | 0033-7587 1938-5404 |
| language | eng |
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| source | Jstor Complete Legacy; MEDLINE |
| subjects | Animals Base Sequence Biological and medical sciences Biological effects of radiation Female Fundamental and applied biological sciences. Psychology Gene expression Gene Expression - radiation effects Interleukin-1 - analysis Interleukin-1 - genetics Ionizing radiation Ionizing radiations Irradiation Lymphocytes Messenger RNA Mice Molecular Sequence Data Radiation dosage RNA RNA, Messenger - analysis Space life sciences Spleen Spleen - metabolism Spleen cells Tissues, organs and organisms biophysics Whole-Body Irradiation |
| title | Comparison of Interleukin-1α Gene Expression and Protein Levels in the Murine Spleen after Lethal and Sublethal Total-Body Irradiation |
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