Rapid in vitro modulation of [ 3H]hemicholinium-3 binding sites in rat striatal slices
The effects of depolarizing concentration of potassium chloride on the modulation of [ 3H]hemicholinium-3 binding sites and high affinity choline uptake were examined in vitro. When rat striatal slices were incubated on Krebs buffer for 20 min, [ 3H]hemicholinium-3 binding sites diminished to 60% of...
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| Veröffentlicht in: | European journal of pharmacology 1987-03, Vol.135 (1), p.35-40 |
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| container_title | European journal of pharmacology |
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| creator | Saltarelli, Mario D. Lowenstein, Pedro R. Coyle, Joseph T. |
| description | The effects of depolarizing concentration of potassium chloride on the modulation of [
3H]hemicholinium-3 binding sites and high affinity choline uptake were examined in vitro. When rat striatal slices were incubated on Krebs buffer for 20 min, [
3H]hemicholinium-3 binding sites diminished to 60% of binding neasured in fresh un-incubated tissue, and remained stable for 60 min. Upon addition of Krebs buffer containing 40 mM KCl, the number of binding sites increased during a 20 min period, and remained stable for 40 min. Chanees in [
3H]hemicholinium-3 binding sites closely paralleled changes in high affinity choline uptake. Scatchrad analysis revealed that changes in binding result from alterations in the number of binding sites (B
max), and not in the affinity (K
D). These results suggest that neuronal depolarization rapidly alters the velocity of choline transport into cholinergic neurons by increasing the number of vailable carriers. |
| doi_str_mv | 10.1016/0014-2999(87)90754-0 |
| format | Article |
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3H]hemicholinium-3 binding sites and high affinity choline uptake were examined in vitro. When rat striatal slices were incubated on Krebs buffer for 20 min, [
3H]hemicholinium-3 binding sites diminished to 60% of binding neasured in fresh un-incubated tissue, and remained stable for 60 min. Upon addition of Krebs buffer containing 40 mM KCl, the number of binding sites increased during a 20 min period, and remained stable for 40 min. Chanees in [
3H]hemicholinium-3 binding sites closely paralleled changes in high affinity choline uptake. Scatchrad analysis revealed that changes in binding result from alterations in the number of binding sites (B
max), and not in the affinity (K
D). These results suggest that neuronal depolarization rapidly alters the velocity of choline transport into cholinergic neurons by increasing the number of vailable carriers.</description><identifier>ISSN: 0014-2999</identifier><identifier>EISSN: 1879-0712</identifier><identifier>DOI: 10.1016/0014-2999(87)90754-0</identifier><identifier>PMID: 3569424</identifier><identifier>CODEN: EJPHAZ</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>[ 3H]Hemicholinium-3 ; Animals ; Binding Sites ; Biological and medical sciences ; Choline - metabolism ; Cholinergic system ; Corpus Striatum - drug effects ; Corpus Striatum - metabolism ; Hemicholinium 3 - metabolism ; High affinity choline uptake ; In vitro regulation ; In Vitro Techniques ; Male ; Medical sciences ; Neuropharmacology ; Neurotransmitters. Neurotransmission. Receptors ; Pharmacology. Drug treatments ; Potassium Chloride - pharmacology ; Rats ; Rats, Inbred Strains ; Striatum</subject><ispartof>European journal of pharmacology, 1987-03, Vol.135 (1), p.35-40</ispartof><rights>1987</rights><rights>1988 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c367t-c94121e0dd47bf8616affb49428583e793b721d9454d72edd30d51e5ce1bfc843</citedby><cites>FETCH-LOGICAL-c367t-c94121e0dd47bf8616affb49428583e793b721d9454d72edd30d51e5ce1bfc843</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0014-2999(87)90754-0$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7474314$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3569424$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Saltarelli, Mario D.</creatorcontrib><creatorcontrib>Lowenstein, Pedro R.</creatorcontrib><creatorcontrib>Coyle, Joseph T.</creatorcontrib><title>Rapid in vitro modulation of [ 3H]hemicholinium-3 binding sites in rat striatal slices</title><title>European journal of pharmacology</title><addtitle>Eur J Pharmacol</addtitle><description>The effects of depolarizing concentration of potassium chloride on the modulation of [
3H]hemicholinium-3 binding sites and high affinity choline uptake were examined in vitro. When rat striatal slices were incubated on Krebs buffer for 20 min, [
3H]hemicholinium-3 binding sites diminished to 60% of binding neasured in fresh un-incubated tissue, and remained stable for 60 min. Upon addition of Krebs buffer containing 40 mM KCl, the number of binding sites increased during a 20 min period, and remained stable for 40 min. Chanees in [
3H]hemicholinium-3 binding sites closely paralleled changes in high affinity choline uptake. Scatchrad analysis revealed that changes in binding result from alterations in the number of binding sites (B
max), and not in the affinity (K
D). These results suggest that neuronal depolarization rapidly alters the velocity of choline transport into cholinergic neurons by increasing the number of vailable carriers.</description><subject>[ 3H]Hemicholinium-3</subject><subject>Animals</subject><subject>Binding Sites</subject><subject>Biological and medical sciences</subject><subject>Choline - metabolism</subject><subject>Cholinergic system</subject><subject>Corpus Striatum - drug effects</subject><subject>Corpus Striatum - metabolism</subject><subject>Hemicholinium 3 - metabolism</subject><subject>High affinity choline uptake</subject><subject>In vitro regulation</subject><subject>In Vitro Techniques</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Neuropharmacology</subject><subject>Neurotransmitters. Neurotransmission. Receptors</subject><subject>Pharmacology. Drug treatments</subject><subject>Potassium Chloride - pharmacology</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Striatum</subject><issn>0014-2999</issn><issn>1879-0712</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMFqFTEUhoMo7W31DRSyENHFaDJJJpNNQYpaoSCUtptSQiY5Y0-ZmdwmmYJv74z3cpeuzuJ8_38OHyFvOfvMGW--MMZlVRtjPrb6k2FayYq9IBvealMxzeuXZHNAjslJzo-MMWVqdUSOhGqMrOWG3F65LQaKE33GkiIdY5gHVzBONPb0joqL-wcY0T_EASecx0rQDqeA02-asUBek8kVmktCV9xA84Ae8mvyqndDhjf7eUpuvn-7Pr-oLn_9-Hn-9bLyotGl8kbymgMLQequbxveuL7v5PJaq1oB2ohO1zwYqWTQNYQgWFAclAfe9b6V4pR82PVuU3yaIRc7YvYwDG6COGertdRKSL6Acgf6FHNO0NttwtGlP5Yzu-q0qyu7urKttv90WrbE3u37526EcAjt_S379_u9y94NfXKTx3zAlutS8BU722GwuHhGSDZ7hMlDwAS-2BDx_3_8BVW8kG4</recordid><startdate>19870303</startdate><enddate>19870303</enddate><creator>Saltarelli, Mario D.</creator><creator>Lowenstein, Pedro R.</creator><creator>Coyle, Joseph T.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19870303</creationdate><title>Rapid in vitro modulation of [ 3H]hemicholinium-3 binding sites in rat striatal slices</title><author>Saltarelli, Mario D. ; Lowenstein, Pedro R. ; Coyle, Joseph T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c367t-c94121e0dd47bf8616affb49428583e793b721d9454d72edd30d51e5ce1bfc843</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>[ 3H]Hemicholinium-3</topic><topic>Animals</topic><topic>Binding Sites</topic><topic>Biological and medical sciences</topic><topic>Choline - metabolism</topic><topic>Cholinergic system</topic><topic>Corpus Striatum - drug effects</topic><topic>Corpus Striatum - metabolism</topic><topic>Hemicholinium 3 - metabolism</topic><topic>High affinity choline uptake</topic><topic>In vitro regulation</topic><topic>In Vitro Techniques</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Neuropharmacology</topic><topic>Neurotransmitters. Neurotransmission. Receptors</topic><topic>Pharmacology. Drug treatments</topic><topic>Potassium Chloride - pharmacology</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Striatum</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Saltarelli, Mario D.</creatorcontrib><creatorcontrib>Lowenstein, Pedro R.</creatorcontrib><creatorcontrib>Coyle, Joseph T.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Saltarelli, Mario D.</au><au>Lowenstein, Pedro R.</au><au>Coyle, Joseph T.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid in vitro modulation of [ 3H]hemicholinium-3 binding sites in rat striatal slices</atitle><jtitle>European journal of pharmacology</jtitle><addtitle>Eur J Pharmacol</addtitle><date>1987-03-03</date><risdate>1987</risdate><volume>135</volume><issue>1</issue><spage>35</spage><epage>40</epage><pages>35-40</pages><issn>0014-2999</issn><eissn>1879-0712</eissn><coden>EJPHAZ</coden><abstract>The effects of depolarizing concentration of potassium chloride on the modulation of [
3H]hemicholinium-3 binding sites and high affinity choline uptake were examined in vitro. When rat striatal slices were incubated on Krebs buffer for 20 min, [
3H]hemicholinium-3 binding sites diminished to 60% of binding neasured in fresh un-incubated tissue, and remained stable for 60 min. Upon addition of Krebs buffer containing 40 mM KCl, the number of binding sites increased during a 20 min period, and remained stable for 40 min. Chanees in [
3H]hemicholinium-3 binding sites closely paralleled changes in high affinity choline uptake. Scatchrad analysis revealed that changes in binding result from alterations in the number of binding sites (B
max), and not in the affinity (K
D). These results suggest that neuronal depolarization rapidly alters the velocity of choline transport into cholinergic neurons by increasing the number of vailable carriers.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>3569424</pmid><doi>10.1016/0014-2999(87)90754-0</doi><tpages>6</tpages></addata></record> |
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| identifier | ISSN: 0014-2999 |
| ispartof | European journal of pharmacology, 1987-03, Vol.135 (1), p.35-40 |
| issn | 0014-2999 1879-0712 |
| language | eng |
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| subjects | [ 3H]Hemicholinium-3 Animals Binding Sites Biological and medical sciences Choline - metabolism Cholinergic system Corpus Striatum - drug effects Corpus Striatum - metabolism Hemicholinium 3 - metabolism High affinity choline uptake In vitro regulation In Vitro Techniques Male Medical sciences Neuropharmacology Neurotransmitters. Neurotransmission. Receptors Pharmacology. Drug treatments Potassium Chloride - pharmacology Rats Rats, Inbred Strains Striatum |
| title | Rapid in vitro modulation of [ 3H]hemicholinium-3 binding sites in rat striatal slices |
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