Accessory cell function in a Con A response: Role of Ia and interleukin 1
Accessory cell (A-cell) function in a Con A response was analyzed. Irradiated P388D 1 cells efficiently induced a proliferative response to Con A of T cells purified from spleen cells, whereas paraformaldehyde-fixed P388D 1 cells failed to serve as A cells. Although IL-1 containing culture supernata...
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| Veröffentlicht in: | Cellular immunology 1987-05, Vol.106 (2), p.250-259 |
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| creator | Kohno, Keizo Kakiuchi, Terutaka Takeuchi, Makoto Taira, Sumiko Nariuchi, Hideo |
| description | Accessory cell (A-cell) function in a Con A response was analyzed. Irradiated P388D
1 cells efficiently induced a proliferative response to Con A of T cells purified from spleen cells, whereas paraformaldehyde-fixed P388D
1 cells failed to serve as A cells. Although IL-1 containing culture supernatant (SN) of a macrophage hybridoma induced the Con A response of the T-cell preparations, the depletion of Ia
+ cells by the treatment with anti-Ia antibody and complement abrogated the response in the presence of IL-1. Fixed P388D
1 cells and the hybridoma SN synergized in the reconstitution of the response. A 15,000-Da fraction of the hybridoma SN or human recombinant IL-1α was able to substitute the hybridoma SN for the response. The reconstitution of the response by IL-1 and fixed P388D
1 cells was inhibited by the addition of monoclonal anti-Ia antibody. These results indicate that IL-1 or fixed P388D
1 cell does not exert a sufficient signal by itself and both of them are required for the reconstitution of a Con A response of highly purified T cells, and that Ia on fixed P388D
1 cells play an important role. |
| doi_str_mv | 10.1016/0008-8749(87)90169-9 |
| format | Article |
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1 cells efficiently induced a proliferative response to Con A of T cells purified from spleen cells, whereas paraformaldehyde-fixed P388D
1 cells failed to serve as A cells. Although IL-1 containing culture supernatant (SN) of a macrophage hybridoma induced the Con A response of the T-cell preparations, the depletion of Ia
+ cells by the treatment with anti-Ia antibody and complement abrogated the response in the presence of IL-1. Fixed P388D
1 cells and the hybridoma SN synergized in the reconstitution of the response. A 15,000-Da fraction of the hybridoma SN or human recombinant IL-1α was able to substitute the hybridoma SN for the response. The reconstitution of the response by IL-1 and fixed P388D
1 cells was inhibited by the addition of monoclonal anti-Ia antibody. These results indicate that IL-1 or fixed P388D
1 cell does not exert a sufficient signal by itself and both of them are required for the reconstitution of a Con A response of highly purified T cells, and that Ia on fixed P388D
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1 cells efficiently induced a proliferative response to Con A of T cells purified from spleen cells, whereas paraformaldehyde-fixed P388D
1 cells failed to serve as A cells. Although IL-1 containing culture supernatant (SN) of a macrophage hybridoma induced the Con A response of the T-cell preparations, the depletion of Ia
+ cells by the treatment with anti-Ia antibody and complement abrogated the response in the presence of IL-1. Fixed P388D
1 cells and the hybridoma SN synergized in the reconstitution of the response. A 15,000-Da fraction of the hybridoma SN or human recombinant IL-1α was able to substitute the hybridoma SN for the response. The reconstitution of the response by IL-1 and fixed P388D
1 cells was inhibited by the addition of monoclonal anti-Ia antibody. These results indicate that IL-1 or fixed P388D
1 cell does not exert a sufficient signal by itself and both of them are required for the reconstitution of a Con A response of highly purified T cells, and that Ia on fixed P388D
1 cells play an important role.</description><subject>Animals</subject><subject>Antibodies, Monoclonal</subject><subject>Antigen-Presenting Cells - immunology</subject><subject>Antigens, Ly - immunology</subject><subject>Biological and medical sciences</subject><subject>Cell Line</subject><subject>Concanavalin A - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Histocompatibility Antigens Class II - immunology</subject><subject>Immunobiology</subject><subject>Immunological reactions in vitro</subject><subject>Interleukin-1 - immunology</subject><subject>Lymphoblastic transformation (stimulation by antigen, mitogen, mixed lymphocyte reactions)</subject><subject>Macrophages - immunology</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Spleen - cytology</subject><subject>T-Lymphocytes - classification</subject><subject>T-Lymphocytes - immunology</subject><issn>0008-8749</issn><issn>1090-2163</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1r3DAQhkVpSTZp_0EDOpSSHJyMZK0-cigsS5IuBAKhPQtZHoNar7WV7ED-feXussfmMho0z7wMDyGfGVwzYPIGAHSllTCXWl2Z8mMq844sGBioOJP1e7I4IqfkLOdfAIwJAyfkpBZGLAUsyGblPeYc0yv12Pe0mwY_hjjQMFBH16VZ0YR5F4eMt_Q59khjRzeOuqEtzIipx-l3gdlH8qFzfcZPh_ec_Ly_-7H-Xj0-PWzWq8fK1zUfK80Y99o1RoKquYCWyaVTjjdeKpBSK3DN0gvJuFNKGw8a_VIaYxoptNG8Pidf97m7FP9MmEe7DXm-3Q0Yp2yVEnNw_SbIhOaCG1NAsQd9ijkn7Owuha1Lr5aBnVXb2aOdPZZi_6m289rFIX9qttgelw5uy_zLYe6yd32X3OBDPmJKSFELXbBvewyLtJeAyWYfcPDYhoR-tG0M_7_jL3MBlmU</recordid><startdate>198705</startdate><enddate>198705</enddate><creator>Kohno, Keizo</creator><creator>Kakiuchi, Terutaka</creator><creator>Takeuchi, Makoto</creator><creator>Taira, Sumiko</creator><creator>Nariuchi, Hideo</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>198705</creationdate><title>Accessory cell function in a Con A response: Role of Ia and interleukin 1</title><author>Kohno, Keizo ; Kakiuchi, Terutaka ; Takeuchi, Makoto ; Taira, Sumiko ; Nariuchi, Hideo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c332t-8112c8ab96073240d165a7a2bc67066870ab5c4612a7789c08ec56999b6489823</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal</topic><topic>Antigen-Presenting Cells - immunology</topic><topic>Antigens, Ly - immunology</topic><topic>Biological and medical sciences</topic><topic>Cell Line</topic><topic>Concanavalin A - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Histocompatibility Antigens Class II - immunology</topic><topic>Immunobiology</topic><topic>Immunological reactions in vitro</topic><topic>Interleukin-1 - immunology</topic><topic>Lymphoblastic transformation (stimulation by antigen, mitogen, mixed lymphocyte reactions)</topic><topic>Macrophages - immunology</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Spleen - cytology</topic><topic>T-Lymphocytes - classification</topic><topic>T-Lymphocytes - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kohno, Keizo</creatorcontrib><creatorcontrib>Kakiuchi, Terutaka</creatorcontrib><creatorcontrib>Takeuchi, Makoto</creatorcontrib><creatorcontrib>Taira, Sumiko</creatorcontrib><creatorcontrib>Nariuchi, Hideo</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cellular immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kohno, Keizo</au><au>Kakiuchi, Terutaka</au><au>Takeuchi, Makoto</au><au>Taira, Sumiko</au><au>Nariuchi, Hideo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Accessory cell function in a Con A response: Role of Ia and interleukin 1</atitle><jtitle>Cellular immunology</jtitle><addtitle>Cell Immunol</addtitle><date>1987-05</date><risdate>1987</risdate><volume>106</volume><issue>2</issue><spage>250</spage><epage>259</epage><pages>250-259</pages><issn>0008-8749</issn><eissn>1090-2163</eissn><coden>CLIMB8</coden><abstract>Accessory cell (A-cell) function in a Con A response was analyzed. Irradiated P388D
1 cells efficiently induced a proliferative response to Con A of T cells purified from spleen cells, whereas paraformaldehyde-fixed P388D
1 cells failed to serve as A cells. Although IL-1 containing culture supernatant (SN) of a macrophage hybridoma induced the Con A response of the T-cell preparations, the depletion of Ia
+ cells by the treatment with anti-Ia antibody and complement abrogated the response in the presence of IL-1. Fixed P388D
1 cells and the hybridoma SN synergized in the reconstitution of the response. A 15,000-Da fraction of the hybridoma SN or human recombinant IL-1α was able to substitute the hybridoma SN for the response. The reconstitution of the response by IL-1 and fixed P388D
1 cells was inhibited by the addition of monoclonal anti-Ia antibody. These results indicate that IL-1 or fixed P388D
1 cell does not exert a sufficient signal by itself and both of them are required for the reconstitution of a Con A response of highly purified T cells, and that Ia on fixed P388D
1 cells play an important role.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>3494540</pmid><doi>10.1016/0008-8749(87)90169-9</doi><tpages>10</tpages></addata></record> |
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| ispartof | Cellular immunology, 1987-05, Vol.106 (2), p.250-259 |
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| subjects | Animals Antibodies, Monoclonal Antigen-Presenting Cells - immunology Antigens, Ly - immunology Biological and medical sciences Cell Line Concanavalin A - pharmacology Fundamental and applied biological sciences. Psychology Fundamental immunology Histocompatibility Antigens Class II - immunology Immunobiology Immunological reactions in vitro Interleukin-1 - immunology Lymphoblastic transformation (stimulation by antigen, mitogen, mixed lymphocyte reactions) Macrophages - immunology Mice Mice, Inbred BALB C Spleen - cytology T-Lymphocytes - classification T-Lymphocytes - immunology |
| title | Accessory cell function in a Con A response: Role of Ia and interleukin 1 |
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