[38] Biosynthesis of glycosylphosphatidylinositol anchors

This chapter describes radiolabeling techniques for studying glycosylphosphatidylinositol (GPI) biosynthesis by cell lysates, subcellular fractions, and permeabilized cells. GPIs are synthesized by all eukaryotic cells and are typically found covalently linked to cell surface glycoproteins. GPIs ser...

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Veröffentlicht in:	Methods in Enzymology 1995, Vol.250, p.513-535
Hauptverfasser:	Vidugiriene, Jolanta, Menon, Anant K.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bacterial Proteins Carbohydrate Conformation Carbohydrate Sequence Cell Line Cell Membrane - metabolism Cell Membrane Permeability Glycosylphosphatidylinositols - biosynthesis Guanosine Diphosphate Mannose - metabolism Indicators and Reagents Intracellular Membranes - metabolism Mammals Membrane Proteins - metabolism Mice Microsomes - metabolism Molecular Sequence Data Protein Processing, Post-Translational Radioisotope Dilution Technique Streptolysins Thymoma Thymus Neoplasms Toxoplasma - metabolism Tritium Trypanosoma - metabolism Tumor Cells, Cultured Uridine Diphosphate N-Acetylglucosamine - metabolism
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