Isolation of a biotin receptor from hepatic plasma membranes

Biotin, one of the growth promoting members of the 8 complex vitamin family, was found in the present investigation to have a specific receptor on isolated plasma membranes. Biotin bound to its receptor in a linear manner at 20°C with some binding as early as 30 minutes and full equilibrium binding...

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Veröffentlicht in:	Biochemical and biophysical research communications 1987-03, Vol.143 (3), p.913-916
Hauptverfasser:	Vesely, David L., Kemp, Stephen F., Elders, M.Joycelyn
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Applied sciences Biotin - metabolism Cell Membrane - metabolism Exact sciences and technology In Vitro Techniques Kinetics Liver - metabolism Mice Other techniques and industries Receptors, Cell Surface - isolation & purification Receptors, Cell Surface - metabolism Receptors, Growth Factor
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creator	Vesely, David L. Kemp, Stephen F. Elders, M.Joycelyn
description	Biotin, one of the growth promoting members of the 8 complex vitamin family, was found in the present investigation to have a specific receptor on isolated plasma membranes. Biotin bound to its receptor in a linear manner at 20°C with some binding as early as 30 minutes and full equilibrium binding being present at 20 hours. The best binding was found at 0.6 mg/ml of protein, but significant binding was still present at 0.6 μg/ml. The saturation of ligand binding sites was at 10 −7M. Half maximal saturation of binding was between 10 −9 and 10 −10M. These results demonstrate that a receptor for biotin does exist on purified plasma membranes.
doi_str_mv	10.1016/0006-291X(87)90336-6
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source	Elsevier ScienceDirect Journals Complete - AutoHoldings; MEDLINE
subjects	Animals Applied sciences Biotin - metabolism Cell Membrane - metabolism Exact sciences and technology In Vitro Techniques Kinetics Liver - metabolism Mice Other techniques and industries Receptors, Cell Surface - isolation & purification Receptors, Cell Surface - metabolism Receptors, Growth Factor
title	Isolation of a biotin receptor from hepatic plasma membranes
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