Transport and storage of Helicobacter pylori from gastric mucosal biopsies and clinical isolates
Various transport and storage conditions for the recovery of Helicobacter pylori from gastric biopsies were evaluated. Gastric mucosal biopsies from 16 Helicobacter pylori-infected patients were stored in cysteine-Albimi medium containing 20% glycerol in a refrigerator (4 degrees C) for 1 and 2 week...
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| Veröffentlicht in: | European journal of clinical microbiology & infectious diseases 1995-04, Vol.14 (4), p.349-352 |
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| container_title | European journal of clinical microbiology & infectious diseases |
| container_volume | 14 |
| creator | HAN, S. W FLAMM, R HACHEM, C. Y KIM, H. Y CLARRIDGE, J. E EVANS, D. G BEYER, J DRNEC, J GRAHAM, D. Y |
| description | Various transport and storage conditions for the recovery of Helicobacter pylori from gastric biopsies were evaluated. Gastric mucosal biopsies from 16 Helicobacter pylori-infected patients were stored in cysteine-Albimi medium containing 20% glycerol in a refrigerator (4 degrees C) for 1 and 2 weeks and in a -20 degrees C laboratory freezer for 4 and 12 weeks. Two clinical isolates were stored in saline, Stuart's transport media, cysteine-Albimi broth with 20% glycerol, brucella broth with 20% glycerol and skim milk with 17% glycerol at room temperature, 4 degrees C, -20 degrees C and -70 degrees C. Storage at 4 degrees C for 1 and 2 weeks resulted in Helicobacter pylori recovery from 81% and 19% of biopsies, respectively. Storage at -20 degrees C yielded Helicobacter pylori recovery in 100% and 57% after 4 and 12 weeks, respectively. At room temperature after 6 h, the Helicobacter pylori titer was reduced. The best storage media for frozen isolates were skim milk/glycerol, brucella broth/glycerol and cysteine-Albimi/glycerol (in descending order). Recovery was better at -70 degrees C than -20 degrees C. |
| doi_str_mv | 10.1007/BF02116531 |
| format | Article |
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W ; FLAMM, R ; HACHEM, C. Y ; KIM, H. Y ; CLARRIDGE, J. E ; EVANS, D. G ; BEYER, J ; DRNEC, J ; GRAHAM, D. Y</creator><creatorcontrib>HAN, S. W ; FLAMM, R ; HACHEM, C. Y ; KIM, H. Y ; CLARRIDGE, J. E ; EVANS, D. G ; BEYER, J ; DRNEC, J ; GRAHAM, D. Y</creatorcontrib><description>Various transport and storage conditions for the recovery of Helicobacter pylori from gastric biopsies were evaluated. Gastric mucosal biopsies from 16 Helicobacter pylori-infected patients were stored in cysteine-Albimi medium containing 20% glycerol in a refrigerator (4 degrees C) for 1 and 2 weeks and in a -20 degrees C laboratory freezer for 4 and 12 weeks. Two clinical isolates were stored in saline, Stuart's transport media, cysteine-Albimi broth with 20% glycerol, brucella broth with 20% glycerol and skim milk with 17% glycerol at room temperature, 4 degrees C, -20 degrees C and -70 degrees C. Storage at 4 degrees C for 1 and 2 weeks resulted in Helicobacter pylori recovery from 81% and 19% of biopsies, respectively. Storage at -20 degrees C yielded Helicobacter pylori recovery in 100% and 57% after 4 and 12 weeks, respectively. At room temperature after 6 h, the Helicobacter pylori titer was reduced. The best storage media for frozen isolates were skim milk/glycerol, brucella broth/glycerol and cysteine-Albimi/glycerol (in descending order). Recovery was better at -70 degrees C than -20 degrees C.</description><identifier>ISSN: 0934-9723</identifier><identifier>EISSN: 1435-4373</identifier><identifier>DOI: 10.1007/BF02116531</identifier><identifier>PMID: 7649201</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Bacterial diseases ; Bacterial diseases of the digestive system and abdomen ; Biological and medical sciences ; Biopsy ; Colony Count, Microbial ; Culture Media ; Gastric Mucosa - microbiology ; Gastric Mucosa - pathology ; Helicobacter Infections - pathology ; Helicobacter pylori ; Helicobacter pylori - growth & development ; Helicobacter pylori - isolation & purification ; Human bacterial diseases ; Humans ; Infectious diseases ; Male ; Medical sciences ; Peptic Ulcer - microbiology ; Peptic Ulcer - pathology ; Specimen Handling</subject><ispartof>European journal of clinical microbiology & infectious diseases, 1995-04, Vol.14 (4), p.349-352</ispartof><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c307t-8f18dc9cea85aaa5f8a97f18c32580c95c0b65f84f4e329debffe89b4c0aa89c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3497075$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7649201$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>HAN, S. 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Two clinical isolates were stored in saline, Stuart's transport media, cysteine-Albimi broth with 20% glycerol, brucella broth with 20% glycerol and skim milk with 17% glycerol at room temperature, 4 degrees C, -20 degrees C and -70 degrees C. Storage at 4 degrees C for 1 and 2 weeks resulted in Helicobacter pylori recovery from 81% and 19% of biopsies, respectively. Storage at -20 degrees C yielded Helicobacter pylori recovery in 100% and 57% after 4 and 12 weeks, respectively. At room temperature after 6 h, the Helicobacter pylori titer was reduced. The best storage media for frozen isolates were skim milk/glycerol, brucella broth/glycerol and cysteine-Albimi/glycerol (in descending order). Recovery was better at -70 degrees C than -20 degrees C.</description><subject>Bacterial diseases</subject><subject>Bacterial diseases of the digestive system and abdomen</subject><subject>Biological and medical sciences</subject><subject>Biopsy</subject><subject>Colony Count, Microbial</subject><subject>Culture Media</subject><subject>Gastric Mucosa - microbiology</subject><subject>Gastric Mucosa - pathology</subject><subject>Helicobacter Infections - pathology</subject><subject>Helicobacter pylori</subject><subject>Helicobacter pylori - growth & development</subject><subject>Helicobacter pylori - isolation & purification</subject><subject>Human bacterial diseases</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Peptic Ulcer - microbiology</subject><subject>Peptic Ulcer - pathology</subject><subject>Specimen Handling</subject><issn>0934-9723</issn><issn>1435-4373</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkDFLxDAYhoMo53m6uAsZxK2aNEmTjHp4nnDgcs71a5ockbapSTvcv7docXX64HkfXvhehK4puaeEyIenDckpLQSjJ2hJORMZZ5KdoiXRjGda5uwcXaT0SSZZSblAC1lwnRO6RB_7CF3qQxwwdDVOQ4hwsDg4vLWNN6ECM9iI-2MToscuhhYfIA3RG9yOJiRocOVDn7xNPwWm8Z03E_UpNDDYdInOHDTJXs13hd43z_v1Ntu9vbyuH3eZYUQOmXJU1UYbC0oAgHAKtJyYYblQxGhhSFVMlDtuWa5rWzlnla64IQBKG7ZCd7-9fQxfo01D2fpkbNNAZ8OYSil5QRVn_4pUCJXnuZzEm1kcq9bWZR99C_FYzttN-e2cQ5o-dtOQxqc_jXEtiRTsG51Tfk4</recordid><startdate>19950401</startdate><enddate>19950401</enddate><creator>HAN, S. 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Two clinical isolates were stored in saline, Stuart's transport media, cysteine-Albimi broth with 20% glycerol, brucella broth with 20% glycerol and skim milk with 17% glycerol at room temperature, 4 degrees C, -20 degrees C and -70 degrees C. Storage at 4 degrees C for 1 and 2 weeks resulted in Helicobacter pylori recovery from 81% and 19% of biopsies, respectively. Storage at -20 degrees C yielded Helicobacter pylori recovery in 100% and 57% after 4 and 12 weeks, respectively. At room temperature after 6 h, the Helicobacter pylori titer was reduced. The best storage media for frozen isolates were skim milk/glycerol, brucella broth/glycerol and cysteine-Albimi/glycerol (in descending order). Recovery was better at -70 degrees C than -20 degrees C.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>7649201</pmid><doi>10.1007/BF02116531</doi><tpages>4</tpages></addata></record> |
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| ispartof | European journal of clinical microbiology & infectious diseases, 1995-04, Vol.14 (4), p.349-352 |
| issn | 0934-9723 1435-4373 |
| language | eng |
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| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Bacterial diseases Bacterial diseases of the digestive system and abdomen Biological and medical sciences Biopsy Colony Count, Microbial Culture Media Gastric Mucosa - microbiology Gastric Mucosa - pathology Helicobacter Infections - pathology Helicobacter pylori Helicobacter pylori - growth & development Helicobacter pylori - isolation & purification Human bacterial diseases Humans Infectious diseases Male Medical sciences Peptic Ulcer - microbiology Peptic Ulcer - pathology Specimen Handling |
| title | Transport and storage of Helicobacter pylori from gastric mucosal biopsies and clinical isolates |
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