Effect of Vanadate on Amino Acid Transport in Rat Jejunum
Abstract Vanadate has been reported to inhibit (Na+ + K+)-ATPase of many cells and in some systems to stimulate adenylate cyclase. Since intestinal transport is influenced by these enzymes, we studied the effects of varying concentrations of orthovanadate (VO− 4) on alanine transport in the in vitro...
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| Veröffentlicht in: | Experimental biology and medicine (Maywood, N.J.) N.J.), 1987-04, Vol.184 (4), p.403-409 |
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| creator | Hajjar, Jean-Jacques Fucci, John C. Rowe, William A. Tomicic, Tatjana K. |
| description | Abstract
Vanadate has been reported to inhibit (Na+ + K+)-ATPase of many cells and in some systems to stimulate adenylate cyclase. Since intestinal transport is influenced by these enzymes, we studied the effects of varying concentrations of orthovanadate (VO−
4) on alanine transport in the in vitro rat jejunum. At the higher concentrations tested (10−3 and 10−2
M) vanadate had a ouabainlike action on alanine transport. It decreased the mucosal-to-serosal flux and the influx of alanine into the intestinal epithelium and it caused a reduction of (Na+ + K+)-ATPase activity of basolateral membranes. The relatively lower vanadate concentration of 10−4
M increased the influx and the efflux of alanine across the mucosal border of the jejunum. The increase was associated with elevation of cyclic AMP in the intestinal mucosa. The studies suggest the presence of a dual action of vanadate on amino acid transport, a stimulatory effect at low concentration, due to increased adenylate cyclase activity, and an inhibitory effect at higher concentrations, due to a decreased activity of (Na+ + K+)-ATPase. |
| doi_str_mv | 10.3181/00379727-184-42493 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_osti_</sourceid><recordid>TN_cdi_proquest_miscellaneous_77461664</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sage_id>10.3181_00379727-184-42493</sage_id><sourcerecordid>77461664</sourcerecordid><originalsourceid>FETCH-LOGICAL-c425t-1248a35bb2a8132bb8e40c5b21533e951e5d5e31d47b5cf4af34753cc4fe5d083</originalsourceid><addsrcrecordid>eNqFkMtKxDAUhoMoOl5eQBCCiLtqTi5NsxwGrwiCqNuQpol2mCZj0y58ezPO6FJXWfzf-XPOh9AxkAsGFVwSwqSSVBZQ8YJTrtgWmoBgomClUttosgKKFbGH9lOaEwJC0nIX7TLCoKzUBKkr750dcPT41QTTmMHhGPC0a0PEU9s2-Lk3IS1jP-A24Ccz4Hs3H8PYHaIdbxbJHW3eA_RyffU8uy0eHm_uZtOHwnIqhgIorwwTdU1NBYzWdeU4saKmeU_mlAAnGuEYNFzWwnpuPONSMGu5zwmp2AE6XffGNLQ62XZw9t3GEPLaugSRb1QZOl9Dyz5-jC4NumuTdYuFCS6OSUvJSyhL_i-Y-0pQfNVI16DtY0q983rZt53pPzUQvbKvf-zrbF9_289DJ5v2se5c8zuy0Z3zs01ukjULn9XaNv1ikiuoGGTsco0l8-b0PI59yIb_-vgLmfWWzg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15061949</pqid></control><display><type>article</type><title>Effect of Vanadate on Amino Acid Transport in Rat Jejunum</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>Hajjar, Jean-Jacques ; Fucci, John C. ; Rowe, William A. ; Tomicic, Tatjana K.</creator><creatorcontrib>Hajjar, Jean-Jacques ; Fucci, John C. ; Rowe, William A. ; Tomicic, Tatjana K. ; Veterans Administration Medical Center, Newington, CT</creatorcontrib><description>Abstract
Vanadate has been reported to inhibit (Na+ + K+)-ATPase of many cells and in some systems to stimulate adenylate cyclase. Since intestinal transport is influenced by these enzymes, we studied the effects of varying concentrations of orthovanadate (VO−
4) on alanine transport in the in vitro rat jejunum. At the higher concentrations tested (10−3 and 10−2
M) vanadate had a ouabainlike action on alanine transport. It decreased the mucosal-to-serosal flux and the influx of alanine into the intestinal epithelium and it caused a reduction of (Na+ + K+)-ATPase activity of basolateral membranes. The relatively lower vanadate concentration of 10−4
M increased the influx and the efflux of alanine across the mucosal border of the jejunum. The increase was associated with elevation of cyclic AMP in the intestinal mucosa. The studies suggest the presence of a dual action of vanadate on amino acid transport, a stimulatory effect at low concentration, due to increased adenylate cyclase activity, and an inhibitory effect at higher concentrations, due to a decreased activity of (Na+ + K+)-ATPase.</description><identifier>ISSN: 0037-9727</identifier><identifier>ISSN: 1535-3702</identifier><identifier>EISSN: 1535-3699</identifier><identifier>EISSN: 1525-1373</identifier><identifier>DOI: 10.3181/00379727-184-42493</identifier><identifier>PMID: 3031689</identifier><identifier>CODEN: PSEBAA</identifier><language>eng</language><publisher>London, England: SAGE Publications</publisher><subject>560300 - Chemicals Metabolism & Toxicology ; ABSORPTION ; ACID ANHYDRASES ; Adenosine Triphosphatases - metabolism ; Alanine - metabolism ; ALANINES ; AMINO ACIDS ; AMP ; ANIMALS ; ATP-ASE ; Biological and medical sciences ; Biological Transport - drug effects ; BODY ; Ca(2+) Mg(2+)-ATPase - metabolism ; CARBOXYLIC ACIDS ; Cyclic AMP - metabolism ; DIGESTIVE SYSTEM ; DOSE-RESPONSE RELATIONSHIPS ; ELEMENTS ; ENZYMES ; Fundamental and applied biological sciences. Psychology ; GASTROINTESTINAL TRACT ; HYDROLASES ; In Vitro Techniques ; INHIBITION ; INTESTINAL ABSORPTION ; Intestinal Mucosa - metabolism ; Intestine. Mesentery ; INTESTINES ; jejunum ; Jejunum - metabolism ; Kinetics ; Male ; MAMMALS ; METALS ; Microvilli - drug effects ; Microvilli - metabolism ; NUCLEOTIDES ; ORGANIC ACIDS ; ORGANIC COMPOUNDS ; ORGANS ; PHOSPHOHYDROLASES ; RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT ; RATS ; Rats, Inbred Strains ; RODENTS ; SMALL INTESTINE ; Sodium-Potassium-Exchanging ATPase - metabolism ; STIMULATION ; TOXICITY ; TRANSITION ELEMENTS ; UPTAKE ; vanadate ; Vanadates ; VANADIUM ; Vanadium - pharmacology ; VERTEBRATES ; Vertebrates: digestive system</subject><ispartof>Experimental biology and medicine (Maywood, N.J.), 1987-04, Vol.184 (4), p.403-409</ispartof><rights>1988 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c425t-1248a35bb2a8132bb8e40c5b21533e951e5d5e31d47b5cf4af34753cc4fe5d083</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,881,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7491831$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3031689$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/6150039$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Hajjar, Jean-Jacques</creatorcontrib><creatorcontrib>Fucci, John C.</creatorcontrib><creatorcontrib>Rowe, William A.</creatorcontrib><creatorcontrib>Tomicic, Tatjana K.</creatorcontrib><creatorcontrib>Veterans Administration Medical Center, Newington, CT</creatorcontrib><title>Effect of Vanadate on Amino Acid Transport in Rat Jejunum</title><title>Experimental biology and medicine (Maywood, N.J.)</title><addtitle>Proc Soc Exp Biol Med</addtitle><description>Abstract
Vanadate has been reported to inhibit (Na+ + K+)-ATPase of many cells and in some systems to stimulate adenylate cyclase. Since intestinal transport is influenced by these enzymes, we studied the effects of varying concentrations of orthovanadate (VO−
4) on alanine transport in the in vitro rat jejunum. At the higher concentrations tested (10−3 and 10−2
M) vanadate had a ouabainlike action on alanine transport. It decreased the mucosal-to-serosal flux and the influx of alanine into the intestinal epithelium and it caused a reduction of (Na+ + K+)-ATPase activity of basolateral membranes. The relatively lower vanadate concentration of 10−4
M increased the influx and the efflux of alanine across the mucosal border of the jejunum. The increase was associated with elevation of cyclic AMP in the intestinal mucosa. The studies suggest the presence of a dual action of vanadate on amino acid transport, a stimulatory effect at low concentration, due to increased adenylate cyclase activity, and an inhibitory effect at higher concentrations, due to a decreased activity of (Na+ + K+)-ATPase.</description><subject>560300 - Chemicals Metabolism & Toxicology</subject><subject>ABSORPTION</subject><subject>ACID ANHYDRASES</subject><subject>Adenosine Triphosphatases - metabolism</subject><subject>Alanine - metabolism</subject><subject>ALANINES</subject><subject>AMINO ACIDS</subject><subject>AMP</subject><subject>ANIMALS</subject><subject>ATP-ASE</subject><subject>Biological and medical sciences</subject><subject>Biological Transport - drug effects</subject><subject>BODY</subject><subject>Ca(2+) Mg(2+)-ATPase - metabolism</subject><subject>CARBOXYLIC ACIDS</subject><subject>Cyclic AMP - metabolism</subject><subject>DIGESTIVE SYSTEM</subject><subject>DOSE-RESPONSE RELATIONSHIPS</subject><subject>ELEMENTS</subject><subject>ENZYMES</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GASTROINTESTINAL TRACT</subject><subject>HYDROLASES</subject><subject>In Vitro Techniques</subject><subject>INHIBITION</subject><subject>INTESTINAL ABSORPTION</subject><subject>Intestinal Mucosa - metabolism</subject><subject>Intestine. Mesentery</subject><subject>INTESTINES</subject><subject>jejunum</subject><subject>Jejunum - metabolism</subject><subject>Kinetics</subject><subject>Male</subject><subject>MAMMALS</subject><subject>METALS</subject><subject>Microvilli - drug effects</subject><subject>Microvilli - metabolism</subject><subject>NUCLEOTIDES</subject><subject>ORGANIC ACIDS</subject><subject>ORGANIC COMPOUNDS</subject><subject>ORGANS</subject><subject>PHOSPHOHYDROLASES</subject><subject>RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT</subject><subject>RATS</subject><subject>Rats, Inbred Strains</subject><subject>RODENTS</subject><subject>SMALL INTESTINE</subject><subject>Sodium-Potassium-Exchanging ATPase - metabolism</subject><subject>STIMULATION</subject><subject>TOXICITY</subject><subject>TRANSITION ELEMENTS</subject><subject>UPTAKE</subject><subject>vanadate</subject><subject>Vanadates</subject><subject>VANADIUM</subject><subject>Vanadium - pharmacology</subject><subject>VERTEBRATES</subject><subject>Vertebrates: digestive system</subject><issn>0037-9727</issn><issn>1535-3702</issn><issn>1535-3699</issn><issn>1525-1373</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtKxDAUhoMoOl5eQBCCiLtqTi5NsxwGrwiCqNuQpol2mCZj0y58ezPO6FJXWfzf-XPOh9AxkAsGFVwSwqSSVBZQ8YJTrtgWmoBgomClUttosgKKFbGH9lOaEwJC0nIX7TLCoKzUBKkr750dcPT41QTTmMHhGPC0a0PEU9s2-Lk3IS1jP-A24Ccz4Hs3H8PYHaIdbxbJHW3eA_RyffU8uy0eHm_uZtOHwnIqhgIorwwTdU1NBYzWdeU4saKmeU_mlAAnGuEYNFzWwnpuPONSMGu5zwmp2AE6XffGNLQ62XZw9t3GEPLaugSRb1QZOl9Dyz5-jC4NumuTdYuFCS6OSUvJSyhL_i-Y-0pQfNVI16DtY0q983rZt53pPzUQvbKvf-zrbF9_289DJ5v2se5c8zuy0Z3zs01ukjULn9XaNv1ikiuoGGTsco0l8-b0PI59yIb_-vgLmfWWzg</recordid><startdate>19870401</startdate><enddate>19870401</enddate><creator>Hajjar, Jean-Jacques</creator><creator>Fucci, John C.</creator><creator>Rowe, William A.</creator><creator>Tomicic, Tatjana K.</creator><general>SAGE Publications</general><general>Blackwell Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>19870401</creationdate><title>Effect of Vanadate on Amino Acid Transport in Rat Jejunum</title><author>Hajjar, Jean-Jacques ; Fucci, John C. ; Rowe, William A. ; Tomicic, Tatjana K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c425t-1248a35bb2a8132bb8e40c5b21533e951e5d5e31d47b5cf4af34753cc4fe5d083</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>560300 - Chemicals Metabolism & Toxicology</topic><topic>ABSORPTION</topic><topic>ACID ANHYDRASES</topic><topic>Adenosine Triphosphatases - metabolism</topic><topic>Alanine - metabolism</topic><topic>ALANINES</topic><topic>AMINO ACIDS</topic><topic>AMP</topic><topic>ANIMALS</topic><topic>ATP-ASE</topic><topic>Biological and medical sciences</topic><topic>Biological Transport - drug effects</topic><topic>BODY</topic><topic>Ca(2+) Mg(2+)-ATPase - metabolism</topic><topic>CARBOXYLIC ACIDS</topic><topic>Cyclic AMP - metabolism</topic><topic>DIGESTIVE SYSTEM</topic><topic>DOSE-RESPONSE RELATIONSHIPS</topic><topic>ELEMENTS</topic><topic>ENZYMES</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GASTROINTESTINAL TRACT</topic><topic>HYDROLASES</topic><topic>In Vitro Techniques</topic><topic>INHIBITION</topic><topic>INTESTINAL ABSORPTION</topic><topic>Intestinal Mucosa - metabolism</topic><topic>Intestine. Mesentery</topic><topic>INTESTINES</topic><topic>jejunum</topic><topic>Jejunum - metabolism</topic><topic>Kinetics</topic><topic>Male</topic><topic>MAMMALS</topic><topic>METALS</topic><topic>Microvilli - drug effects</topic><topic>Microvilli - metabolism</topic><topic>NUCLEOTIDES</topic><topic>ORGANIC ACIDS</topic><topic>ORGANIC COMPOUNDS</topic><topic>ORGANS</topic><topic>PHOSPHOHYDROLASES</topic><topic>RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT</topic><topic>RATS</topic><topic>Rats, Inbred Strains</topic><topic>RODENTS</topic><topic>SMALL INTESTINE</topic><topic>Sodium-Potassium-Exchanging ATPase - metabolism</topic><topic>STIMULATION</topic><topic>TOXICITY</topic><topic>TRANSITION ELEMENTS</topic><topic>UPTAKE</topic><topic>vanadate</topic><topic>Vanadates</topic><topic>VANADIUM</topic><topic>Vanadium - pharmacology</topic><topic>VERTEBRATES</topic><topic>Vertebrates: digestive system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hajjar, Jean-Jacques</creatorcontrib><creatorcontrib>Fucci, John C.</creatorcontrib><creatorcontrib>Rowe, William A.</creatorcontrib><creatorcontrib>Tomicic, Tatjana K.</creatorcontrib><creatorcontrib>Veterans Administration Medical Center, Newington, CT</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Experimental biology and medicine (Maywood, N.J.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hajjar, Jean-Jacques</au><au>Fucci, John C.</au><au>Rowe, William A.</au><au>Tomicic, Tatjana K.</au><aucorp>Veterans Administration Medical Center, Newington, CT</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of Vanadate on Amino Acid Transport in Rat Jejunum</atitle><jtitle>Experimental biology and medicine (Maywood, N.J.)</jtitle><addtitle>Proc Soc Exp Biol Med</addtitle><date>1987-04-01</date><risdate>1987</risdate><volume>184</volume><issue>4</issue><spage>403</spage><epage>409</epage><pages>403-409</pages><issn>0037-9727</issn><issn>1535-3702</issn><eissn>1535-3699</eissn><eissn>1525-1373</eissn><coden>PSEBAA</coden><abstract>Abstract
Vanadate has been reported to inhibit (Na+ + K+)-ATPase of many cells and in some systems to stimulate adenylate cyclase. Since intestinal transport is influenced by these enzymes, we studied the effects of varying concentrations of orthovanadate (VO−
4) on alanine transport in the in vitro rat jejunum. At the higher concentrations tested (10−3 and 10−2
M) vanadate had a ouabainlike action on alanine transport. It decreased the mucosal-to-serosal flux and the influx of alanine into the intestinal epithelium and it caused a reduction of (Na+ + K+)-ATPase activity of basolateral membranes. The relatively lower vanadate concentration of 10−4
M increased the influx and the efflux of alanine across the mucosal border of the jejunum. The increase was associated with elevation of cyclic AMP in the intestinal mucosa. The studies suggest the presence of a dual action of vanadate on amino acid transport, a stimulatory effect at low concentration, due to increased adenylate cyclase activity, and an inhibitory effect at higher concentrations, due to a decreased activity of (Na+ + K+)-ATPase.</abstract><cop>London, England</cop><pub>SAGE Publications</pub><pmid>3031689</pmid><doi>10.3181/00379727-184-42493</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0037-9727 |
| ispartof | Experimental biology and medicine (Maywood, N.J.), 1987-04, Vol.184 (4), p.403-409 |
| issn | 0037-9727 1535-3702 1535-3699 1525-1373 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_77461664 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | 560300 - Chemicals Metabolism & Toxicology ABSORPTION ACID ANHYDRASES Adenosine Triphosphatases - metabolism Alanine - metabolism ALANINES AMINO ACIDS AMP ANIMALS ATP-ASE Biological and medical sciences Biological Transport - drug effects BODY Ca(2+) Mg(2+)-ATPase - metabolism CARBOXYLIC ACIDS Cyclic AMP - metabolism DIGESTIVE SYSTEM DOSE-RESPONSE RELATIONSHIPS ELEMENTS ENZYMES Fundamental and applied biological sciences. Psychology GASTROINTESTINAL TRACT HYDROLASES In Vitro Techniques INHIBITION INTESTINAL ABSORPTION Intestinal Mucosa - metabolism Intestine. Mesentery INTESTINES jejunum Jejunum - metabolism Kinetics Male MAMMALS METALS Microvilli - drug effects Microvilli - metabolism NUCLEOTIDES ORGANIC ACIDS ORGANIC COMPOUNDS ORGANS PHOSPHOHYDROLASES RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT RATS Rats, Inbred Strains RODENTS SMALL INTESTINE Sodium-Potassium-Exchanging ATPase - metabolism STIMULATION TOXICITY TRANSITION ELEMENTS UPTAKE vanadate Vanadates VANADIUM Vanadium - pharmacology VERTEBRATES Vertebrates: digestive system |
| title | Effect of Vanadate on Amino Acid Transport in Rat Jejunum |
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