Real-time calcium response of cultured bone cells to fluid flow

Using a parallel-plate flow chamber and fura-2 fluorescence microscopy, intracellular calcium was measured cell by cell in preconfluent primary culture rat calvarial bone cells to 18, 35, and 70 dynes/cm2 of fluid-induced shear stress. A heterogeneous response with respect to peak amplitude and late...

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Veröffentlicht in:	Clinical orthopaedics and related research 1995-04, Vol.313 (313), p.256-269
Hauptverfasser:	HUNG, C. T, POLLACK, S. R, REILLY, T. M, BRIGHTON, C. T
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Bone and Bones - cytology Bone and Bones - metabolism Calcium - metabolism Cells, Cultured Fundamental and applied biological sciences. Psychology Inositol Phosphates - physiology Microscopy, Fluorescence Osteoblasts - metabolism Osteoblasts - physiology Rats Rats, Sprague-Dawley Rheology Skeleton and joints Space life sciences Stress, Mechanical Time Factors Vertebrates: osteoarticular system, musculoskeletal system
Online-Zugang:	Volltext
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