Acetylcarnitine-mediated inhibition of ethanol oxidation in hepatocytes
Carnitine-mediated prevention of ethanol-induced hepatic steatosis is related to the attenuation of ethanol metabolism by carnitine in the intact rat. Although carnitine retards ethanol oxidation in the intact animal, the in vitro activities of ethainol-metabolizing enzymes remain unaltered. Therefo...
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| Veröffentlicht in: | Alcohol (Fayetteville, N.Y.) N.Y.), 1995-05, Vol.12 (3), p.289-294 |
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| container_title | Alcohol (Fayetteville, N.Y.) |
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| creator | Cha, Youn-Soo Sachan, Dileep S |
| description | Carnitine-mediated prevention of ethanol-induced hepatic steatosis is related to the attenuation of ethanol metabolism by carnitine in the intact rat. Although carnitine retards ethanol oxidation in the intact animal, the in vitro activities of ethainol-metabolizing enzymes remain unaltered. Therefore, hepatocytes were targeted to understand the mechanism of carnitine effect on ethanol metabolism. Rat hepatocytes were isolated by a collagenase-perfusion technique and incubated in albumin-containing medium with ethanol in the presence or absence of added carnitine or related compounds. Ethanol oxidation was determined by the loss of ethanol as well as by the products formed. The rate of ethanol oxidation in the presence of carnitine was one-half the rate in the absence of carnitine (14 vs. 25 nmol · min
−1 · million
−1 cells). It took 100 times the concentration of carnitine to equal the maximal inhibition produced by acetylcarnitine and the effect of acetylcanitine was without a lag time. It is concluded that acetylcarnitine is the mediator of carnitine inhibition of ethanol oxidation. |
| doi_str_mv | 10.1016/0741-8329(95)00007-E |
| format | Article |
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−1 · million
−1 cells). It took 100 times the concentration of carnitine to equal the maximal inhibition produced by acetylcarnitine and the effect of acetylcanitine was without a lag time. It is concluded that acetylcarnitine is the mediator of carnitine inhibition of ethanol oxidation.</description><identifier>ISSN: 0741-8329</identifier><identifier>EISSN: 1873-6823</identifier><identifier>DOI: 10.1016/0741-8329(95)00007-E</identifier><identifier>PMID: 7639964</identifier><identifier>CODEN: ALCOEX</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Acetylcarnitine ; Acetylcarnitine - pharmacology ; Alcoholism and acute alcohol poisoning ; Animals ; Biological and medical sciences ; Carnitine ; Carnitine - pharmacology ; Cell Separation ; Choline ; Ethanol - metabolism ; Hepatocytes ; Liver - cytology ; Liver - metabolism ; Lysine ; Male ; Medical sciences ; Oxidation-Reduction - drug effects ; Rat ; Rats ; Rats, Sprague-Dawley ; Toxicology ; γ-Butyrobetaine</subject><ispartof>Alcohol (Fayetteville, N.Y.), 1995-05, Vol.12 (3), p.289-294</ispartof><rights>1995</rights><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-1a85ac0b8aa4a4d338fc4b77e9588377f33d89dc68efee3911ca1437d09aaa563</citedby><cites>FETCH-LOGICAL-c417t-1a85ac0b8aa4a4d338fc4b77e9588377f33d89dc68efee3911ca1437d09aaa563</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0741-8329(95)00007-E$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3573819$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7639964$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cha, Youn-Soo</creatorcontrib><creatorcontrib>Sachan, Dileep S</creatorcontrib><title>Acetylcarnitine-mediated inhibition of ethanol oxidation in hepatocytes</title><title>Alcohol (Fayetteville, N.Y.)</title><addtitle>Alcohol</addtitle><description>Carnitine-mediated prevention of ethanol-induced hepatic steatosis is related to the attenuation of ethanol metabolism by carnitine in the intact rat. Although carnitine retards ethanol oxidation in the intact animal, the in vitro activities of ethainol-metabolizing enzymes remain unaltered. Therefore, hepatocytes were targeted to understand the mechanism of carnitine effect on ethanol metabolism. Rat hepatocytes were isolated by a collagenase-perfusion technique and incubated in albumin-containing medium with ethanol in the presence or absence of added carnitine or related compounds. Ethanol oxidation was determined by the loss of ethanol as well as by the products formed. The rate of ethanol oxidation in the presence of carnitine was one-half the rate in the absence of carnitine (14 vs. 25 nmol · min
−1 · million
−1 cells). It took 100 times the concentration of carnitine to equal the maximal inhibition produced by acetylcarnitine and the effect of acetylcanitine was without a lag time. It is concluded that acetylcarnitine is the mediator of carnitine inhibition of ethanol oxidation.</description><subject>Acetylcarnitine</subject><subject>Acetylcarnitine - pharmacology</subject><subject>Alcoholism and acute alcohol poisoning</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Carnitine</subject><subject>Carnitine - pharmacology</subject><subject>Cell Separation</subject><subject>Choline</subject><subject>Ethanol - metabolism</subject><subject>Hepatocytes</subject><subject>Liver - cytology</subject><subject>Liver - metabolism</subject><subject>Lysine</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Oxidation-Reduction - drug effects</subject><subject>Rat</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Toxicology</subject><subject>γ-Butyrobetaine</subject><issn>0741-8329</issn><issn>1873-6823</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtLAzEQgIMoWh__QGEPInpY3ZjsJrkIUuoDBC96DtPsLI1sk5qkYv-9WVt61DAQmPlmGL4h5JRW17SizU0lOC0lu1WXqr6q8hPlZIeMqBSsbOQt2yWjLXJADmP8GBgh1D7ZFw1TquEj8nhvMK16A8HZZB2Wc2wtJGwL62Z2mnPeFb4rMM3A-b7w37aF36R1xQwXkLxZJYzHZK-DPuLJ5j8i7w-Tt_FT-fL6-Dy-fykNpyKVFGQNpppKAA68ZUx2hk-FQFVLyYToGGulak0jsUNkilIDlDPRVgoA6oYdkYv13EXwn0uMSc9tNNj34NAvoxaCc8Fy_AfSRlWKyQHka9AEH2PATi-CnUNYaVrpQbQeLOrBola1_hWtJ7ntbDN_Oc3Otk0bs7l-vqlDNNB3AZyxcYuxWjBJVcbu1hhmaV8Wg47GojP5CgFN0q23f-_xA6DVmjw</recordid><startdate>19950501</startdate><enddate>19950501</enddate><creator>Cha, Youn-Soo</creator><creator>Sachan, Dileep S</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>19950501</creationdate><title>Acetylcarnitine-mediated inhibition of ethanol oxidation in hepatocytes</title><author>Cha, Youn-Soo ; Sachan, Dileep S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-1a85ac0b8aa4a4d338fc4b77e9588377f33d89dc68efee3911ca1437d09aaa563</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Acetylcarnitine</topic><topic>Acetylcarnitine - pharmacology</topic><topic>Alcoholism and acute alcohol poisoning</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Carnitine</topic><topic>Carnitine - pharmacology</topic><topic>Cell Separation</topic><topic>Choline</topic><topic>Ethanol - metabolism</topic><topic>Hepatocytes</topic><topic>Liver - cytology</topic><topic>Liver - metabolism</topic><topic>Lysine</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Oxidation-Reduction - drug effects</topic><topic>Rat</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Toxicology</topic><topic>γ-Butyrobetaine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cha, Youn-Soo</creatorcontrib><creatorcontrib>Sachan, Dileep S</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Alcohol (Fayetteville, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cha, Youn-Soo</au><au>Sachan, Dileep S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Acetylcarnitine-mediated inhibition of ethanol oxidation in hepatocytes</atitle><jtitle>Alcohol (Fayetteville, N.Y.)</jtitle><addtitle>Alcohol</addtitle><date>1995-05-01</date><risdate>1995</risdate><volume>12</volume><issue>3</issue><spage>289</spage><epage>294</epage><pages>289-294</pages><issn>0741-8329</issn><eissn>1873-6823</eissn><coden>ALCOEX</coden><abstract>Carnitine-mediated prevention of ethanol-induced hepatic steatosis is related to the attenuation of ethanol metabolism by carnitine in the intact rat. Although carnitine retards ethanol oxidation in the intact animal, the in vitro activities of ethainol-metabolizing enzymes remain unaltered. Therefore, hepatocytes were targeted to understand the mechanism of carnitine effect on ethanol metabolism. Rat hepatocytes were isolated by a collagenase-perfusion technique and incubated in albumin-containing medium with ethanol in the presence or absence of added carnitine or related compounds. Ethanol oxidation was determined by the loss of ethanol as well as by the products formed. The rate of ethanol oxidation in the presence of carnitine was one-half the rate in the absence of carnitine (14 vs. 25 nmol · min
−1 · million
−1 cells). It took 100 times the concentration of carnitine to equal the maximal inhibition produced by acetylcarnitine and the effect of acetylcanitine was without a lag time. It is concluded that acetylcarnitine is the mediator of carnitine inhibition of ethanol oxidation.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>7639964</pmid><doi>10.1016/0741-8329(95)00007-E</doi><tpages>6</tpages></addata></record> |
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| ispartof | Alcohol (Fayetteville, N.Y.), 1995-05, Vol.12 (3), p.289-294 |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Acetylcarnitine Acetylcarnitine - pharmacology Alcoholism and acute alcohol poisoning Animals Biological and medical sciences Carnitine Carnitine - pharmacology Cell Separation Choline Ethanol - metabolism Hepatocytes Liver - cytology Liver - metabolism Lysine Male Medical sciences Oxidation-Reduction - drug effects Rat Rats Rats, Sprague-Dawley Toxicology γ-Butyrobetaine |
| title | Acetylcarnitine-mediated inhibition of ethanol oxidation in hepatocytes |
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