Improved hepatocyte in vitro maintenance in a culture model with woven multicompartment capillary systems: Electron microscopy studies
Primary pig hepatocytes form a tissuelike structure in an in vitro culture model that has provision for three-dimensional cell orientation, cell aggregation, decentralized cell perfusion with low metabolite gradients, integral oxygenation, and nonparenchymal cell coculture. Scanning electron microsc...
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| Veröffentlicht in: | Hepatology (Baltimore, Md.) Md.), 1995-08, Vol.22 (2), p.546-552 |
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| container_title | Hepatology (Baltimore, Md.) |
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| creator | Gerlach, Jörg C. Schnoy, Norbert Encke, Jens Smith, Mark D. Müller, Christian Neuhaus, Peter |
| description | Primary pig hepatocytes form a tissuelike structure in an
in vitro culture model that has provision for three-dimensional cell orientation, cell aggregation, decentralized cell perfusion with low metabolite gradients, integral oxygenation, and nonparenchymal cell coculture. Scanning electron microscopy (SEM) has shown that hepatocytes spontaneously form aggregates in a three-dimensional structure between and on the surface of artificial capillaries. Transmission electron microscopy (TEM) has shown that after 7 weeks of
in vitro perfusion, the cell ultrastructure remains similar to that of the parenchyma
in vivo. Golgi complexes, active membrane processes, reorganization of cell junctions, and bile canaliculi-like intercellular spaces were demonstrated. |
| doi_str_mv | 10.1016/0270-9139(95)90578-2 |
| format | Article |
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in vitro perfusion, the cell ultrastructure remains similar to that of the parenchyma
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in vitro perfusion, the cell ultrastructure remains similar to that of the parenchyma
in vivo. Golgi complexes, active membrane processes, reorganization of cell junctions, and bile canaliculi-like intercellular spaces were demonstrated.</description><subject>Animals</subject><subject>Bile Canaliculi - ultrastructure</subject><subject>Biological and medical sciences</subject><subject>Capillaries</subject><subject>Cell Aggregation</subject><subject>Cell Membrane - ultrastructure</subject><subject>Cells, Cultured</subject><subject>Cytological Techniques</subject><subject>Development. Metamorphosis. Moult. Ageing</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Golgi Apparatus - ultrastructure</subject><subject>Intercellular Junctions - ultrastructure</subject><subject>Liver - physiology</subject><subject>Liver - ultrastructure</subject><subject>Male</subject><subject>Microscopy, Electron</subject><subject>Microscopy, Electron, Scanning</subject><subject>Mitochondria, Liver - ultrastructure</subject><subject>Perfusion</subject><subject>Swine</subject><subject>Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><issn>0270-9139</issn><issn>1527-3350</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc1u1DAUhS1EVaYDbwCSFwjBIuDEcRx3gYSq_kmV2MDacuwb1ShOgu1MNS_Q5-amM5olK1s-51yd-5mQ9yX7WrKy-cYqyQpVcvVZiS-KCdkW1SuyKUUlC84Fe002J8sbcpHSH8aYqqv2nJzLhou64hvyfB_mOO3A0UeYTZ7sPgP1I935HCcajB8zjGa0L4-G2mXISwQaJgcDffL5kT5heqQBBW-nMJuYA4yZWjP7YTBxT9M-ZQjpkl4PYHEqmr2NU7LTjGJenIf0lpz1Zkjw7nhuye-b619Xd8XDz9v7qx8PheVtk4tWqEYIYzveda6TphalEUxYJpS1jYMWrwDMoavsWwlKMA4N66FuneoxtiWfDnNx6b8LpKyDTxaw6AjTkrSUdS05ktmS-mBcm6YIvZ6jD7iOLple8euVrV7ZaiX0C369xj4c5y9dAHcKHXmj_vGom2TN0EdE69PJxpuayVai7fvBBshi5yHqZD3gLzgfkaF2k_9_j39q4qVJ</recordid><startdate>19950801</startdate><enddate>19950801</enddate><creator>Gerlach, Jörg C.</creator><creator>Schnoy, Norbert</creator><creator>Encke, Jens</creator><creator>Smith, Mark D.</creator><creator>Müller, Christian</creator><creator>Neuhaus, Peter</creator><general>Elsevier Inc</general><general>Wiley</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19950801</creationdate><title>Improved hepatocyte in vitro maintenance in a culture model with woven multicompartment capillary systems: Electron microscopy studies</title><author>Gerlach, Jörg C. ; Schnoy, Norbert ; Encke, Jens ; Smith, Mark D. ; Müller, Christian ; Neuhaus, Peter</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-859655acb3bbdb7a451a505c059cc6de85c0ee0d9651f87e9503e60fe48d9fcb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Animals</topic><topic>Bile Canaliculi - ultrastructure</topic><topic>Biological and medical sciences</topic><topic>Capillaries</topic><topic>Cell Aggregation</topic><topic>Cell Membrane - ultrastructure</topic><topic>Cells, Cultured</topic><topic>Cytological Techniques</topic><topic>Development. Metamorphosis. Moult. Ageing</topic><topic>Fundamental and applied biological sciences. 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| ispartof | Hepatology (Baltimore, Md.), 1995-08, Vol.22 (2), p.546-552 |
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| language | eng |
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| subjects | Animals Bile Canaliculi - ultrastructure Biological and medical sciences Capillaries Cell Aggregation Cell Membrane - ultrastructure Cells, Cultured Cytological Techniques Development. Metamorphosis. Moult. Ageing Fundamental and applied biological sciences. Psychology Golgi Apparatus - ultrastructure Intercellular Junctions - ultrastructure Liver - physiology Liver - ultrastructure Male Microscopy, Electron Microscopy, Electron, Scanning Mitochondria, Liver - ultrastructure Perfusion Swine Vertebrates: anatomy and physiology, studies on body, several organs or systems |
| title | Improved hepatocyte in vitro maintenance in a culture model with woven multicompartment capillary systems: Electron microscopy studies |
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