Biotin synthase from Escherichia coli, an investigation of the low molecular weight and protein components required for activity in vitro

We have developed a radiochemical method for the measurement of biotin synthase activity in vitro. A cell-free extract from an Escherichia coli strain containing a cloned bioB (biotin synthase) gene was incubated with [14C]dethiobiotin, which was converted to [14C] biotin. The assay was used to iden...

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Veröffentlicht in:	The Journal of biological chemistry 1995-08, Vol.270 (32), p.19158-19165
Hauptverfasser:	Birch, O M, Fuhrmann, M, Shaw, N M
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Cysteine - metabolism Escherichia coli Escherichia coli - enzymology Molecular Sequence Data Molecular Weight Oxidoreductases - isolation & purification Sulfurtransferases - chemistry Sulfurtransferases - metabolism
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