Differential bioassay of interleukin 2 and interleukin 4
The T cell-derived lymphokines interleukin 2 (IL-2) and interleukin 4 (IL-4, originally BSF-1) both exhibit T cell growth-promoting activity. Recent observations that T cell lines commonly used as indicator cells in IL-2 bioassays also proliferate in response to IL-4 demonstrate the lack of specific...
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| Veröffentlicht in: | Journal of immunological methods 1987-04, Vol.98 (1), p.99-104 |
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| container_title | Journal of immunological methods |
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| creator | Ho, Steffan N. Abraham, Robert T. Gillis, Steven McKean, David J. |
| description | The T cell-derived lymphokines interleukin 2 (IL-2) and interleukin 4 (IL-4, originally BSF-1) both exhibit T cell growth-promoting activity. Recent observations that T cell lines commonly used as indicator cells in IL-2 bioassays also proliferate in response to IL-4 demonstrate the lack of specificity of these bioassays for IL-2. In this report we describe a bioassay method to differentiate IL-2 and IL-4 through the parallel use of two T cell lines with defined lymphokine specificity. The IL-2-responsive CT6 cell line, when used in conjunction with the IL-2- and IL-4-responsive HT-2 cell line, allows for the differentiation of IL-2 and IL-4 in conditioned media. |
| doi_str_mv | 10.1016/0022-1759(87)90441-8 |
| format | Article |
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Recent observations that T cell lines commonly used as indicator cells in IL-2 bioassays also proliferate in response to IL-4 demonstrate the lack of specificity of these bioassays for IL-2. In this report we describe a bioassay method to differentiate IL-2 and IL-4 through the parallel use of two T cell lines with defined lymphokine specificity. The IL-2-responsive CT6 cell line, when used in conjunction with the IL-2- and IL-4-responsive HT-2 cell line, allows for the differentiation of IL-2 and IL-4 in conditioned media.</description><identifier>ISSN: 0022-1759</identifier><identifier>EISSN: 1872-7905</identifier><identifier>DOI: 10.1016/0022-1759(87)90441-8</identifier><identifier>PMID: 3494085</identifier><identifier>CODEN: JIMMBG</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Biological and medical sciences ; Biological Assay - methods ; Cell Line ; Fundamental and applied biological sciences. 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Recent observations that T cell lines commonly used as indicator cells in IL-2 bioassays also proliferate in response to IL-4 demonstrate the lack of specificity of these bioassays for IL-2. In this report we describe a bioassay method to differentiate IL-2 and IL-4 through the parallel use of two T cell lines with defined lymphokine specificity. The IL-2-responsive CT6 cell line, when used in conjunction with the IL-2- and IL-4-responsive HT-2 cell line, allows for the differentiation of IL-2 and IL-4 in conditioned media.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biological Assay - methods</subject><subject>Cell Line</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Growth Substances - analysis</subject><subject>Humans</subject><subject>Interleukin-2</subject><subject>Interleukin-2 - analysis</subject><subject>Interleukin-4</subject><subject>Lymphocyte Activation - drug effects</subject><subject>Lymphokine bioassay</subject><subject>Lymphokines - analysis</subject><subject>Molecular immunology</subject><subject>Rats</subject><subject>Recombinant Proteins - analysis</subject><subject>T-Lymphocytes - immunology</subject><subject>Techniques</subject><issn>0022-1759</issn><issn>1872-7905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LAzEQhoMotVb_gcIeRPSwOvnYTXIRpH5CwUvvIZudQHS7W5Ot0H_v1paCF08DM8_7MjyEnFO4pUDLOwDGcioLfa3kjQYhaK4OyJgqyXKpoTgk4z1yTE5S-gAACiWMyIgLLUAVY6Ieg_cYse2DbbIqdDYlu846n4W2x9jg6jO0GctsW__ZiFNy5G2T8Gw3J2T-_DSfvuaz95e36cMsd1yVfS4dL6UT4AtZi7K2nCEUuqiUUIxb7aXwFUVkVe0tUMoqi4wKyqnTrgLOJ-RqW7uM3dcKU28WITlsGttit0pGSiEKLWEAxRZ0sUspojfLGBY2rg0Fs_FlNjLMRoZR0vz6MmqIXez6V9UC631oJ2i4X-7uNjnb-GhbF9IeU5wzBnrA7rcYDiq-A0aTXMDWYR0iut7UXfj_jx-ucIUI</recordid><startdate>19870402</startdate><enddate>19870402</enddate><creator>Ho, Steffan N.</creator><creator>Abraham, Robert T.</creator><creator>Gillis, Steven</creator><creator>McKean, David J.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19870402</creationdate><title>Differential bioassay of interleukin 2 and interleukin 4</title><author>Ho, Steffan N. ; Abraham, Robert T. ; Gillis, Steven ; McKean, David J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-7c367c40f57d46da32e0595b84823a9f74fb1ee2bdfa0112bae214131c9cb033</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biological Assay - methods</topic><topic>Cell Line</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Growth Substances - analysis</topic><topic>Humans</topic><topic>Interleukin-2</topic><topic>Interleukin-2 - analysis</topic><topic>Interleukin-4</topic><topic>Lymphocyte Activation - drug effects</topic><topic>Lymphokine bioassay</topic><topic>Lymphokines - analysis</topic><topic>Molecular immunology</topic><topic>Rats</topic><topic>Recombinant Proteins - analysis</topic><topic>T-Lymphocytes - immunology</topic><topic>Techniques</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ho, Steffan N.</creatorcontrib><creatorcontrib>Abraham, Robert T.</creatorcontrib><creatorcontrib>Gillis, Steven</creatorcontrib><creatorcontrib>McKean, David J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of immunological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ho, Steffan N.</au><au>Abraham, Robert T.</au><au>Gillis, Steven</au><au>McKean, David J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differential bioassay of interleukin 2 and interleukin 4</atitle><jtitle>Journal of immunological methods</jtitle><addtitle>J Immunol Methods</addtitle><date>1987-04-02</date><risdate>1987</risdate><volume>98</volume><issue>1</issue><spage>99</spage><epage>104</epage><pages>99-104</pages><issn>0022-1759</issn><eissn>1872-7905</eissn><coden>JIMMBG</coden><abstract>The T cell-derived lymphokines interleukin 2 (IL-2) and interleukin 4 (IL-4, originally BSF-1) both exhibit T cell growth-promoting activity. Recent observations that T cell lines commonly used as indicator cells in IL-2 bioassays also proliferate in response to IL-4 demonstrate the lack of specificity of these bioassays for IL-2. In this report we describe a bioassay method to differentiate IL-2 and IL-4 through the parallel use of two T cell lines with defined lymphokine specificity. The IL-2-responsive CT6 cell line, when used in conjunction with the IL-2- and IL-4-responsive HT-2 cell line, allows for the differentiation of IL-2 and IL-4 in conditioned media.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>3494085</pmid><doi>10.1016/0022-1759(87)90441-8</doi><tpages>6</tpages></addata></record> |
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| ispartof | Journal of immunological methods, 1987-04, Vol.98 (1), p.99-104 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Biological and medical sciences Biological Assay - methods Cell Line Fundamental and applied biological sciences. Psychology Fundamental immunology Growth Substances - analysis Humans Interleukin-2 Interleukin-2 - analysis Interleukin-4 Lymphocyte Activation - drug effects Lymphokine bioassay Lymphokines - analysis Molecular immunology Rats Recombinant Proteins - analysis T-Lymphocytes - immunology Techniques |
| title | Differential bioassay of interleukin 2 and interleukin 4 |
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