The separation and identification of glutathione S-transferase subunits from Orthosia gothica

Four subunits of the cytosolic glutathione S-transferase (GST) in Orthosia gothica fed on willow leaves and a semisynthetic bean diet were purified as separate peaks (subunits 1–4) by a two-step gradient elution from a reverse-phase HPLC column after an initial purification by glutathione-Sepharose...

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Veröffentlicht in:	Insect biochemistry and molecular biology 1995-07, Vol.25 (7), p.783-788
Hauptverfasser:	Egaas, Eliann, Sandvik, Morten, Svendsen, Nina O., Skaare, Janneche U.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Chromatography, Affinity Chromatography, High Pressure Liquid Dietary components Dinitrochlorobenzene - metabolism Female Glutathione Transferase - analysis Glutathione Transferase - classification Glutathione-Sepharose affinity matrix Induction Insect glutathione Lepidoptera Lepidoptera Larvae Molecular Sequence Data Moths - enzymology N-terminal amino acid sequence Nitrobenzenes - metabolism Noctuidae Orthosia gothica Reverse-phase HPLC S-transferase Sequence Homology, Amino Acid Substrate Specificity Subunits
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