Isolation and Characterization of a Spontaneously Arising Long-Lived Line of Human Keratinocytes (NM 1)

The long-lived keratinocyte line, NM 1, was isolated from the epidermis of a pool of foreskins obtained from apparently normal neonates at the time of circumcision. Cultures were initiated in Dulbecco's minimal essential medium containing 20% fetal bovine serum,$0.4 \mug/ml$hydrocortisone, 10-9...

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