Evaluation of the single radial-immunodiffusion assay for measuring the glycoprotein content of rabies vaccines
The glycoprotein content of rabies vaccines containing the Pitman—Moore strain of rabies virus was measured by the single radial immunodiffusion assay and correlated with vaccine potency. The variability of this assay was 6·3% for a single vaccine lot tested over a one-year period. Using sera prepar...
Gespeichert in:
| Veröffentlicht in: | Journal of biological standardization 1987, Vol.15 (1), p.1-10 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 10 |
|---|---|
| container_issue | 1 |
| container_start_page | 1 |
| container_title | Journal of biological standardization |
| container_volume | 15 |
| creator | Mayner, Ronald E. Needy, Charles F. |
| description | The glycoprotein content of rabies vaccines containing the Pitman—Moore strain of rabies virus was measured by the single radial immunodiffusion assay and correlated with vaccine potency. The variability of this assay was 6·3% for a single vaccine lot tested over a one-year period. Using sera prepared against rabies virus glycoprotein from different strains of virus, the assay gave different values. These differences could be eliminated by using a homologous vaccine strain as an internal reference. Single radial-immunodiffusion values for Pitman—Moore vaccines correlated with the manufacturers' NIH potency assay, but required a mathematical transformation to convert values from one assay to the other. Single radial-immunodiffusion values for Street Alabama Dufferin and Flury-LEP vaccines did not correlate with NIH values. Modification of the single radial immunodiffusion technique and the feasibility of using this assay for the determination of rabies vaccine potency are discussed. |
| doi_str_mv | 10.1016/0092-1157(87)90011-4 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_77443130</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0092115787900114</els_id><sourcerecordid>77443130</sourcerecordid><originalsourceid>FETCH-LOGICAL-c417t-f6a999d8e4fc52973aa87705ed4ff4a504487872f190c499f4546f15d27babbd3</originalsourceid><addsrcrecordid>eNqFkUFv1DAQhX0AlbLlH4CUA0JwSLFjZ-1ckKqqFKRKvZSzNbHHxSixiydZaf89SXe1R3qy5Pne08x7jL0X_FJwsf3KedfUQrT6s9FfOs6FqNUrdn76fsPeEv3hvDGqNWfsTAqupOLnLN_sYJhhijlVOVTTb6wopscBqwI-wlDHcZxT9jGEmVYIiGBfhVyqEYHmssDPqsdh7_JTyRPGVLmcJkzT6ligj0jVDpyLCemCvQ4wEL47vhv26_vNw_WP-u7-9uf11V3tlNBTHbbQdZ03qIJrm05LAKM1b9GrEBS0XCmjjW6C6LhTXRdUq7ZBtL7RPfS9lxv26eC7rPR3RprsGMnhMEDCPJPVWikpJH8RFGqJTEqzgOoAupKJCgb7VOIIZW8Ft2sJdk3brmlbo-1zCVYtsg9H_7kf0Z9ExwaW-cfjHMjBEAokF-mEmUaK7XL_hn07YLiEtotYLLmIyaGPBd1kfY7_3-MfF_umXg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>14845338</pqid></control><display><type>article</type><title>Evaluation of the single radial-immunodiffusion assay for measuring the glycoprotein content of rabies vaccines</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>Mayner, Ronald E. ; Needy, Charles F.</creator><creatorcontrib>Mayner, Ronald E. ; Needy, Charles F.</creatorcontrib><description>The glycoprotein content of rabies vaccines containing the Pitman—Moore strain of rabies virus was measured by the single radial immunodiffusion assay and correlated with vaccine potency. The variability of this assay was 6·3% for a single vaccine lot tested over a one-year period. Using sera prepared against rabies virus glycoprotein from different strains of virus, the assay gave different values. These differences could be eliminated by using a homologous vaccine strain as an internal reference. Single radial-immunodiffusion values for Pitman—Moore vaccines correlated with the manufacturers' NIH potency assay, but required a mathematical transformation to convert values from one assay to the other. Single radial-immunodiffusion values for Street Alabama Dufferin and Flury-LEP vaccines did not correlate with NIH values. Modification of the single radial immunodiffusion technique and the feasibility of using this assay for the determination of rabies vaccine potency are discussed.</description><identifier>ISSN: 0092-1157</identifier><identifier>DOI: 10.1016/0092-1157(87)90011-4</identifier><identifier>PMID: 3104340</identifier><identifier>CODEN: JBSTBI</identifier><language>eng</language><publisher>New York, NY: Elsevier Science (USA)</publisher><subject>Antibody Specificity ; Biological and medical sciences ; Detergents ; Fundamental and applied biological sciences. Psychology ; Glycoproteins - immunology ; Immunodiffusion - methods ; Microbiology ; Polyethylene Glycols ; Rabies Vaccines - immunology ; rabies virus ; Rabies virus - immunology ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies ; Viral Proteins - immunology ; Virology</subject><ispartof>Journal of biological standardization, 1987, Vol.15 (1), p.1-10</ispartof><rights>1987</rights><rights>1987 INIST-CNRS</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-f6a999d8e4fc52973aa87705ed4ff4a504487872f190c499f4546f15d27babbd3</citedby><cites>FETCH-LOGICAL-c417t-f6a999d8e4fc52973aa87705ed4ff4a504487872f190c499f4546f15d27babbd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8231697$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3104340$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mayner, Ronald E.</creatorcontrib><creatorcontrib>Needy, Charles F.</creatorcontrib><title>Evaluation of the single radial-immunodiffusion assay for measuring the glycoprotein content of rabies vaccines</title><title>Journal of biological standardization</title><addtitle>J Biol Stand</addtitle><description>The glycoprotein content of rabies vaccines containing the Pitman—Moore strain of rabies virus was measured by the single radial immunodiffusion assay and correlated with vaccine potency. The variability of this assay was 6·3% for a single vaccine lot tested over a one-year period. Using sera prepared against rabies virus glycoprotein from different strains of virus, the assay gave different values. These differences could be eliminated by using a homologous vaccine strain as an internal reference. Single radial-immunodiffusion values for Pitman—Moore vaccines correlated with the manufacturers' NIH potency assay, but required a mathematical transformation to convert values from one assay to the other. Single radial-immunodiffusion values for Street Alabama Dufferin and Flury-LEP vaccines did not correlate with NIH values. Modification of the single radial immunodiffusion technique and the feasibility of using this assay for the determination of rabies vaccine potency are discussed.</description><subject>Antibody Specificity</subject><subject>Biological and medical sciences</subject><subject>Detergents</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glycoproteins - immunology</subject><subject>Immunodiffusion - methods</subject><subject>Microbiology</subject><subject>Polyethylene Glycols</subject><subject>Rabies Vaccines - immunology</subject><subject>rabies virus</subject><subject>Rabies virus - immunology</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</subject><subject>Viral Proteins - immunology</subject><subject>Virology</subject><issn>0092-1157</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFv1DAQhX0AlbLlH4CUA0JwSLFjZ-1ckKqqFKRKvZSzNbHHxSixiydZaf89SXe1R3qy5Pne08x7jL0X_FJwsf3KedfUQrT6s9FfOs6FqNUrdn76fsPeEv3hvDGqNWfsTAqupOLnLN_sYJhhijlVOVTTb6wopscBqwI-wlDHcZxT9jGEmVYIiGBfhVyqEYHmssDPqsdh7_JTyRPGVLmcJkzT6ligj0jVDpyLCemCvQ4wEL47vhv26_vNw_WP-u7-9uf11V3tlNBTHbbQdZ03qIJrm05LAKM1b9GrEBS0XCmjjW6C6LhTXRdUq7ZBtL7RPfS9lxv26eC7rPR3RprsGMnhMEDCPJPVWikpJH8RFGqJTEqzgOoAupKJCgb7VOIIZW8Ft2sJdk3brmlbo-1zCVYtsg9H_7kf0Z9ExwaW-cfjHMjBEAokF-mEmUaK7XL_hn07YLiEtotYLLmIyaGPBd1kfY7_3-MfF_umXg</recordid><startdate>1987</startdate><enddate>1987</enddate><creator>Mayner, Ronald E.</creator><creator>Needy, Charles F.</creator><general>Elsevier Science (USA)</general><general>Academic Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>1987</creationdate><title>Evaluation of the single radial-immunodiffusion assay for measuring the glycoprotein content of rabies vaccines</title><author>Mayner, Ronald E. ; Needy, Charles F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-f6a999d8e4fc52973aa87705ed4ff4a504487872f190c499f4546f15d27babbd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Antibody Specificity</topic><topic>Biological and medical sciences</topic><topic>Detergents</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glycoproteins - immunology</topic><topic>Immunodiffusion - methods</topic><topic>Microbiology</topic><topic>Polyethylene Glycols</topic><topic>Rabies Vaccines - immunology</topic><topic>rabies virus</topic><topic>Rabies virus - immunology</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</topic><topic>Viral Proteins - immunology</topic><topic>Virology</topic><toplevel>online_resources</toplevel><creatorcontrib>Mayner, Ronald E.</creatorcontrib><creatorcontrib>Needy, Charles F.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biological standardization</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mayner, Ronald E.</au><au>Needy, Charles F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of the single radial-immunodiffusion assay for measuring the glycoprotein content of rabies vaccines</atitle><jtitle>Journal of biological standardization</jtitle><addtitle>J Biol Stand</addtitle><date>1987</date><risdate>1987</risdate><volume>15</volume><issue>1</issue><spage>1</spage><epage>10</epage><pages>1-10</pages><issn>0092-1157</issn><coden>JBSTBI</coden><abstract>The glycoprotein content of rabies vaccines containing the Pitman—Moore strain of rabies virus was measured by the single radial immunodiffusion assay and correlated with vaccine potency. The variability of this assay was 6·3% for a single vaccine lot tested over a one-year period. Using sera prepared against rabies virus glycoprotein from different strains of virus, the assay gave different values. These differences could be eliminated by using a homologous vaccine strain as an internal reference. Single radial-immunodiffusion values for Pitman—Moore vaccines correlated with the manufacturers' NIH potency assay, but required a mathematical transformation to convert values from one assay to the other. Single radial-immunodiffusion values for Street Alabama Dufferin and Flury-LEP vaccines did not correlate with NIH values. Modification of the single radial immunodiffusion technique and the feasibility of using this assay for the determination of rabies vaccine potency are discussed.</abstract><cop>New York, NY</cop><pub>Elsevier Science (USA)</pub><pmid>3104340</pmid><doi>10.1016/0092-1157(87)90011-4</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0092-1157 |
| ispartof | Journal of biological standardization, 1987, Vol.15 (1), p.1-10 |
| issn | 0092-1157 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_77443130 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Antibody Specificity Biological and medical sciences Detergents Fundamental and applied biological sciences. Psychology Glycoproteins - immunology Immunodiffusion - methods Microbiology Polyethylene Glycols Rabies Vaccines - immunology rabies virus Rabies virus - immunology Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies Viral Proteins - immunology Virology |
| title | Evaluation of the single radial-immunodiffusion assay for measuring the glycoprotein content of rabies vaccines |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-21T14%3A18%3A17IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Evaluation%20of%20the%20single%20radial-immunodiffusion%20assay%20for%20measuring%20the%20glycoprotein%20content%20of%20rabies%20vaccines&rft.jtitle=Journal%20of%20biological%20standardization&rft.au=Mayner,%20Ronald%20E.&rft.date=1987&rft.volume=15&rft.issue=1&rft.spage=1&rft.epage=10&rft.pages=1-10&rft.issn=0092-1157&rft.coden=JBSTBI&rft_id=info:doi/10.1016/0092-1157(87)90011-4&rft_dat=%3Cproquest_cross%3E77443130%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=14845338&rft_id=info:pmid/3104340&rft_els_id=0092115787900114&rfr_iscdi=true |