Improved detection of nitric oxide radical (NO.) production in an activated macrophage culture with a radical scavenger, carboxy PTIO and Griess reagent
An improved method for the detection of nitric oxide radicals (NO. in cultures of activated macrophages was developed, involving a nitric oxide radical scavenger, 2-(4-carboxyphenyl)-4,4,5,5- tetramethylimidazoline-3-oxide-1-oxyl (carboxy PTIO) and Griess reagent. A murine macrophage-like cell line,...
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| Veröffentlicht in: | FEBS letters 1995-07, Vol.368 (3), p.425-428 |
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| container_title | FEBS letters |
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| creator | Amano, F Noda, T |
| description | An improved method for the detection of nitric oxide radicals (NO. in cultures of activated macrophages was developed, involving a nitric oxide radical scavenger, 2-(4-carboxyphenyl)-4,4,5,5- tetramethylimidazoline-3-oxide-1-oxyl (carboxy PTIO) and Griess reagent. A murine macrophage-like cell line, J774.1, was activated with interferon-gamma (IFN-gamma) and bacterial lipopolysaccharide (LPS), which induced the production and secretion of NO2- into the culture supernatant. Addition of carboxy PTIO to the activated macrophages increased the amount of NO2- to 4- to 5-fold without cell damages, probably because carboxy PTIO rapidly reacted with NO. to form NO2-, which was finally assayed by the Griess reaction. |
| doi_str_mv | 10.1016/0014-5793(95)00700-J |
| format | Article |
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| ispartof | FEBS letters, 1995-07, Vol.368 (3), p.425-428 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Amino Acid Oxidoreductases - chemistry Animals Benzoates Cell Line Cyclic N-Oxides - chemistry Dose-Response Relationship, Drug Ethylenediamines Free Radical Scavengers - chemistry Free Radicals Imidazoles - chemistry Indicators and Reagents Macrophage Activation Macrophages - chemistry Mice Nitric Oxide - biosynthesis Nitric Oxide - chemistry Nitric Oxide Synthase Sulfanilamides |
| title | Improved detection of nitric oxide radical (NO.) production in an activated macrophage culture with a radical scavenger, carboxy PTIO and Griess reagent |
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