Decoding of cytosolic calcium oscillations in the mitochondria
Frequency-modulated oscillations of cytosolic Ca 2+ ([Ca 2+] c) are believed to be important in signal transduction, but it has been difficult to correlate [Ca 2+] c oscillations directly with the activity of Ca 2+-regulated targets. We have studied the control of Ca 2+-sensitive mitochondrial dehyd...
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Veröffentlicht in: | Cell 1995-08, Vol.82 (3), p.415-424 |
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creator | Hajnóczky, György Robb-Gaspers, Lawrence D Seitz, Michele B Thomas, Andrew P |
description | Frequency-modulated oscillations of cytosolic Ca
2+ ([Ca
2+]
c) are believed to be important in signal transduction, but it has been difficult to correlate [Ca
2+]
c oscillations directly with the activity of Ca
2+-regulated targets. We have studied the control of Ca
2+-sensitive mitochondrial dehydrogenases (CSMDHs) by monitoring mitochondria) Ca
2+ ([Ca
2+]
m) and the redox state of flavoproteins and pyridine nucleotides simultaneously with [Ca
2+]
c in single hepatocytes. Oscillations of [Ca
2+]
c induced by IP
3-dependent hormones were efficiently transmitted to the mitochondria as [Ca
2+]
m oscillations. Each [Ca
2+]
m spike was sufficient to cause a maximal transient activation of the CSMDHs and [Ca
2+]
m oscillations at frequencies above 0.5 per minute caused a sustained activation of mitochondrial metabolism. By contrast, sustained [Ca
2+]
c increases yielded only transient CSMDH activation, and slow or partial [Ca
2+]
c elevations were ineffective in increasing [Ca
2+]
m or stimulating CSMDHs. We conclude that the mitochondria are tuned to oscillating [Ca
2+]
c signals, the frequency of which can control the CSMDHs over the full range of potential activities. |
doi_str_mv | 10.1016/0092-8674(95)90430-1 |
format | Article |
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2+ ([Ca
2+]
c) are believed to be important in signal transduction, but it has been difficult to correlate [Ca
2+]
c oscillations directly with the activity of Ca
2+-regulated targets. We have studied the control of Ca
2+-sensitive mitochondrial dehydrogenases (CSMDHs) by monitoring mitochondria) Ca
2+ ([Ca
2+]
m) and the redox state of flavoproteins and pyridine nucleotides simultaneously with [Ca
2+]
c in single hepatocytes. Oscillations of [Ca
2+]
c induced by IP
3-dependent hormones were efficiently transmitted to the mitochondria as [Ca
2+]
m oscillations. Each [Ca
2+]
m spike was sufficient to cause a maximal transient activation of the CSMDHs and [Ca
2+]
m oscillations at frequencies above 0.5 per minute caused a sustained activation of mitochondrial metabolism. By contrast, sustained [Ca
2+]
c increases yielded only transient CSMDH activation, and slow or partial [Ca
2+]
c elevations were ineffective in increasing [Ca
2+]
m or stimulating CSMDHs. We conclude that the mitochondria are tuned to oscillating [Ca
2+]
c signals, the frequency of which can control the CSMDHs over the full range of potential activities.</description><identifier>ISSN: 0092-8674</identifier><identifier>EISSN: 1097-4172</identifier><identifier>DOI: 10.1016/0092-8674(95)90430-1</identifier><identifier>PMID: 7634331</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Calcium - physiology ; Liver - physiology ; Male ; Mitochondria, Liver - physiology ; Oxidation-Reduction ; Oxidoreductases - physiology ; Rats ; Rats, Sprague-Dawley</subject><ispartof>Cell, 1995-08, Vol.82 (3), p.415-424</ispartof><rights>1995</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c469t-c5c3eaf5a52e3243b44f1b8fa49453b99e9d64a31982c5cb7813d8a90004d93a3</citedby><cites>FETCH-LOGICAL-c469t-c5c3eaf5a52e3243b44f1b8fa49453b99e9d64a31982c5cb7813d8a90004d93a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0092-8674(95)90430-1$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7634331$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hajnóczky, György</creatorcontrib><creatorcontrib>Robb-Gaspers, Lawrence D</creatorcontrib><creatorcontrib>Seitz, Michele B</creatorcontrib><creatorcontrib>Thomas, Andrew P</creatorcontrib><title>Decoding of cytosolic calcium oscillations in the mitochondria</title><title>Cell</title><addtitle>Cell</addtitle><description>Frequency-modulated oscillations of cytosolic Ca
2+ ([Ca
2+]
c) are believed to be important in signal transduction, but it has been difficult to correlate [Ca
2+]
c oscillations directly with the activity of Ca
2+-regulated targets. We have studied the control of Ca
2+-sensitive mitochondrial dehydrogenases (CSMDHs) by monitoring mitochondria) Ca
2+ ([Ca
2+]
m) and the redox state of flavoproteins and pyridine nucleotides simultaneously with [Ca
2+]
c in single hepatocytes. Oscillations of [Ca
2+]
c induced by IP
3-dependent hormones were efficiently transmitted to the mitochondria as [Ca
2+]
m oscillations. Each [Ca
2+]
m spike was sufficient to cause a maximal transient activation of the CSMDHs and [Ca
2+]
m oscillations at frequencies above 0.5 per minute caused a sustained activation of mitochondrial metabolism. By contrast, sustained [Ca
2+]
c increases yielded only transient CSMDH activation, and slow or partial [Ca
2+]
c elevations were ineffective in increasing [Ca
2+]
m or stimulating CSMDHs. We conclude that the mitochondria are tuned to oscillating [Ca
2+]
c signals, the frequency of which can control the CSMDHs over the full range of potential activities.</description><subject>Animals</subject><subject>Calcium - physiology</subject><subject>Liver - physiology</subject><subject>Male</subject><subject>Mitochondria, Liver - physiology</subject><subject>Oxidation-Reduction</subject><subject>Oxidoreductases - physiology</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><issn>0092-8674</issn><issn>1097-4172</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM1OwzAQhC0EKqXwBiDlhOAQsGM7ji9IqPxKlbjA2XLsDTVK4mInSH17XFr1yGkPMzs7-yF0TvANwaS8xVgWeVUKdiX5tcSM4pwcoCnBUuSMiOIQTfeWY3QS4xfGuOKcT9BElJRRSqbo7gGMt67_zHyTmfXgo2-dyYxujRu7zEfj2lYPzvcxc302LCHr3ODN0vc2OH2KjhrdRjjbzRn6eHp8n7_ki7fn1_n9IjeslENuuKGgG655AbRgtGasIXXVaCYZp7WUIG3JNCWyKpK3FhWhttIyFWZWUk1n6HKbuwr-e4Q4qM5FA6laD36MSghGywrTZGRbowk-xgCNWgXX6bBWBKsNNrVhojZMlOTqD5siae1ilz_WHdj90o5T0u-2OqQnfxwElcBAb8C6AGZQ1rv_D_wC7uV7nQ</recordid><startdate>19950811</startdate><enddate>19950811</enddate><creator>Hajnóczky, György</creator><creator>Robb-Gaspers, Lawrence D</creator><creator>Seitz, Michele B</creator><creator>Thomas, Andrew P</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19950811</creationdate><title>Decoding of cytosolic calcium oscillations in the mitochondria</title><author>Hajnóczky, György ; Robb-Gaspers, Lawrence D ; Seitz, Michele B ; Thomas, Andrew P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c469t-c5c3eaf5a52e3243b44f1b8fa49453b99e9d64a31982c5cb7813d8a90004d93a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Animals</topic><topic>Calcium - physiology</topic><topic>Liver - physiology</topic><topic>Male</topic><topic>Mitochondria, Liver - physiology</topic><topic>Oxidation-Reduction</topic><topic>Oxidoreductases - physiology</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hajnóczky, György</creatorcontrib><creatorcontrib>Robb-Gaspers, Lawrence D</creatorcontrib><creatorcontrib>Seitz, Michele B</creatorcontrib><creatorcontrib>Thomas, Andrew P</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hajnóczky, György</au><au>Robb-Gaspers, Lawrence D</au><au>Seitz, Michele B</au><au>Thomas, Andrew P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Decoding of cytosolic calcium oscillations in the mitochondria</atitle><jtitle>Cell</jtitle><addtitle>Cell</addtitle><date>1995-08-11</date><risdate>1995</risdate><volume>82</volume><issue>3</issue><spage>415</spage><epage>424</epage><pages>415-424</pages><issn>0092-8674</issn><eissn>1097-4172</eissn><abstract>Frequency-modulated oscillations of cytosolic Ca
2+ ([Ca
2+]
c) are believed to be important in signal transduction, but it has been difficult to correlate [Ca
2+]
c oscillations directly with the activity of Ca
2+-regulated targets. We have studied the control of Ca
2+-sensitive mitochondrial dehydrogenases (CSMDHs) by monitoring mitochondria) Ca
2+ ([Ca
2+]
m) and the redox state of flavoproteins and pyridine nucleotides simultaneously with [Ca
2+]
c in single hepatocytes. Oscillations of [Ca
2+]
c induced by IP
3-dependent hormones were efficiently transmitted to the mitochondria as [Ca
2+]
m oscillations. Each [Ca
2+]
m spike was sufficient to cause a maximal transient activation of the CSMDHs and [Ca
2+]
m oscillations at frequencies above 0.5 per minute caused a sustained activation of mitochondrial metabolism. By contrast, sustained [Ca
2+]
c increases yielded only transient CSMDH activation, and slow or partial [Ca
2+]
c elevations were ineffective in increasing [Ca
2+]
m or stimulating CSMDHs. We conclude that the mitochondria are tuned to oscillating [Ca
2+]
c signals, the frequency of which can control the CSMDHs over the full range of potential activities.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>7634331</pmid><doi>10.1016/0092-8674(95)90430-1</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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language | eng |
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source | MEDLINE; Cell Press Free Archives; ScienceDirect Freedom Collection (Elsevier); Free E-Journal (出版社公開部分のみ) |
subjects | Animals Calcium - physiology Liver - physiology Male Mitochondria, Liver - physiology Oxidation-Reduction Oxidoreductases - physiology Rats Rats, Sprague-Dawley |
title | Decoding of cytosolic calcium oscillations in the mitochondria |
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