Integrative vectors for heterologous gene expression in Streptomyces spp

Integrative expression vectors for heterologous expression of the genes in Streptomyces were developed. The vectors are comprised of a strong constitutive promoter, P E, a synthetic ribosome-binding site, ATG start codon, multiple cloning site, transcription terminator and hygromycin-resistance-enco...

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Bibliographische Detailangaben
Veröffentlicht in:	Gene 1995-07, Vol.160 (1), p.25-31
Hauptverfasser:	Motamedi, Haideh, Shafiee, Ali, Cai, Sheng-Jian
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Bifunctional vector Binding Sites bioconversion Cloning, Molecular - methods Codon Conjugation, Genetic disruption vector DNA Primers Escherichia coli expression cassette FK506 FK506-methyltransferase Gene Expression Genes, Bacterial Genetic Complementation Test Genetic Vectors mini-circle Molecular Sequence Data Polymerase Chain Reaction Promoter Regions, Genetic recombinant strain Restriction Mapping Ribosomes - metabolism Streptomyces Streptomyces - genetics Streptomyces - metabolism Terminator Regions, Genetic Transcription, Genetic
Online-Zugang:	Volltext
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