Construction of Actinobacillus pleuropneumoniae-Escherichia coli shuttle vectors: expression of antibiotic-resistance genes
We constructed several cloning vectors, designated pGZRS-18/19 and pGZRS-38/39, which were based on an endogenous Actinobacillus pleuropneumoniae (Apl) 4.3-kb plasmid. They carry the lacZα-complementation fragment and MCS from pUC18/19, and either the bla gene under the control of a putative Apl pro...
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| Veröffentlicht in: | Gene 1995-07, Vol.160 (1), p.81-86 |
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| creator | West, Susan E.H. Romero, Mary Jo M. Regassa, Laura B. Zielinski, Nicolette A. Welch, Rodney A. |
| description | We constructed several cloning vectors, designated pGZRS-18/19 and pGZRS-38/39, which were based on an endogenous
Actinobacillus pleuropneumoniae (Apl) 4.3-kb plasmid. They carry the
lacZα-complementation fragment and MCS from pUC18/19, and either the bla gene under the control of a putative
Apl promoter or the
Km
R gene from Tn903. These vectors replicate in representative strains of
Apl serotypes 1 and 7,
Escherichia coli,
Pasteurella haemolytica (Ph) and
Haemophilus (Actinobacillus)
actinomycetemcomitans. We also found that
Apl and
Ph did not express genes under the control of the
lacZ or
bla promoters, suggesting that their RNA polymerases may not utilize these
promoters. |
| doi_str_mv | 10.1016/0378-1119(95)00236-Y |
| format | Article |
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Actinobacillus pleuropneumoniae (Apl) 4.3-kb plasmid. They carry the
lacZα-complementation fragment and MCS from pUC18/19, and either the bla gene under the control of a putative
Apl promoter or the
Km
R gene from Tn903. These vectors replicate in representative strains of
Apl serotypes 1 and 7,
Escherichia coli,
Pasteurella haemolytica (Ph) and
Haemophilus (Actinobacillus)
actinomycetemcomitans. We also found that
Apl and
Ph did not express genes under the control of the
lacZ or
bla promoters, suggesting that their RNA polymerases may not utilize these
promoters.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/0378-1119(95)00236-Y</identifier><identifier>PMID: 7628722</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Actinobacillus pleuropneumoniae ; Actinobacillus pleuropneumoniae - genetics ; Anti-Bacterial Agents - pharmacology ; beta-Galactosidase - biosynthesis ; beta-lactamase ; bla gene ; cloning ; Cloning, Molecular - methods ; DNA replication ; drug resistance ; Drug Resistance, Microbial - genetics ; electroporation ; Escherichia coli ; Escherichia coli - genetics ; gene expression ; Genes, Bacterial ; Genetic Complementation Test ; genetic transformation ; Genetic Vectors ; Haemophilus - genetics ; kanamycin ; lacz gene ; Mannheimia haemolytica - genetics ; Pasteurella haemolytica ; Phenotype ; plasmid vectors ; Plasmids ; promoter regions ; Promoter Regions, Genetic ; recombinant DNA ; reporter genes ; Restriction Mapping ; Species Specificity ; streptomycin</subject><ispartof>Gene, 1995-07, Vol.160 (1), p.81-86</ispartof><rights>1995</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c448t-df73b9bc0c8ce1fe5249ad92310b09a3410fde5ed57ac7eb8e6aa919808483f43</citedby><cites>FETCH-LOGICAL-c448t-df73b9bc0c8ce1fe5249ad92310b09a3410fde5ed57ac7eb8e6aa919808483f43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0378-1119(95)00236-Y$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7628722$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>West, Susan E.H.</creatorcontrib><creatorcontrib>Romero, Mary Jo M.</creatorcontrib><creatorcontrib>Regassa, Laura B.</creatorcontrib><creatorcontrib>Zielinski, Nicolette A.</creatorcontrib><creatorcontrib>Welch, Rodney A.</creatorcontrib><title>Construction of Actinobacillus pleuropneumoniae-Escherichia coli shuttle vectors: expression of antibiotic-resistance genes</title><title>Gene</title><addtitle>Gene</addtitle><description>We constructed several cloning vectors, designated pGZRS-18/19 and pGZRS-38/39, which were based on an endogenous
Actinobacillus pleuropneumoniae (Apl) 4.3-kb plasmid. They carry the
lacZα-complementation fragment and MCS from pUC18/19, and either the bla gene under the control of a putative
Apl promoter or the
Km
R gene from Tn903. These vectors replicate in representative strains of
Apl serotypes 1 and 7,
Escherichia coli,
Pasteurella haemolytica (Ph) and
Haemophilus (Actinobacillus)
actinomycetemcomitans. We also found that
Apl and
Ph did not express genes under the control of the
lacZ or
bla promoters, suggesting that their RNA polymerases may not utilize these
promoters.</description><subject>Actinobacillus pleuropneumoniae</subject><subject>Actinobacillus pleuropneumoniae - genetics</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>beta-Galactosidase - biosynthesis</subject><subject>beta-lactamase</subject><subject>bla gene</subject><subject>cloning</subject><subject>Cloning, Molecular - methods</subject><subject>DNA replication</subject><subject>drug resistance</subject><subject>Drug Resistance, Microbial - genetics</subject><subject>electroporation</subject><subject>Escherichia coli</subject><subject>Escherichia coli - genetics</subject><subject>gene expression</subject><subject>Genes, Bacterial</subject><subject>Genetic Complementation Test</subject><subject>genetic transformation</subject><subject>Genetic Vectors</subject><subject>Haemophilus - genetics</subject><subject>kanamycin</subject><subject>lacz gene</subject><subject>Mannheimia haemolytica - genetics</subject><subject>Pasteurella haemolytica</subject><subject>Phenotype</subject><subject>plasmid vectors</subject><subject>Plasmids</subject><subject>promoter regions</subject><subject>Promoter Regions, Genetic</subject><subject>recombinant DNA</subject><subject>reporter genes</subject><subject>Restriction Mapping</subject><subject>Species Specificity</subject><subject>streptomycin</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFv1DAQhS0EKtvCPwCREyqHFDuOY7sHpGpVoFIlDtBDT5bjTLpGWTt4nIqKP4-XXfVY5uKR55un0XuEvGH0jFHWfaRcqpoxpk-1-EBpw7v69hlZMSV1TSlXz8nqEXlJjhF_0lJCNEfkSHaNkk2zIn_WMWBOi8s-hiqO1UXpQuyt89O0YDVPsKQ4B1i2MXgL9SW6DSTvNt5WLk6-ws2S8wTVPbgcE55X8HtOgHjQsyH73sfsXV1-PWYbHFR3EABfkRejnRBeH94TcvP58sf6a3397cvV-uK6dm2rcj2Mkve6d9QpB2wE0bTaDrrhjPZUW94yOg4gYBDSOgm9gs5azbSiqlV8bPkJeb_XnVP8tQBms_XoYJpsgLigkbLlVDLxX5B1SmvFZQHbPehSREwwmjn5rU0PhlGzC8fsnDc7540W5l845rasvT3oL_0WhselQxpl_m4_H2009i55NDffG8o4ZYJ2SulCfNoTUPy695AMOg_F0cGn4r8Zon_6hL-e8qt0</recordid><startdate>19950704</startdate><enddate>19950704</enddate><creator>West, Susan E.H.</creator><creator>Romero, Mary Jo M.</creator><creator>Regassa, Laura B.</creator><creator>Zielinski, Nicolette A.</creator><creator>Welch, Rodney A.</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>19950704</creationdate><title>Construction of Actinobacillus pleuropneumoniae-Escherichia coli shuttle vectors: expression of antibiotic-resistance genes</title><author>West, Susan E.H. ; Romero, Mary Jo M. ; Regassa, Laura B. ; Zielinski, Nicolette A. ; Welch, Rodney A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c448t-df73b9bc0c8ce1fe5249ad92310b09a3410fde5ed57ac7eb8e6aa919808483f43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Actinobacillus pleuropneumoniae</topic><topic>Actinobacillus pleuropneumoniae - genetics</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>beta-Galactosidase - biosynthesis</topic><topic>beta-lactamase</topic><topic>bla gene</topic><topic>cloning</topic><topic>Cloning, Molecular - methods</topic><topic>DNA replication</topic><topic>drug resistance</topic><topic>Drug Resistance, Microbial - genetics</topic><topic>electroporation</topic><topic>Escherichia coli</topic><topic>Escherichia coli - genetics</topic><topic>gene expression</topic><topic>Genes, Bacterial</topic><topic>Genetic Complementation Test</topic><topic>genetic transformation</topic><topic>Genetic Vectors</topic><topic>Haemophilus - genetics</topic><topic>kanamycin</topic><topic>lacz gene</topic><topic>Mannheimia haemolytica - genetics</topic><topic>Pasteurella haemolytica</topic><topic>Phenotype</topic><topic>plasmid vectors</topic><topic>Plasmids</topic><topic>promoter regions</topic><topic>Promoter Regions, Genetic</topic><topic>recombinant DNA</topic><topic>reporter genes</topic><topic>Restriction Mapping</topic><topic>Species Specificity</topic><topic>streptomycin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>West, Susan E.H.</creatorcontrib><creatorcontrib>Romero, Mary Jo M.</creatorcontrib><creatorcontrib>Regassa, Laura B.</creatorcontrib><creatorcontrib>Zielinski, Nicolette A.</creatorcontrib><creatorcontrib>Welch, Rodney A.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>West, Susan E.H.</au><au>Romero, Mary Jo M.</au><au>Regassa, Laura B.</au><au>Zielinski, Nicolette A.</au><au>Welch, Rodney A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Construction of Actinobacillus pleuropneumoniae-Escherichia coli shuttle vectors: expression of antibiotic-resistance genes</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>1995-07-04</date><risdate>1995</risdate><volume>160</volume><issue>1</issue><spage>81</spage><epage>86</epage><pages>81-86</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><abstract>We constructed several cloning vectors, designated pGZRS-18/19 and pGZRS-38/39, which were based on an endogenous
Actinobacillus pleuropneumoniae (Apl) 4.3-kb plasmid. They carry the
lacZα-complementation fragment and MCS from pUC18/19, and either the bla gene under the control of a putative
Apl promoter or the
Km
R gene from Tn903. These vectors replicate in representative strains of
Apl serotypes 1 and 7,
Escherichia coli,
Pasteurella haemolytica (Ph) and
Haemophilus (Actinobacillus)
actinomycetemcomitans. We also found that
Apl and
Ph did not express genes under the control of the
lacZ or
bla promoters, suggesting that their RNA polymerases may not utilize these
promoters.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>7628722</pmid><doi>10.1016/0378-1119(95)00236-Y</doi><tpages>6</tpages></addata></record> |
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| ispartof | Gene, 1995-07, Vol.160 (1), p.81-86 |
| issn | 0378-1119 1879-0038 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_77430715 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Actinobacillus pleuropneumoniae Actinobacillus pleuropneumoniae - genetics Anti-Bacterial Agents - pharmacology beta-Galactosidase - biosynthesis beta-lactamase bla gene cloning Cloning, Molecular - methods DNA replication drug resistance Drug Resistance, Microbial - genetics electroporation Escherichia coli Escherichia coli - genetics gene expression Genes, Bacterial Genetic Complementation Test genetic transformation Genetic Vectors Haemophilus - genetics kanamycin lacz gene Mannheimia haemolytica - genetics Pasteurella haemolytica Phenotype plasmid vectors Plasmids promoter regions Promoter Regions, Genetic recombinant DNA reporter genes Restriction Mapping Species Specificity streptomycin |
| title | Construction of Actinobacillus pleuropneumoniae-Escherichia coli shuttle vectors: expression of antibiotic-resistance genes |
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