Guanidine-dependent mutants of poliovirus: Identification of three classes with different growth requirements
Four mutants resistant to high (2.0 m M) guanidine were derived from a mutant resistant to intermediate (0.53 m M) levels of this drug. One of these mutants was found to be resistant to high guanidine and was shown to contain a mutation within 2C seen previously in this class of mutants, while lacki...
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| Veröffentlicht in: | Virology (New York, N.Y.) N.Y.), 1987-03, Vol.157 (1), p.83-88 |
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| container_title | Virology (New York, N.Y.) |
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| creator | Pincus, Steven E. Rohl, Holger Wimmer, Eckard |
| description | Four mutants resistant to high (2.0 m
M) guanidine were derived from a mutant resistant to intermediate (0.53 m
M) levels of this drug. One of these mutants was found to be resistant to high guanidine and was shown to contain a mutation within 2C seen previously in this class of mutants, while lacking the mutation seen in the intermediate parent. The other three mutants were dependent on guanidine for growth and contained the mutation in 2C seen in the parental virus as well as a mutation seen previously in another dependent mutant. Comparison of the newly isolated dependent mutants to two previously described dependent mutants revealed that three classes of dependent mutants which vary in their requirements for optimal growth can be observed. We present a model for the interaction of guanidine with 2C that explains the occurrence of the three classes of dependent mutants. |
| doi_str_mv | 10.1016/0042-6822(87)90316-3 |
| format | Article |
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M) guanidine were derived from a mutant resistant to intermediate (0.53 m
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M) guanidine were derived from a mutant resistant to intermediate (0.53 m
M) levels of this drug. One of these mutants was found to be resistant to high guanidine and was shown to contain a mutation within 2C seen previously in this class of mutants, while lacking the mutation seen in the intermediate parent. The other three mutants were dependent on guanidine for growth and contained the mutation in 2C seen in the parental virus as well as a mutation seen previously in another dependent mutant. Comparison of the newly isolated dependent mutants to two previously described dependent mutants revealed that three classes of dependent mutants which vary in their requirements for optimal growth can be observed. We present a model for the interaction of guanidine with 2C that explains the occurrence of the three classes of dependent mutants.</description><subject>Biological and medical sciences</subject><subject>Drug Resistance, Microbial</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes</subject><subject>Genes, Viral</subject><subject>Genetics</subject><subject>Guanidine</subject><subject>Guanidines - pharmacology</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Microbiology</subject><subject>Mutation</subject><subject>poliovirus</subject><subject>Poliovirus - drug effects</subject><subject>Poliovirus - genetics</subject><subject>Poliovirus - growth & development</subject><subject>Viral Proteins - genetics</subject><subject>Virology</subject><issn>0042-6822</issn><issn>1096-0341</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU-LFDEQxYMo67j6DRT6IKKH1vybdGcPgiy6Lix40XPIVCpupDuZTdK7-O1NO8Mc9VRUvV89ileEvGT0PaNMfaBU8l6NnL8dh3eaCqZ68YhsGNWqp0Kyx2RzQp6SZ6X8oq0fBnpGzgTlWiu9IfPVYmNwIWLvcI_RYazdvFQba-mS7_ZpCuk-5KVcdNerGHwAW0OKq1pvM2IHky0FS_cQ6m3ngveYV5efOT20Qca7JWSc26g8J0-8nQq-ONZz8uPL5--XX_ubb1fXl59uehCC15773RYQJI4a_M5ttbSt0eCsEnx0HpjQFO1WD-PWg7YOpJWjEDslwHrNxTl5c_Dd53S3YKlmDgVwmmzEtBQzDJIPStL_gkwqOTaugfIAQk6lZPRmn8Ns82_DqFnfYdaszZq1GQfz9x1mXXt19F92M7rT0jH_pr8-6raAnXy2EUI5Yc2Ni4E17OMBwxbafcBsCgSMgK4lC9W4FP59xx8PxKlb</recordid><startdate>198703</startdate><enddate>198703</enddate><creator>Pincus, Steven E.</creator><creator>Rohl, Holger</creator><creator>Wimmer, Eckard</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>198703</creationdate><title>Guanidine-dependent mutants of poliovirus: Identification of three classes with different growth requirements</title><author>Pincus, Steven E. ; Rohl, Holger ; Wimmer, Eckard</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c332t-2fb5cec4e89cfbd594ac4e9cda6328dfc1390ea59785fc9adc4a4833b63caf923</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Biological and medical sciences</topic><topic>Drug Resistance, Microbial</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes</topic><topic>Genes, Viral</topic><topic>Genetics</topic><topic>Guanidine</topic><topic>Guanidines - pharmacology</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Microbiology</topic><topic>Mutation</topic><topic>poliovirus</topic><topic>Poliovirus - drug effects</topic><topic>Poliovirus - genetics</topic><topic>Poliovirus - growth & development</topic><topic>Viral Proteins - genetics</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pincus, Steven E.</creatorcontrib><creatorcontrib>Rohl, Holger</creatorcontrib><creatorcontrib>Wimmer, Eckard</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Virology (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pincus, Steven E.</au><au>Rohl, Holger</au><au>Wimmer, Eckard</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Guanidine-dependent mutants of poliovirus: Identification of three classes with different growth requirements</atitle><jtitle>Virology (New York, N.Y.)</jtitle><addtitle>Virology</addtitle><date>1987-03</date><risdate>1987</risdate><volume>157</volume><issue>1</issue><spage>83</spage><epage>88</epage><pages>83-88</pages><issn>0042-6822</issn><eissn>1096-0341</eissn><coden>VIRLAX</coden><abstract>Four mutants resistant to high (2.0 m
M) guanidine were derived from a mutant resistant to intermediate (0.53 m
M) levels of this drug. One of these mutants was found to be resistant to high guanidine and was shown to contain a mutation within 2C seen previously in this class of mutants, while lacking the mutation seen in the intermediate parent. The other three mutants were dependent on guanidine for growth and contained the mutation in 2C seen in the parental virus as well as a mutation seen previously in another dependent mutant. Comparison of the newly isolated dependent mutants to two previously described dependent mutants revealed that three classes of dependent mutants which vary in their requirements for optimal growth can be observed. We present a model for the interaction of guanidine with 2C that explains the occurrence of the three classes of dependent mutants.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>3029969</pmid><doi>10.1016/0042-6822(87)90316-3</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0042-6822 |
| ispartof | Virology (New York, N.Y.), 1987-03, Vol.157 (1), p.83-88 |
| issn | 0042-6822 1096-0341 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_77427640 |
| source | MEDLINE; Access via ScienceDirect (Elsevier); EZB-FREE-00999 freely available EZB journals |
| subjects | Biological and medical sciences Drug Resistance, Microbial Fundamental and applied biological sciences. Psychology Genes Genes, Viral Genetics Guanidine Guanidines - pharmacology HeLa Cells Humans Microbiology Mutation poliovirus Poliovirus - drug effects Poliovirus - genetics Poliovirus - growth & development Viral Proteins - genetics Virology |
| title | Guanidine-dependent mutants of poliovirus: Identification of three classes with different growth requirements |
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