Enhanced transglycosylation activity of Arthrobacter protophormiae endo-beta-N-acetylglucosaminidase in media containing organic solvents

The transglycosylation activity of endo-beta-N-acetylglucosaminidase from Arthrobacter protophormiae (endo-A) was enhanced by inclusion of organic solvents in the reaction mixture. In aqueous solution, the transglycosylation yield relative to starting substrate was 32% using Man9GlcNAc2Asn as donor...

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Veröffentlicht in:	The Journal of biological chemistry 1995-07, Vol.270 (30), p.17723-17729
Hauptverfasser:	Fan, J Q, Takegawa, K, Iwahara, S, Kondo, A, Kato, I, Abeygunawardana, C, Lee, Y C
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetylglucosamine - metabolism Acetylglucosaminidase - metabolism Arthrobacter - enzymology Carbohydrate Sequence Glycosylation Hydrolysis Kinetics Magnetic Resonance Spectroscopy Mass Spectrometry - methods Molecular Sequence Data Monosaccharides - chemistry Protein Conformation Solutions Solvents
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