Identification of YHR068w in Saccharomyces cerevisiae chromosome VIII as a gene for deoxyhypusine synthase: expression and characterization of the enzyme

Deoxyhypusine synthase catalyzes the formation of deoxyhypusine, the first step in hypusine biosynthesis. Amino acid sequences of five tryptic peptides from rat deoxyhypusine synthase were found to match partially the deduced amino acid sequence of the open reading frame of gene YHR068w of Saccharom...

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Veröffentlicht in:	The Journal of biological chemistry 1995-08, Vol.270 (31), p.18408-18412
Hauptverfasser:	Kang, K.R. (National Institutes of Health, Bethesda, MD.), Wolff, E.C, Park, M.H, Folk, J.E, Chung, S.I
Format:	Artikel
Sprache:	eng
Schlagworte:	ACTIVIDAD ENZIMATICA ACTIVITE ENZYMATIQUE ADN Amino Acid Sequence Animals Base Sequence Chromosomes, Fungal - genetics COMPOSICION QUIMICA COMPOSITION CHIMIQUE DNA, Complementary - genetics ESCHERICHIA COLI Escherichia coli - genetics Eukaryotic Translation Initiation Factor 5A EXPRESION GENICA EXPRESSION DES GENES Fungal Proteins - biosynthesis Fungal Proteins - genetics GENE GENES Genes, Fungal - genetics Humans Lysine - analogs & derivatives Lysine - biosynthesis Male Molecular Sequence Data Molecular Weight Oxidoreductases Acting on CH-NH Group Donors - biosynthesis Oxidoreductases Acting on CH-NH Group Donors - genetics Oxidoreductases Acting on CH-NH Group Donors - isolation & purification Oxidoreductases Acting on CH-NH Group Donors - metabolism Peptide Initiation Factors - metabolism Polymerase Chain Reaction PURIFICACION PURIFICATION Rats Recombinant Proteins - biosynthesis Recombinant Proteins - isolation & purification Recombinant Proteins - metabolism RNA-Binding Proteins SACCHAROMYCES CEREVISIAE Saccharomyces cerevisiae - enzymology Saccharomyces cerevisiae - genetics SECUENCIA NUCLEOTIDICA Sequence Homology, Amino Acid SEQUENCE NUCLEOTIDIQUE Testis - metabolism TRANSFORMACION GENETICA TRANSFORMATION GENETIQUE
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