Prevalence of trimethoprim resistant dihydrofolate reductase genes identified with oligonucleotide probes in plasmids from isolates of commensal faecal flora
In a recent survey of trimethoprim resistance, 357 Gram-negative aerobic organisms were isolated from healthy volunteers from rural and urban populations in South Africa. Trimethoprim resistance was transferable in 184 (52%) of the isolates. A further 12 (3%) transferred in the presence of an X4 fac...
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| Veröffentlicht in: | Journal of antimicrobial chemotherapy 1995-04, Vol.35 (4), p.497-508 |
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| creator | Adrian, P. V. Klugman, K. P. Amyes, S. G. B. |
| description | In a recent survey of trimethoprim resistance, 357 Gram-negative aerobic organisms were isolated from healthy volunteers from rural and urban populations in South Africa. Trimethoprim resistance was transferable in 184 (52%) of the isolates. A further 12 (3%) transferred in the presence of an X4 factor. The transconjugants were probed with intragenic oligonucleotide probes for the type la, Ib, IlIa, VIII, V, VI, VII, IX, X and XII dihydrofolate reductase genes. Contrary to all previous data, the most prevalent dihydrofolate reductase gene was the type Ib (30%) followedby the type VIII (23%), V (13%), la (6%), VII (3%) and XII (0.5%). None of the strains hybridised to the type IlIa, XI, X and the VI dihydrofolate reductase probes. Plasmid restriction profiles revealed that the high prevalence of the type Ib and VIII dihydrofolate reductase genes resulted from the presence of ubiquitous plasmids. These results highlight the previous problems associated with the distinction of closely related dihydrofolate reductasegenes. |
| doi_str_mv | 10.1093/jac/35.4.497 |
| format | Article |
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V. ; Klugman, K. P. ; Amyes, S. G. B.</creator><creatorcontrib>Adrian, P. V. ; Klugman, K. P. ; Amyes, S. G. B.</creatorcontrib><description>In a recent survey of trimethoprim resistance, 357 Gram-negative aerobic organisms were isolated from healthy volunteers from rural and urban populations in South Africa. Trimethoprim resistance was transferable in 184 (52%) of the isolates. A further 12 (3%) transferred in the presence of an X4 factor. The transconjugants were probed with intragenic oligonucleotide probes for the type la, Ib, IlIa, VIII, V, VI, VII, IX, X and XII dihydrofolate reductase genes. Contrary to all previous data, the most prevalent dihydrofolate reductase gene was the type Ib (30%) followedby the type VIII (23%), V (13%), la (6%), VII (3%) and XII (0.5%). None of the strains hybridised to the type IlIa, XI, X and the VI dihydrofolate reductase probes. Plasmid restriction profiles revealed that the high prevalence of the type Ib and VIII dihydrofolate reductase genes resulted from the presence of ubiquitous plasmids. These results highlight the previous problems associated with the distinction of closely related dihydrofolate reductasegenes.</description><identifier>ISSN: 0305-7453</identifier><identifier>EISSN: 1460-2091</identifier><identifier>DOI: 10.1093/jac/35.4.497</identifier><identifier>PMID: 7628984</identifier><identifier>CODEN: JACHDX</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Antibacterial agents ; Antibiotics. Antiinfectious agents. 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Drug treatments ; Plasmids ; South Africa ; Tetrahydrofolate Dehydrogenase - genetics ; Transformation, Bacterial ; Trimethoprim Resistance - genetics</subject><ispartof>Journal of antimicrobial chemotherapy, 1995-04, Vol.35 (4), p.497-508</ispartof><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c384t-9cac53eecbe8a2c7b63ef0ea31261ce0b0ea12098135584f0cbecd557a03ce913</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3493348$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7628984$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Adrian, P. V.</creatorcontrib><creatorcontrib>Klugman, K. P.</creatorcontrib><creatorcontrib>Amyes, S. G. B.</creatorcontrib><title>Prevalence of trimethoprim resistant dihydrofolate reductase genes identified with oligonucleotide probes in plasmids from isolates of commensal faecal flora</title><title>Journal of antimicrobial chemotherapy</title><addtitle>J Antimicrob Chemother</addtitle><description>In a recent survey of trimethoprim resistance, 357 Gram-negative aerobic organisms were isolated from healthy volunteers from rural and urban populations in South Africa. Trimethoprim resistance was transferable in 184 (52%) of the isolates. A further 12 (3%) transferred in the presence of an X4 factor. The transconjugants were probed with intragenic oligonucleotide probes for the type la, Ib, IlIa, VIII, V, VI, VII, IX, X and XII dihydrofolate reductase genes. Contrary to all previous data, the most prevalent dihydrofolate reductase gene was the type Ib (30%) followedby the type VIII (23%), V (13%), la (6%), VII (3%) and XII (0.5%). None of the strains hybridised to the type IlIa, XI, X and the VI dihydrofolate reductase probes. Plasmid restriction profiles revealed that the high prevalence of the type Ib and VIII dihydrofolate reductase genes resulted from the presence of ubiquitous plasmids. These results highlight the previous problems associated with the distinction of closely related dihydrofolate reductasegenes.</description><subject>Antibacterial agents</subject><subject>Antibiotics. Antiinfectious agents. Antiparasitic agents</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Conjugation, Genetic</subject><subject>DNA Restriction Enzymes</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli - metabolism</subject><subject>Feces - microbiology</subject><subject>Gram-Negative Aerobic Bacteria</subject><subject>Humans</subject><subject>In Situ Hybridization</subject><subject>Medical sciences</subject><subject>Molecular Sequence Data</subject><subject>Oligonucleotide Probes</subject><subject>Pharmacology. Drug treatments</subject><subject>Plasmids</subject><subject>South Africa</subject><subject>Tetrahydrofolate Dehydrogenase - genetics</subject><subject>Transformation, Bacterial</subject><subject>Trimethoprim Resistance - genetics</subject><issn>0305-7453</issn><issn>1460-2091</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU2LFDEYhBtR1nH15lXIQTzZs0nns48yqCss-IGi7CVk0m92sqY7Y5JW98f4X007w1w9VUg9VEJV0zwleE1wTy9ujb2gfM3WrJf3mhVhArcd7sn9ZoUp5q1knD5sHuV8izEWXKiz5kyKTvWKrZo_HxL8NAEmCyg6VJIfoezivipKkH0uZipo8Lu7IUUXgylQ74fZFpMB3cAEGfkBpuKdhwH98mWHYvA3cZptgFiqh_YpbhdsQvtg8uiHjFyKI_L5X15eHrZxHGHKJiBnwC4SYjKPmwfOhAxPjnrefHnz-vPmsr16__bd5tVVa6lipe2tsZwC2C0o01m5FRQcBkNJJ4gFvK1nUitRhHKumMMVtAPn0mBqoSf0vHlxyK1f_TFDLnr02UIIZoI4Zy0l6zhm3X9BIhTBRIgKvjyANsWcEzi9VGrSnSZYL7PpOpumXDNdZ6v4s2PuvB1hOMHHnar__OibXNtxyUzW5xNGWU8pUxVrD1jdDX6fbJO-ayGp5Pry27WW_NNH_PVa6Q39C6pmtJY</recordid><startdate>19950401</startdate><enddate>19950401</enddate><creator>Adrian, P. V.</creator><creator>Klugman, K. P.</creator><creator>Amyes, S. G. B.</creator><general>Oxford University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>19950401</creationdate><title>Prevalence of trimethoprim resistant dihydrofolate reductase genes identified with oligonucleotide probes in plasmids from isolates of commensal faecal flora</title><author>Adrian, P. V. ; Klugman, K. P. ; Amyes, S. G. B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c384t-9cac53eecbe8a2c7b63ef0ea31261ce0b0ea12098135584f0cbecd557a03ce913</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Antibacterial agents</topic><topic>Antibiotics. Antiinfectious agents. Antiparasitic agents</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Conjugation, Genetic</topic><topic>DNA Restriction Enzymes</topic><topic>Escherichia coli - genetics</topic><topic>Escherichia coli - metabolism</topic><topic>Feces - microbiology</topic><topic>Gram-Negative Aerobic Bacteria</topic><topic>Humans</topic><topic>In Situ Hybridization</topic><topic>Medical sciences</topic><topic>Molecular Sequence Data</topic><topic>Oligonucleotide Probes</topic><topic>Pharmacology. Drug treatments</topic><topic>Plasmids</topic><topic>South Africa</topic><topic>Tetrahydrofolate Dehydrogenase - genetics</topic><topic>Transformation, Bacterial</topic><topic>Trimethoprim Resistance - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Adrian, P. V.</creatorcontrib><creatorcontrib>Klugman, K. P.</creatorcontrib><creatorcontrib>Amyes, S. G. 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B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Prevalence of trimethoprim resistant dihydrofolate reductase genes identified with oligonucleotide probes in plasmids from isolates of commensal faecal flora</atitle><jtitle>Journal of antimicrobial chemotherapy</jtitle><addtitle>J Antimicrob Chemother</addtitle><date>1995-04-01</date><risdate>1995</risdate><volume>35</volume><issue>4</issue><spage>497</spage><epage>508</epage><pages>497-508</pages><issn>0305-7453</issn><eissn>1460-2091</eissn><coden>JACHDX</coden><abstract>In a recent survey of trimethoprim resistance, 357 Gram-negative aerobic organisms were isolated from healthy volunteers from rural and urban populations in South Africa. Trimethoprim resistance was transferable in 184 (52%) of the isolates. A further 12 (3%) transferred in the presence of an X4 factor. The transconjugants were probed with intragenic oligonucleotide probes for the type la, Ib, IlIa, VIII, V, VI, VII, IX, X and XII dihydrofolate reductase genes. Contrary to all previous data, the most prevalent dihydrofolate reductase gene was the type Ib (30%) followedby the type VIII (23%), V (13%), la (6%), VII (3%) and XII (0.5%). None of the strains hybridised to the type IlIa, XI, X and the VI dihydrofolate reductase probes. Plasmid restriction profiles revealed that the high prevalence of the type Ib and VIII dihydrofolate reductase genes resulted from the presence of ubiquitous plasmids. These results highlight the previous problems associated with the distinction of closely related dihydrofolate reductasegenes.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>7628984</pmid><doi>10.1093/jac/35.4.497</doi><tpages>12</tpages></addata></record> |
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| ispartof | Journal of antimicrobial chemotherapy, 1995-04, Vol.35 (4), p.497-508 |
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| subjects | Antibacterial agents Antibiotics. Antiinfectious agents. Antiparasitic agents Base Sequence Biological and medical sciences Blotting, Western Conjugation, Genetic DNA Restriction Enzymes Escherichia coli - genetics Escherichia coli - metabolism Feces - microbiology Gram-Negative Aerobic Bacteria Humans In Situ Hybridization Medical sciences Molecular Sequence Data Oligonucleotide Probes Pharmacology. Drug treatments Plasmids South Africa Tetrahydrofolate Dehydrogenase - genetics Transformation, Bacterial Trimethoprim Resistance - genetics |
| title | Prevalence of trimethoprim resistant dihydrofolate reductase genes identified with oligonucleotide probes in plasmids from isolates of commensal faecal flora |
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