Whole-Genome Random Sequencing and Assembly of Haemophilus Influenzae Rd

An approach for genome analysis based on sequencing and assembly of unselected pieces of DNA from the whole chromosome has been applied to obtain the complete nucleotide sequence (1,830, 137 base pairs) of the genome from the bacterium Haemophilus influenzae Rd. This approach eliminates the need for...

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Veröffentlicht in:	Science 1995-07, Vol.269 (5223), p.496-512
Hauptverfasser:	Fleischmann, Robert D., Adams, Mark D., White, Owen, Clayton, Rebecca A., Kirkness, Ewen F., Kerlavage, Anthony R., Bult, Carol J., Tomb, Jean-Francois, Dougherty, Brian A., Merrick, Joseph M., McKenney, Keith, Sutton, Granger, FitzHugh, Will, Fields, Chris, Gocyne, Jeannie D., Scott, John, Shirley, Robert, Liu, Li-Ing, Glodek, Anna, Kelley, Jenny M., Weidman, Janice F., Phillips, Cheryl A., Spriggs, Tracy, Hedblom, Eva, Cotton, Matthew D., Utterback, Teresa R., Hanna, Michael C., Nguyen, David T., Saudek, Deborah M., Brandon, Rhonda C., Fine, Leah D., Fritchman, Janice L., Fuhrmann, Joyce L., Geoghagen, N. S. M., Gnehm, Cheryl L., McDonald, Lisa A., Small, Keith V., Fraser, Claire M., Smith, Hamilton O., Venter, J. Craig
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino acids Bacteria Bacterial Proteins - genetics Bacteriology Base Composition Base Sequence Biological and medical sciences BIOLOGY AND MEDICINE, BASIC STUDIES Chromosome mapping Chromosome Mapping - methods Chromosomes, Bacterial Cloning, Molecular Costs and Cost Analysis Databases, Factual DNA DNA, Bacterial - genetics Fundamental and applied biological sciences. Psychology Genes, Bacterial Genetic aspects GENETIC MAPPING Genetics Genome, Bacterial Genomes HAEMOPHILUS Haemophilus influenzae Haemophilus influenzae - genetics Haemophilus influenzae - physiology Hemophilus influenzae Libraries Membrane transport proteins Microbiology Molecular Sequence Data Nuclear membrane Operator regions Operon Pilot Projects Repetitive Sequences, Nucleic Acid Research Article RESEARCH PROGRAMS RNA, Bacterial - genetics RNA, Ribosomal - genetics Sequence Analysis, DNA - methods Sequencing Software Sperm transport Terminator regions
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Beschreibung
Zusammenfassung:	An approach for genome analysis based on sequencing and assembly of unselected pieces of DNA from the whole chromosome has been applied to obtain the complete nucleotide sequence (1,830, 137 base pairs) of the genome from the bacterium Haemophilus influenzae Rd. This approach eliminates the need for initial mapping efforts and is therefore applicable to the vast array of microbial species for which genome maps are unavailable. The H. influenzae Rd genome sequence (Genome Sequence DataBase accession number L42023) represents the only complete genome sequence from a free-living organism.
ISSN:	0036-8075 1095-9203
DOI:	10.1126/science.7542800