Increase in synaptic vesicle proteins in synapsin-transfected NG108-15 cells: A subcellular fractionation study
We have examined the subcellular distribution of synapsins and synaptophysin in density gradients from synapsin‐ and vector‐transfected NGlO8‐15 cells, since we recently found that transfection of synapsin IIb cDNA into neuroblastoma X glioma hybrid cells (NG108‐15) resulted in cell lines that had a...
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| Veröffentlicht in: | Synapse (New York, N.Y.) N.Y.), 1995-05, Vol.20 (1), p.44-53 |
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| creator | Fried, Gabriel Han, Hui-Quan |
| description | We have examined the subcellular distribution of synapsins and synaptophysin in density gradients from synapsin‐ and vector‐transfected NGlO8‐15 cells, since we recently found that transfection of synapsin IIb cDNA into neuroblastoma X glioma hybrid cells (NG108‐15) resulted in cell lines that had a more neuronal phenotype than controls. The increase in synapsins and synaptophysin in the transfected cells was maximal in the region of the gradient containing small synaptic vesicles. The transferrin receptor, a marker for early endosomes, did not increase in the synapsin‐containing fractions in the transfected cells. Secretogranin I, a soluble protein stored in and secreted from large dense cored vesicles, showed a very pronounced increase in the dense regions of gradients from transfected cells. These subcellular fractionation data suggest a possible role for the synapsins in the regulation of synaptic vesicle function. © 1995 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/syn.890200108 |
| format | Article |
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The increase in synapsins and synaptophysin in the transfected cells was maximal in the region of the gradient containing small synaptic vesicles. The transferrin receptor, a marker for early endosomes, did not increase in the synapsin‐containing fractions in the transfected cells. Secretogranin I, a soluble protein stored in and secreted from large dense cored vesicles, showed a very pronounced increase in the dense regions of gradients from transfected cells. 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The increase in synapsins and synaptophysin in the transfected cells was maximal in the region of the gradient containing small synaptic vesicles. The transferrin receptor, a marker for early endosomes, did not increase in the synapsin‐containing fractions in the transfected cells. Secretogranin I, a soluble protein stored in and secreted from large dense cored vesicles, showed a very pronounced increase in the dense regions of gradients from transfected cells. These subcellular fractionation data suggest a possible role for the synapsins in the regulation of synaptic vesicle function. © 1995 Wiley‐Liss, Inc.</description><subject>Animals</subject><subject>Autoradiography</subject><subject>Centrifugation, Density Gradient</subject><subject>DNA - biosynthesis</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Endosomes</subject><subject>Immunoblotting</subject><subject>Immunohistochemistry</subject><subject>Microscopy, Immunoelectron</subject><subject>Nerve Tissue Proteins - metabolism</subject><subject>Neuroblastoma - metabolism</subject><subject>Rats</subject><subject>Rodentia</subject><subject>Secretogranin</subject><subject>Subcellular Fractions - metabolism</subject><subject>Synapsins - metabolism</subject><subject>Synaptic vesicles</subject><subject>Synaptic Vesicles - metabolism</subject><subject>Synaptic Vesicles - ultrastructure</subject><subject>Synaptophysin</subject><subject>Transfection</subject><subject>Transferrin receptor</subject><subject>Tumor Cells, Cultured</subject><issn>0887-4476</issn><issn>1098-2396</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9v0zAUxy0EGt3GkSOST9yyPf-KbW5j2rpJVadpRQguluM4kiFNil8C639PqlbVTnCxLX0__vj5PULeM7hgAPwSt92FscABGJhXZMbAmoILW74mMzBGF1Lq8i05RfwBAIKBPCEnuuTScDsj_X0XcvQYaeropPKbIQX6O2IKbaSb3A8xdXgMMXXFkH2HTQxDrOlyPj1aMEVDbFv8RK8ojtXuPLY-0yb7MKS-87uF4jDW23PypvEtxneH_Yx8ub1ZXd8Vi4f5_fXVoggSlClCLL2pGNe14KqSwmuplda81qCgUcEY7is7hY0STCofqjqUoolVZayprRdn5OPeO33h1xhxcOuEu8J8F_sRndaSGQHyvyArdQncmAks9mDIPWKOjdvktPZ56xi43STc1CF3nMTEfziIx2od6yN9aP2U633-J7Vx-2-Ze_q2fGk-VJJwiM_Hmz7_dKUWWrmvy7mzK_u4-G4_u5X4C0b3pI4</recordid><startdate>199505</startdate><enddate>199505</enddate><creator>Fried, Gabriel</creator><creator>Han, Hui-Quan</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>199505</creationdate><title>Increase in synaptic vesicle proteins in synapsin-transfected NG108-15 cells: A subcellular fractionation study</title><author>Fried, Gabriel ; Han, Hui-Quan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4058-ce6a8b127d325b43a7475772d7050f5c882ab9d32f53145acbdc63febb898d9a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Animals</topic><topic>Autoradiography</topic><topic>Centrifugation, Density Gradient</topic><topic>DNA - biosynthesis</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Endosomes</topic><topic>Immunoblotting</topic><topic>Immunohistochemistry</topic><topic>Microscopy, Immunoelectron</topic><topic>Nerve Tissue Proteins - metabolism</topic><topic>Neuroblastoma - metabolism</topic><topic>Rats</topic><topic>Rodentia</topic><topic>Secretogranin</topic><topic>Subcellular Fractions - metabolism</topic><topic>Synapsins - metabolism</topic><topic>Synaptic vesicles</topic><topic>Synaptic Vesicles - metabolism</topic><topic>Synaptic Vesicles - ultrastructure</topic><topic>Synaptophysin</topic><topic>Transfection</topic><topic>Transferrin receptor</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fried, Gabriel</creatorcontrib><creatorcontrib>Han, Hui-Quan</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Synapse (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fried, Gabriel</au><au>Han, Hui-Quan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Increase in synaptic vesicle proteins in synapsin-transfected NG108-15 cells: A subcellular fractionation study</atitle><jtitle>Synapse (New York, N.Y.)</jtitle><addtitle>Synapse</addtitle><date>1995-05</date><risdate>1995</risdate><volume>20</volume><issue>1</issue><spage>44</spage><epage>53</epage><pages>44-53</pages><issn>0887-4476</issn><eissn>1098-2396</eissn><abstract>We have examined the subcellular distribution of synapsins and synaptophysin in density gradients from synapsin‐ and vector‐transfected NGlO8‐15 cells, since we recently found that transfection of synapsin IIb cDNA into neuroblastoma X glioma hybrid cells (NG108‐15) resulted in cell lines that had a more neuronal phenotype than controls. 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| identifier | ISSN: 0887-4476 |
| ispartof | Synapse (New York, N.Y.), 1995-05, Vol.20 (1), p.44-53 |
| issn | 0887-4476 1098-2396 |
| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals Autoradiography Centrifugation, Density Gradient DNA - biosynthesis Electrophoresis, Polyacrylamide Gel Endosomes Immunoblotting Immunohistochemistry Microscopy, Immunoelectron Nerve Tissue Proteins - metabolism Neuroblastoma - metabolism Rats Rodentia Secretogranin Subcellular Fractions - metabolism Synapsins - metabolism Synaptic vesicles Synaptic Vesicles - metabolism Synaptic Vesicles - ultrastructure Synaptophysin Transfection Transferrin receptor Tumor Cells, Cultured |
| title | Increase in synaptic vesicle proteins in synapsin-transfected NG108-15 cells: A subcellular fractionation study |
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