Cell type-oriented differential modulatory actions of saikosaponin-d on growth responses and DNA fragmentation of lymphocytes triggered by receptor-mediated and receptor-bypassed pathways
We examined the immunoregulatory action of saikosaponin-d (SSd), which was isolated from the root of Bupleurum talcatum L. and had a steroid-like structure, on murine thymocytes, and compared the action with that on spleen cells. Constitutive DNA synthesis or the growth response stimulated with anti...
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| Veröffentlicht in: | Immunopharmacology 1995-04, Vol.29 (3), p.207-213 |
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| creator | Kato, Masashi Pu, Mei-yi Isobe, Ken-Ichi Hattori, Taku Yanagita, Noriyuki Nakashima, Izumi |
| description | We examined the immunoregulatory action of saikosaponin-d (SSd), which was isolated from the root of Bupleurum talcatum L. and had a steroid-like structure, on murine thymocytes, and compared the action with that on spleen cells. Constitutive DNA synthesis or the growth response stimulated with anti-CD3mAb of thymocytes were down-regulated by 3 μg/ml SSd, whereas with spleen cells these were up-regulated by the same concentration of SSd. On the other hand, 3 μg/ml of SSd greatly up-regulated the growth response and interleukin 2 (IL-2)/interleukin 4 (IL-4) production induced through a receptor-bypassed pathway by calcium ionophore A23187 plus phorbol 12-myristate 13-acetate (PMA) in thymocytes, whereas it only slightly up-regulated them in spleen cells. Moreover, the same concentration of SSd inhibited DNA fragmentation in thymocytes induced by A23187 or PMA. These results suggest a unique cell type-dependent immuno-modulatory action of SSd. |
| doi_str_mv | 10.1016/0162-3109(95)00059-3 |
| format | Article |
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Constitutive DNA synthesis or the growth response stimulated with anti-CD3mAb of thymocytes were down-regulated by 3 μg/ml SSd, whereas with spleen cells these were up-regulated by the same concentration of SSd. On the other hand, 3 μg/ml of SSd greatly up-regulated the growth response and interleukin 2 (IL-2)/interleukin 4 (IL-4) production induced through a receptor-bypassed pathway by calcium ionophore A23187 plus phorbol 12-myristate 13-acetate (PMA) in thymocytes, whereas it only slightly up-regulated them in spleen cells. Moreover, the same concentration of SSd inhibited DNA fragmentation in thymocytes induced by A23187 or PMA. These results suggest a unique cell type-dependent immuno-modulatory action of SSd.</description><identifier>ISSN: 0162-3109</identifier><identifier>DOI: 10.1016/0162-3109(95)00059-3</identifier><identifier>PMID: 7622352</identifier><identifier>CODEN: IMMUDP</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Antibodies, Monoclonal - pharmacology ; Biological and medical sciences ; Calcimycin - pharmacology ; Cell Division - drug effects ; Cells, Cultured ; DNA Damage - immunology ; Down-Regulation ; General pharmacology ; Immuno-regulation ; Interleukin-2 - biosynthesis ; Interleukin-2 - genetics ; Interleukin-4 - biosynthesis ; Interleukin-4 - genetics ; Lymphocyte Activation - drug effects ; Lymphocyte Activation - genetics ; Lymphocyte Activation - immunology ; Medical sciences ; Mice ; Mice, Inbred C57BL ; Oleanolic Acid - analogs & derivatives ; Pharmacognosy. Homeopathy. Health food ; Pharmacology. Drug treatments ; Receptor-bypassed pathway ; Receptor-mediated pathway ; Receptors, Immunologic - drug effects ; Receptors, Immunologic - genetics ; Receptors, Immunologic - immunology ; Saikosaponin-d ; Sapogenins - pharmacology ; Saponins ; Signal Transduction - drug effects ; Signal Transduction - genetics ; Signal Transduction - immunology ; Spleen - cytology ; Spleen - drug effects ; Spleen - immunology ; T-Lymphocytes - drug effects ; T-Lymphocytes - immunology ; Thymus Gland - cytology ; Up-Regulation</subject><ispartof>Immunopharmacology, 1995-04, Vol.29 (3), p.207-213</ispartof><rights>1995 Elsevier Science B.V. All rights reserved</rights><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c452t-580feb1dbda1d33702e4a6688d8d6a54c227ce5ab5945157b9e1e292144017263</citedby><cites>FETCH-LOGICAL-c452t-580feb1dbda1d33702e4a6688d8d6a54c227ce5ab5945157b9e1e292144017263</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3479210$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7622352$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kato, Masashi</creatorcontrib><creatorcontrib>Pu, Mei-yi</creatorcontrib><creatorcontrib>Isobe, Ken-Ichi</creatorcontrib><creatorcontrib>Hattori, Taku</creatorcontrib><creatorcontrib>Yanagita, Noriyuki</creatorcontrib><creatorcontrib>Nakashima, Izumi</creatorcontrib><title>Cell type-oriented differential modulatory actions of saikosaponin-d on growth responses and DNA fragmentation of lymphocytes triggered by receptor-mediated and receptor-bypassed pathways</title><title>Immunopharmacology</title><addtitle>Immunopharmacology</addtitle><description>We examined the immunoregulatory action of saikosaponin-d (SSd), which was isolated from the root of Bupleurum talcatum L. and had a steroid-like structure, on murine thymocytes, and compared the action with that on spleen cells. Constitutive DNA synthesis or the growth response stimulated with anti-CD3mAb of thymocytes were down-regulated by 3 μg/ml SSd, whereas with spleen cells these were up-regulated by the same concentration of SSd. On the other hand, 3 μg/ml of SSd greatly up-regulated the growth response and interleukin 2 (IL-2)/interleukin 4 (IL-4) production induced through a receptor-bypassed pathway by calcium ionophore A23187 plus phorbol 12-myristate 13-acetate (PMA) in thymocytes, whereas it only slightly up-regulated them in spleen cells. Moreover, the same concentration of SSd inhibited DNA fragmentation in thymocytes induced by A23187 or PMA. These results suggest a unique cell type-dependent immuno-modulatory action of SSd.</description><subject>Animals</subject><subject>Antibodies, Monoclonal - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Calcimycin - pharmacology</subject><subject>Cell Division - drug effects</subject><subject>Cells, Cultured</subject><subject>DNA Damage - immunology</subject><subject>Down-Regulation</subject><subject>General pharmacology</subject><subject>Immuno-regulation</subject><subject>Interleukin-2 - biosynthesis</subject><subject>Interleukin-2 - genetics</subject><subject>Interleukin-4 - biosynthesis</subject><subject>Interleukin-4 - genetics</subject><subject>Lymphocyte Activation - drug effects</subject><subject>Lymphocyte Activation - genetics</subject><subject>Lymphocyte Activation - immunology</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Oleanolic Acid - analogs & derivatives</subject><subject>Pharmacognosy. Homeopathy. Health food</subject><subject>Pharmacology. Drug treatments</subject><subject>Receptor-bypassed pathway</subject><subject>Receptor-mediated pathway</subject><subject>Receptors, Immunologic - drug effects</subject><subject>Receptors, Immunologic - genetics</subject><subject>Receptors, Immunologic - immunology</subject><subject>Saikosaponin-d</subject><subject>Sapogenins - pharmacology</subject><subject>Saponins</subject><subject>Signal Transduction - drug effects</subject><subject>Signal Transduction - genetics</subject><subject>Signal Transduction - immunology</subject><subject>Spleen - cytology</subject><subject>Spleen - drug effects</subject><subject>Spleen - immunology</subject><subject>T-Lymphocytes - drug effects</subject><subject>T-Lymphocytes - immunology</subject><subject>Thymus Gland - cytology</subject><subject>Up-Regulation</subject><issn>0162-3109</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UU1v1DAQzQFUSss_AMkHhOgh1HbifFyQqoUCUlUucLYmtrNrSOLg8bbKb-ufY8Ku9sjBsjXz3pvxe1n2WvAPgovqmo7MC8Hb96264pyrNi-eZeen8ovsJeIvapR1q86ys7qSslDyPHvauGFgaZldHqJ3U3KWWd_3LtLbw8DGYPcDpBAXBib5MCELPUPwvwPCHCY_5ZaFiW1jeEw7Fh1SER0ymCz7dH_D-gjbkcRgJa_cYRnnXTBLIlCKfrulWZZ1C3GNm2lSPjrrYd1k1ThVu2UGRKrOkHaPsOBl9ryHAd2r432R_bz9_GPzNb_7_uXb5uYuN6WSKVcN710nbGdB2KKouXQlVFXT2MZWoEojZW2cgk61pRKq7lonnGylKEsualkVF9m7g-4cw5-9w6RHj4Z8g8mFPeq6LkVVqIaA5QFoYkCMrtdz9CPERQuu15z0GoheA9Gt0v9y0gXR3hz19x19_UQ6hkT9t8c-oIGBDJ2MxxOsoEyl4AT7eIA58uLBu6jRUKKGzCQPk7bB_3-Pv0g8tbA</recordid><startdate>19950401</startdate><enddate>19950401</enddate><creator>Kato, Masashi</creator><creator>Pu, Mei-yi</creator><creator>Isobe, Ken-Ichi</creator><creator>Hattori, Taku</creator><creator>Yanagita, Noriyuki</creator><creator>Nakashima, Izumi</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19950401</creationdate><title>Cell type-oriented differential modulatory actions of saikosaponin-d on growth responses and DNA fragmentation of lymphocytes triggered by receptor-mediated and receptor-bypassed pathways</title><author>Kato, Masashi ; Pu, Mei-yi ; Isobe, Ken-Ichi ; Hattori, Taku ; Yanagita, Noriyuki ; Nakashima, Izumi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c452t-580feb1dbda1d33702e4a6688d8d6a54c227ce5ab5945157b9e1e292144017263</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Calcimycin - pharmacology</topic><topic>Cell Division - drug effects</topic><topic>Cells, Cultured</topic><topic>DNA Damage - immunology</topic><topic>Down-Regulation</topic><topic>General pharmacology</topic><topic>Immuno-regulation</topic><topic>Interleukin-2 - biosynthesis</topic><topic>Interleukin-2 - genetics</topic><topic>Interleukin-4 - biosynthesis</topic><topic>Interleukin-4 - genetics</topic><topic>Lymphocyte Activation - drug effects</topic><topic>Lymphocyte Activation - genetics</topic><topic>Lymphocyte Activation - immunology</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Oleanolic Acid - analogs & derivatives</topic><topic>Pharmacognosy. Homeopathy. Health food</topic><topic>Pharmacology. Drug treatments</topic><topic>Receptor-bypassed pathway</topic><topic>Receptor-mediated pathway</topic><topic>Receptors, Immunologic - drug effects</topic><topic>Receptors, Immunologic - genetics</topic><topic>Receptors, Immunologic - immunology</topic><topic>Saikosaponin-d</topic><topic>Sapogenins - pharmacology</topic><topic>Saponins</topic><topic>Signal Transduction - drug effects</topic><topic>Signal Transduction - genetics</topic><topic>Signal Transduction - immunology</topic><topic>Spleen - cytology</topic><topic>Spleen - drug effects</topic><topic>Spleen - immunology</topic><topic>T-Lymphocytes - drug effects</topic><topic>T-Lymphocytes - immunology</topic><topic>Thymus Gland - cytology</topic><topic>Up-Regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kato, Masashi</creatorcontrib><creatorcontrib>Pu, Mei-yi</creatorcontrib><creatorcontrib>Isobe, Ken-Ichi</creatorcontrib><creatorcontrib>Hattori, Taku</creatorcontrib><creatorcontrib>Yanagita, Noriyuki</creatorcontrib><creatorcontrib>Nakashima, Izumi</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Immunopharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kato, Masashi</au><au>Pu, Mei-yi</au><au>Isobe, Ken-Ichi</au><au>Hattori, Taku</au><au>Yanagita, Noriyuki</au><au>Nakashima, Izumi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cell type-oriented differential modulatory actions of saikosaponin-d on growth responses and DNA fragmentation of lymphocytes triggered by receptor-mediated and receptor-bypassed pathways</atitle><jtitle>Immunopharmacology</jtitle><addtitle>Immunopharmacology</addtitle><date>1995-04-01</date><risdate>1995</risdate><volume>29</volume><issue>3</issue><spage>207</spage><epage>213</epage><pages>207-213</pages><issn>0162-3109</issn><coden>IMMUDP</coden><abstract>We examined the immunoregulatory action of saikosaponin-d (SSd), which was isolated from the root of Bupleurum talcatum L. and had a steroid-like structure, on murine thymocytes, and compared the action with that on spleen cells. Constitutive DNA synthesis or the growth response stimulated with anti-CD3mAb of thymocytes were down-regulated by 3 μg/ml SSd, whereas with spleen cells these were up-regulated by the same concentration of SSd. On the other hand, 3 μg/ml of SSd greatly up-regulated the growth response and interleukin 2 (IL-2)/interleukin 4 (IL-4) production induced through a receptor-bypassed pathway by calcium ionophore A23187 plus phorbol 12-myristate 13-acetate (PMA) in thymocytes, whereas it only slightly up-regulated them in spleen cells. Moreover, the same concentration of SSd inhibited DNA fragmentation in thymocytes induced by A23187 or PMA. These results suggest a unique cell type-dependent immuno-modulatory action of SSd.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>7622352</pmid><doi>10.1016/0162-3109(95)00059-3</doi><tpages>7</tpages></addata></record> |
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| ispartof | Immunopharmacology, 1995-04, Vol.29 (3), p.207-213 |
| issn | 0162-3109 |
| language | eng |
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| subjects | Animals Antibodies, Monoclonal - pharmacology Biological and medical sciences Calcimycin - pharmacology Cell Division - drug effects Cells, Cultured DNA Damage - immunology Down-Regulation General pharmacology Immuno-regulation Interleukin-2 - biosynthesis Interleukin-2 - genetics Interleukin-4 - biosynthesis Interleukin-4 - genetics Lymphocyte Activation - drug effects Lymphocyte Activation - genetics Lymphocyte Activation - immunology Medical sciences Mice Mice, Inbred C57BL Oleanolic Acid - analogs & derivatives Pharmacognosy. Homeopathy. Health food Pharmacology. Drug treatments Receptor-bypassed pathway Receptor-mediated pathway Receptors, Immunologic - drug effects Receptors, Immunologic - genetics Receptors, Immunologic - immunology Saikosaponin-d Sapogenins - pharmacology Saponins Signal Transduction - drug effects Signal Transduction - genetics Signal Transduction - immunology Spleen - cytology Spleen - drug effects Spleen - immunology T-Lymphocytes - drug effects T-Lymphocytes - immunology Thymus Gland - cytology Up-Regulation |
| title | Cell type-oriented differential modulatory actions of saikosaponin-d on growth responses and DNA fragmentation of lymphocytes triggered by receptor-mediated and receptor-bypassed pathways |
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