Detection and Immunolocalization of Human Erythrocyte Spectrin Immunoanalogues in Toxoplasma gondii (Protozoan, Parasite)
We demonstrated here the presence of proteins antigenically related to human erythroid spectrin in the parasitic protozoan Toxoplasma gondii. A high molecular weight doublet (M, 245‐240,000), present in equimolar ratio, and low molecular weight polypeptides (M, 75,000) were reacted with monoclonal a...
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| Veröffentlicht in: | The Journal of eukaryotic microbiology 1995-07, Vol.42 (4), p.427-433 |
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| creator | GHAZALI, MACHHOUR RODIER, MARIE-HELENE EL MOUDNI, BRAHIM BABIN, PHILIPPE FERNANDEZ, BEATRICE JACQUEMIN, JEAN-LOUIS |
| description | We demonstrated here the presence of proteins antigenically related to human erythroid spectrin in the parasitic protozoan Toxoplasma gondii. A high molecular weight doublet (M, 245‐240,000), present in equimolar ratio, and low molecular weight polypeptides (M, 75,000) were reacted with monoclonal and polyclonal anti‐human erythroid spectrin antibodies on electroblotted nitrocellulose sheets. Indirect immunofluorescence assay clearly showed that these proteins were localized in the anterior pole of the organism. Immunogold staining further revealed specific labeling of conoid, rhoptries, micronemes, and dense granules of the apical complex. The presence of the M, 245–240,000 doublet and the M, 75,000 spectrin‐like proteins in the anterior pole of T. gondii may probably be consistent with a structural stabilizer function in its organciles which are suspected to be involved in the process of host cell invasion. |
| doi_str_mv | 10.1111/j.1550-7408.1995.tb01607.x |
| format | Article |
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A high molecular weight doublet (M, 245‐240,000), present in equimolar ratio, and low molecular weight polypeptides (M, 75,000) were reacted with monoclonal and polyclonal anti‐human erythroid spectrin antibodies on electroblotted nitrocellulose sheets. Indirect immunofluorescence assay clearly showed that these proteins were localized in the anterior pole of the organism. Immunogold staining further revealed specific labeling of conoid, rhoptries, micronemes, and dense granules of the apical complex. The presence of the M, 245–240,000 doublet and the M, 75,000 spectrin‐like proteins in the anterior pole of T. gondii may probably be consistent with a structural stabilizer function in its organciles which are suspected to be involved in the process of host cell invasion.</description><identifier>ISSN: 1066-5234</identifier><identifier>EISSN: 1550-7408</identifier><identifier>DOI: 10.1111/j.1550-7408.1995.tb01607.x</identifier><identifier>PMID: 7620469</identifier><identifier>CODEN: JEMIED</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Animals ; Antibodies ; Antibodies, Monoclonal ; Apicomplexa ; Biological and medical sciences ; Cytoplasmic Granules - ultrastructure ; cytoskeleton ; Erythrocytes ; Fluorescent Antibody Technique ; Fundamental and applied biological sciences. Psychology ; Humans ; invasion ; Life cycle. Host-agent relationship. Pathogenesis ; Microscopy, Immunoelectron ; Molecular Weight ; Protozoa ; Protozoan Proteins - analysis ; spectrin ; Spectrin - analysis ; Toxoplasma - ultrastructure ; Toxoplasma gondii ; unicellular eukaryotes ; zoite</subject><ispartof>The Journal of eukaryotic microbiology, 1995-07, Vol.42 (4), p.427-433</ispartof><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3827-fd30cb05b3fb9689ee8400d9d393050c30cd1d495fdeca05d5bd3c501a307d8c3</citedby><cites>FETCH-LOGICAL-c3827-fd30cb05b3fb9689ee8400d9d393050c30cd1d495fdeca05d5bd3c501a307d8c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1550-7408.1995.tb01607.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1550-7408.1995.tb01607.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3630204$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7620469$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>GHAZALI, MACHHOUR</creatorcontrib><creatorcontrib>RODIER, MARIE-HELENE</creatorcontrib><creatorcontrib>EL MOUDNI, BRAHIM</creatorcontrib><creatorcontrib>BABIN, PHILIPPE</creatorcontrib><creatorcontrib>FERNANDEZ, BEATRICE</creatorcontrib><creatorcontrib>JACQUEMIN, JEAN-LOUIS</creatorcontrib><title>Detection and Immunolocalization of Human Erythrocyte Spectrin Immunoanalogues in Toxoplasma gondii (Protozoan, Parasite)</title><title>The Journal of eukaryotic microbiology</title><addtitle>J Eukaryot Microbiol</addtitle><description>We demonstrated here the presence of proteins antigenically related to human erythroid spectrin in the parasitic protozoan Toxoplasma gondii. A high molecular weight doublet (M, 245‐240,000), present in equimolar ratio, and low molecular weight polypeptides (M, 75,000) were reacted with monoclonal and polyclonal anti‐human erythroid spectrin antibodies on electroblotted nitrocellulose sheets. Indirect immunofluorescence assay clearly showed that these proteins were localized in the anterior pole of the organism. Immunogold staining further revealed specific labeling of conoid, rhoptries, micronemes, and dense granules of the apical complex. The presence of the M, 245–240,000 doublet and the M, 75,000 spectrin‐like proteins in the anterior pole of T. gondii may probably be consistent with a structural stabilizer function in its organciles which are suspected to be involved in the process of host cell invasion.</description><subject>Animals</subject><subject>Antibodies</subject><subject>Antibodies, Monoclonal</subject><subject>Apicomplexa</subject><subject>Biological and medical sciences</subject><subject>Cytoplasmic Granules - ultrastructure</subject><subject>cytoskeleton</subject><subject>Erythrocytes</subject><subject>Fluorescent Antibody Technique</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>invasion</subject><subject>Life cycle. Host-agent relationship. Pathogenesis</subject><subject>Microscopy, Immunoelectron</subject><subject>Molecular Weight</subject><subject>Protozoa</subject><subject>Protozoan Proteins - analysis</subject><subject>spectrin</subject><subject>Spectrin - analysis</subject><subject>Toxoplasma - ultrastructure</subject><subject>Toxoplasma gondii</subject><subject>unicellular eukaryotes</subject><subject>zoite</subject><issn>1066-5234</issn><issn>1550-7408</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkd1u1DAQhSNEVUrhEZAihFCRmjCOYzvhAqlatj-ogpVo4dJybKd4m8SLnYhNn75ON9pbhG9szfnOzFgnit4iSFE4H9cpIgQSlkORorIkaV8BosDS7bPoaC89D2-gNCEZzl9EL71fQ6AyhA6jQ0YzyGl5FI1fdK9lb2wXi07FV207dLaxUjTmQTyVbR1fDq3o4qUb-9_OyrHX8Y9NMDnTzQbRicbeDdrHoXRjt3bTCN-K-M52ypj4ZOVsbx8CdhqvhBPe9PrDq-igFo3Xr-f7OLo9X94sLpPr7xdXi7PrROIiY0mtMMgKSIXrqqRFqXWRA6hS4RIDARlUhVReklppKYAoUiksCSCBgalC4uPo_a7vxtk_YcWet8ZL3TSi03bwnLEcBRT9E0S0yDKKWQA_7UDprPdO13zjTCvcyBHwKSC-5lMKfEqBTwHxOSC-DeY385SharXaW-dEgv5u1oUPKdROdNL4PYYphgAG7PMO-2saPf7HAvzr8jbPpk8kuwbG93q7byDcPacMM8J_fbvg-PzniixoxjP8CCdfvkA</recordid><startdate>199507</startdate><enddate>199507</enddate><creator>GHAZALI, MACHHOUR</creator><creator>RODIER, MARIE-HELENE</creator><creator>EL MOUDNI, BRAHIM</creator><creator>BABIN, PHILIPPE</creator><creator>FERNANDEZ, BEATRICE</creator><creator>JACQUEMIN, JEAN-LOUIS</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>199507</creationdate><title>Detection and Immunolocalization of Human Erythrocyte Spectrin Immunoanalogues in Toxoplasma gondii (Protozoan, Parasite)</title><author>GHAZALI, MACHHOUR ; RODIER, MARIE-HELENE ; EL MOUDNI, BRAHIM ; BABIN, PHILIPPE ; FERNANDEZ, BEATRICE ; JACQUEMIN, JEAN-LOUIS</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3827-fd30cb05b3fb9689ee8400d9d393050c30cd1d495fdeca05d5bd3c501a307d8c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Animals</topic><topic>Antibodies</topic><topic>Antibodies, Monoclonal</topic><topic>Apicomplexa</topic><topic>Biological and medical sciences</topic><topic>Cytoplasmic Granules - ultrastructure</topic><topic>cytoskeleton</topic><topic>Erythrocytes</topic><topic>Fluorescent Antibody Technique</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>invasion</topic><topic>Life cycle. Host-agent relationship. Pathogenesis</topic><topic>Microscopy, Immunoelectron</topic><topic>Molecular Weight</topic><topic>Protozoa</topic><topic>Protozoan Proteins - analysis</topic><topic>spectrin</topic><topic>Spectrin - analysis</topic><topic>Toxoplasma - ultrastructure</topic><topic>Toxoplasma gondii</topic><topic>unicellular eukaryotes</topic><topic>zoite</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>GHAZALI, MACHHOUR</creatorcontrib><creatorcontrib>RODIER, MARIE-HELENE</creatorcontrib><creatorcontrib>EL MOUDNI, BRAHIM</creatorcontrib><creatorcontrib>BABIN, PHILIPPE</creatorcontrib><creatorcontrib>FERNANDEZ, BEATRICE</creatorcontrib><creatorcontrib>JACQUEMIN, JEAN-LOUIS</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of eukaryotic microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>GHAZALI, MACHHOUR</au><au>RODIER, MARIE-HELENE</au><au>EL MOUDNI, BRAHIM</au><au>BABIN, PHILIPPE</au><au>FERNANDEZ, BEATRICE</au><au>JACQUEMIN, JEAN-LOUIS</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection and Immunolocalization of Human Erythrocyte Spectrin Immunoanalogues in Toxoplasma gondii (Protozoan, Parasite)</atitle><jtitle>The Journal of eukaryotic microbiology</jtitle><addtitle>J Eukaryot Microbiol</addtitle><date>1995-07</date><risdate>1995</risdate><volume>42</volume><issue>4</issue><spage>427</spage><epage>433</epage><pages>427-433</pages><issn>1066-5234</issn><eissn>1550-7408</eissn><coden>JEMIED</coden><abstract>We demonstrated here the presence of proteins antigenically related to human erythroid spectrin in the parasitic protozoan Toxoplasma gondii. A high molecular weight doublet (M, 245‐240,000), present in equimolar ratio, and low molecular weight polypeptides (M, 75,000) were reacted with monoclonal and polyclonal anti‐human erythroid spectrin antibodies on electroblotted nitrocellulose sheets. Indirect immunofluorescence assay clearly showed that these proteins were localized in the anterior pole of the organism. Immunogold staining further revealed specific labeling of conoid, rhoptries, micronemes, and dense granules of the apical complex. The presence of the M, 245–240,000 doublet and the M, 75,000 spectrin‐like proteins in the anterior pole of T. gondii may probably be consistent with a structural stabilizer function in its organciles which are suspected to be involved in the process of host cell invasion.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>7620469</pmid><doi>10.1111/j.1550-7408.1995.tb01607.x</doi><tpages>7</tpages></addata></record> |
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| ispartof | The Journal of eukaryotic microbiology, 1995-07, Vol.42 (4), p.427-433 |
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| subjects | Animals Antibodies Antibodies, Monoclonal Apicomplexa Biological and medical sciences Cytoplasmic Granules - ultrastructure cytoskeleton Erythrocytes Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Humans invasion Life cycle. Host-agent relationship. Pathogenesis Microscopy, Immunoelectron Molecular Weight Protozoa Protozoan Proteins - analysis spectrin Spectrin - analysis Toxoplasma - ultrastructure Toxoplasma gondii unicellular eukaryotes zoite |
| title | Detection and Immunolocalization of Human Erythrocyte Spectrin Immunoanalogues in Toxoplasma gondii (Protozoan, Parasite) |
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