EGFR gene amplification ‐ rearrangement in human glioblastomas
Immunostaining using an affinity‐purified rabbit polyclonal antibody against the extracellular domain of the epidermal‐growth‐factor receptor (EGFR) showed over‐expression occurring in a fraction of tumor cells in 17 out of 18 human glioblastomas and in a majority of cells in 7 of the 18. Southern‐b...
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| Veröffentlicht in: | International journal of cancer 1995-07, Vol.62 (2), p.145-148 |
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| container_title | International journal of cancer |
| container_volume | 62 |
| creator | Schwechheimer, Karl Huang, Su Cavenee, Webster K. |
| description | Immunostaining using an affinity‐purified rabbit polyclonal antibody against the extracellular domain of the epidermal‐growth‐factor receptor (EGFR) showed over‐expression occurring in a fraction of tumor cells in 17 out of 18 human glioblastomas and in a majority of cells in 7 of the 18. Southern‐blotting technique using a full‐length EGFR cDNA probe showed a variable degree of amplification in 10 of the 17 glioblastomas, which was associated with EGFR over‐expression in each case. In 2 of the glioblastomas with EGFR gene amplification, a rearrangement of the gene affecting the extracellular domain of the receptor was identified and DNA sequence analyses revealed an identical deletion‐rearrangement of 801 base pairs between exons 2 to 7, resulting in an in‐frame fusion of exons I and 8. © 1995 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/ijc.2910620206 |
| format | Article |
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Southern‐blotting technique using a full‐length EGFR cDNA probe showed a variable degree of amplification in 10 of the 17 glioblastomas, which was associated with EGFR over‐expression in each case. In 2 of the glioblastomas with EGFR gene amplification, a rearrangement of the gene affecting the extracellular domain of the receptor was identified and DNA sequence analyses revealed an identical deletion‐rearrangement of 801 base pairs between exons 2 to 7, resulting in an in‐frame fusion of exons I and 8. © 1995 Wiley‐Liss, Inc.</description><identifier>ISSN: 0020-7136</identifier><identifier>EISSN: 1097-0215</identifier><identifier>DOI: 10.1002/ijc.2910620206</identifier><identifier>PMID: 7622287</identifier><identifier>CODEN: IJCNAW</identifier><language>eng</language><publisher>New York: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Base Sequence ; Biological and medical sciences ; Brain Neoplasms - genetics ; Cell Line ; DNA Primers - chemistry ; DNA, Neoplasm - genetics ; ErbB Receptors - genetics ; Gene Amplification ; Gene Expression Regulation, Neoplastic ; Gene Rearrangement ; Glioblastoma - genetics ; Humans ; Medical sciences ; Molecular Sequence Data ; Neurology ; RNA Splicing ; RNA, Neoplasm - genetics ; Tumors of the nervous system. 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Southern‐blotting technique using a full‐length EGFR cDNA probe showed a variable degree of amplification in 10 of the 17 glioblastomas, which was associated with EGFR over‐expression in each case. In 2 of the glioblastomas with EGFR gene amplification, a rearrangement of the gene affecting the extracellular domain of the receptor was identified and DNA sequence analyses revealed an identical deletion‐rearrangement of 801 base pairs between exons 2 to 7, resulting in an in‐frame fusion of exons I and 8. © 1995 Wiley‐Liss, Inc.</description><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Brain Neoplasms - genetics</subject><subject>Cell Line</subject><subject>DNA Primers - chemistry</subject><subject>DNA, Neoplasm - genetics</subject><subject>ErbB Receptors - genetics</subject><subject>Gene Amplification</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Gene Rearrangement</subject><subject>Glioblastoma - genetics</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Molecular Sequence Data</subject><subject>Neurology</subject><subject>RNA Splicing</subject><subject>RNA, Neoplasm - genetics</subject><subject>Tumors of the nervous system. Phacomatoses</subject><issn>0020-7136</issn><issn>1097-0215</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkD1PwzAQhi0EKqWwsiFlQGwpPiexkw1UtaWoEhKCObKdS3GVj2InQt34CfxGfgmpGhU2phve5947PYRcAh0DpezWrPWYJUA5o4zyIzIEmgifMoiOybADqC8g4KfkzLk1pQARDQdkIDhjLBZDcjedz569FVboyXJTmNxo2Zi68r4_vzyL0lpZrbDEqvFM5b21pay8VWFqVUjX1KV05-Qkl4XDi36OyOts-jJ58JdP88XkfunrMIi4HyvALGRRHOlYQAaQZyJmACIPGBNSYSYUMC5RhRHPcwx5rLiiGrNYBdh1jMjNvndj6_cWXZOWxmksCllh3bpUiBBYlAQdON6D2tbOWczTjTWltNsUaLpTlnbK0l9l3cJV39yqErMD3jvq8us-l07LIu-MaOMOWNDVcLa7m-yxD1Pg9p-j6eJx8ueFH9nahI8</recordid><startdate>19950717</startdate><enddate>19950717</enddate><creator>Schwechheimer, Karl</creator><creator>Huang, Su</creator><creator>Cavenee, Webster K.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19950717</creationdate><title>EGFR gene amplification ‐ rearrangement in human glioblastomas</title><author>Schwechheimer, Karl ; Huang, Su ; Cavenee, Webster K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4356-8b1ed42585c871d11fd782117f3227abed7b126aeb456ffe468b6b0ced8b3ec43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Brain Neoplasms - genetics</topic><topic>Cell Line</topic><topic>DNA Primers - chemistry</topic><topic>DNA, Neoplasm - genetics</topic><topic>ErbB Receptors - genetics</topic><topic>Gene Amplification</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Gene Rearrangement</topic><topic>Glioblastoma - genetics</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Molecular Sequence Data</topic><topic>Neurology</topic><topic>RNA Splicing</topic><topic>RNA, Neoplasm - genetics</topic><topic>Tumors of the nervous system. Phacomatoses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schwechheimer, Karl</creatorcontrib><creatorcontrib>Huang, Su</creatorcontrib><creatorcontrib>Cavenee, Webster K.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of cancer</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schwechheimer, Karl</au><au>Huang, Su</au><au>Cavenee, Webster K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>EGFR gene amplification ‐ rearrangement in human glioblastomas</atitle><jtitle>International journal of cancer</jtitle><addtitle>Int J Cancer</addtitle><date>1995-07-17</date><risdate>1995</risdate><volume>62</volume><issue>2</issue><spage>145</spage><epage>148</epage><pages>145-148</pages><issn>0020-7136</issn><eissn>1097-0215</eissn><coden>IJCNAW</coden><abstract>Immunostaining using an affinity‐purified rabbit polyclonal antibody against the extracellular domain of the epidermal‐growth‐factor receptor (EGFR) showed over‐expression occurring in a fraction of tumor cells in 17 out of 18 human glioblastomas and in a majority of cells in 7 of the 18. Southern‐blotting technique using a full‐length EGFR cDNA probe showed a variable degree of amplification in 10 of the 17 glioblastomas, which was associated with EGFR over‐expression in each case. In 2 of the glioblastomas with EGFR gene amplification, a rearrangement of the gene affecting the extracellular domain of the receptor was identified and DNA sequence analyses revealed an identical deletion‐rearrangement of 801 base pairs between exons 2 to 7, resulting in an in‐frame fusion of exons I and 8. © 1995 Wiley‐Liss, Inc.</abstract><cop>New York</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>7622287</pmid><doi>10.1002/ijc.2910620206</doi><tpages>4</tpages></addata></record> |
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| ispartof | International journal of cancer, 1995-07, Vol.62 (2), p.145-148 |
| issn | 0020-7136 1097-0215 |
| language | eng |
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| source | Wiley Online Library - AutoHoldings Journals; MEDLINE |
| subjects | Base Sequence Biological and medical sciences Brain Neoplasms - genetics Cell Line DNA Primers - chemistry DNA, Neoplasm - genetics ErbB Receptors - genetics Gene Amplification Gene Expression Regulation, Neoplastic Gene Rearrangement Glioblastoma - genetics Humans Medical sciences Molecular Sequence Data Neurology RNA Splicing RNA, Neoplasm - genetics Tumors of the nervous system. Phacomatoses |
| title | EGFR gene amplification ‐ rearrangement in human glioblastomas |
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