The head involution defective gene of Drosophila melanogaster functions in programmed cell death
Deletions of chromosomal region, 75C1,2 block virtually all programmed cell death (PCD) in the Drosophila embryo. We have identified a gene previously in this interval, reaper (rpr), which encodes an important regulator of PCD. Here we report the isolation of a second gene in this region, head invol...
Gespeichert in:
| Veröffentlicht in: | Genes & development 1995-07, Vol.9 (14), p.1694-1708 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1708 |
|---|---|
| container_issue | 14 |
| container_start_page | 1694 |
| container_title | Genes & development |
| container_volume | 9 |
| creator | Grether, M E Abrams, J M Agapite, J White, K Steller, H |
| description | Deletions of chromosomal region, 75C1,2 block virtually all programmed cell death (PCD) in the Drosophila embryo. We have identified a gene previously in this interval, reaper (rpr), which encodes an important regulator of PCD. Here we report the isolation of a second gene in this region, head involution defective (hid), which plays a similar role in PCD. hid mutant embryos have decreased levels of cell death and contain extra cells in the head. We have cloned the hid gene and find that its expression is sufficient to induce PCD in cell death defective mutants. The hid gene appears to encode a novel 410-amino-acid protein, and its mRNA is expressed in regions of the embryo where cell death occurs. Ectopic expression of hid in the Drosophila retina results in eye ablation. This phenotype can be suppressed completely by expression of the anti-apoptotic p35 protein from baculovirus, indicating that p35 may act genetically downstream from hid. |
| doi_str_mv | 10.1101/gad.9.14.1694 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_77410064</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>16882540</sourcerecordid><originalsourceid>FETCH-LOGICAL-c424t-934aa7a7bf53d74eda086341c2a35e759e82ac1979879d644e3412494fcf4a743</originalsourceid><addsrcrecordid>eNqFkD1PwzAQhi0EKqUwMiJ5Ykuwk4sdj6h8SpVYymyuyeUD5aPECRL_HketWJluuOdevfcwdi1FKKWQdyXmoQklhFIZOGFLmYAJEtD6lC1FakRgYmXO2YVzn0IIJZRasIVWUSRiWLKPbUW8Isx53X33zTTWfcdzKigb62_iJXXE-4I_DL3r91XdIG-pwa4v0Y008GLqsvnE-XO-H_pywLalnGfUND4Gx-qSnRXYOLo6zhV7f3rcrl-Czdvz6_p-E2QQwehLAqJGvSuSONdAOYpUxSCzCOOEdGIojTCTRptUm1wBkF9GYKDICkAN8YrdHnJ9i6-J3Gjb2s01sKN-clZrkP79_0Gp0jRKQHgwOICZf94NVNj9ULc4_Fgp7KzeevXWWAl2Vu_5m2PwtPMO_uij6_gXZYp_qQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16882540</pqid></control><display><type>article</type><title>The head involution defective gene of Drosophila melanogaster functions in programmed cell death</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>Grether, M E ; Abrams, J M ; Agapite, J ; White, K ; Steller, H</creator><creatorcontrib>Grether, M E ; Abrams, J M ; Agapite, J ; White, K ; Steller, H</creatorcontrib><description>Deletions of chromosomal region, 75C1,2 block virtually all programmed cell death (PCD) in the Drosophila embryo. We have identified a gene previously in this interval, reaper (rpr), which encodes an important regulator of PCD. Here we report the isolation of a second gene in this region, head involution defective (hid), which plays a similar role in PCD. hid mutant embryos have decreased levels of cell death and contain extra cells in the head. We have cloned the hid gene and find that its expression is sufficient to induce PCD in cell death defective mutants. The hid gene appears to encode a novel 410-amino-acid protein, and its mRNA is expressed in regions of the embryo where cell death occurs. Ectopic expression of hid in the Drosophila retina results in eye ablation. This phenotype can be suppressed completely by expression of the anti-apoptotic p35 protein from baculovirus, indicating that p35 may act genetically downstream from hid.</description><identifier>ISSN: 0890-9369</identifier><identifier>EISSN: 1549-5477</identifier><identifier>DOI: 10.1101/gad.9.14.1694</identifier><identifier>PMID: 7622034</identifier><language>eng</language><publisher>United States</publisher><subject>Alleles ; Amino Acid Sequence ; Animals ; Apoptosis - genetics ; Base Sequence ; Chromosome Mapping ; Cloning, Molecular ; DNA - genetics ; DNA Primers - genetics ; Drosophila melanogaster ; Drosophila melanogaster - cytology ; Drosophila melanogaster - embryology ; Drosophila melanogaster - genetics ; Drosophila Proteins ; Eye Abnormalities - embryology ; Eye Abnormalities - genetics ; Gene Deletion ; Genes, Insect ; Models, Genetic ; Molecular Sequence Data ; Neuropeptides - genetics ; RNA, Messenger - genetics</subject><ispartof>Genes & development, 1995-07, Vol.9 (14), p.1694-1708</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c424t-934aa7a7bf53d74eda086341c2a35e759e82ac1979879d644e3412494fcf4a743</citedby><cites>FETCH-LOGICAL-c424t-934aa7a7bf53d74eda086341c2a35e759e82ac1979879d644e3412494fcf4a743</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7622034$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Grether, M E</creatorcontrib><creatorcontrib>Abrams, J M</creatorcontrib><creatorcontrib>Agapite, J</creatorcontrib><creatorcontrib>White, K</creatorcontrib><creatorcontrib>Steller, H</creatorcontrib><title>The head involution defective gene of Drosophila melanogaster functions in programmed cell death</title><title>Genes & development</title><addtitle>Genes Dev</addtitle><description>Deletions of chromosomal region, 75C1,2 block virtually all programmed cell death (PCD) in the Drosophila embryo. We have identified a gene previously in this interval, reaper (rpr), which encodes an important regulator of PCD. Here we report the isolation of a second gene in this region, head involution defective (hid), which plays a similar role in PCD. hid mutant embryos have decreased levels of cell death and contain extra cells in the head. We have cloned the hid gene and find that its expression is sufficient to induce PCD in cell death defective mutants. The hid gene appears to encode a novel 410-amino-acid protein, and its mRNA is expressed in regions of the embryo where cell death occurs. Ectopic expression of hid in the Drosophila retina results in eye ablation. This phenotype can be suppressed completely by expression of the anti-apoptotic p35 protein from baculovirus, indicating that p35 may act genetically downstream from hid.</description><subject>Alleles</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Apoptosis - genetics</subject><subject>Base Sequence</subject><subject>Chromosome Mapping</subject><subject>Cloning, Molecular</subject><subject>DNA - genetics</subject><subject>DNA Primers - genetics</subject><subject>Drosophila melanogaster</subject><subject>Drosophila melanogaster - cytology</subject><subject>Drosophila melanogaster - embryology</subject><subject>Drosophila melanogaster - genetics</subject><subject>Drosophila Proteins</subject><subject>Eye Abnormalities - embryology</subject><subject>Eye Abnormalities - genetics</subject><subject>Gene Deletion</subject><subject>Genes, Insect</subject><subject>Models, Genetic</subject><subject>Molecular Sequence Data</subject><subject>Neuropeptides - genetics</subject><subject>RNA, Messenger - genetics</subject><issn>0890-9369</issn><issn>1549-5477</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkD1PwzAQhi0EKqUwMiJ5Ykuwk4sdj6h8SpVYymyuyeUD5aPECRL_HketWJluuOdevfcwdi1FKKWQdyXmoQklhFIZOGFLmYAJEtD6lC1FakRgYmXO2YVzn0IIJZRasIVWUSRiWLKPbUW8Isx53X33zTTWfcdzKigb62_iJXXE-4I_DL3r91XdIG-pwa4v0Y008GLqsvnE-XO-H_pywLalnGfUND4Gx-qSnRXYOLo6zhV7f3rcrl-Czdvz6_p-E2QQwehLAqJGvSuSONdAOYpUxSCzCOOEdGIojTCTRptUm1wBkF9GYKDICkAN8YrdHnJ9i6-J3Gjb2s01sKN-clZrkP79_0Gp0jRKQHgwOICZf94NVNj9ULc4_Fgp7KzeevXWWAl2Vu_5m2PwtPMO_uij6_gXZYp_qQ</recordid><startdate>19950715</startdate><enddate>19950715</enddate><creator>Grether, M E</creator><creator>Abrams, J M</creator><creator>Agapite, J</creator><creator>White, K</creator><creator>Steller, H</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19950715</creationdate><title>The head involution defective gene of Drosophila melanogaster functions in programmed cell death</title><author>Grether, M E ; Abrams, J M ; Agapite, J ; White, K ; Steller, H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c424t-934aa7a7bf53d74eda086341c2a35e759e82ac1979879d644e3412494fcf4a743</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Alleles</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Apoptosis - genetics</topic><topic>Base Sequence</topic><topic>Chromosome Mapping</topic><topic>Cloning, Molecular</topic><topic>DNA - genetics</topic><topic>DNA Primers - genetics</topic><topic>Drosophila melanogaster</topic><topic>Drosophila melanogaster - cytology</topic><topic>Drosophila melanogaster - embryology</topic><topic>Drosophila melanogaster - genetics</topic><topic>Drosophila Proteins</topic><topic>Eye Abnormalities - embryology</topic><topic>Eye Abnormalities - genetics</topic><topic>Gene Deletion</topic><topic>Genes, Insect</topic><topic>Models, Genetic</topic><topic>Molecular Sequence Data</topic><topic>Neuropeptides - genetics</topic><topic>RNA, Messenger - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Grether, M E</creatorcontrib><creatorcontrib>Abrams, J M</creatorcontrib><creatorcontrib>Agapite, J</creatorcontrib><creatorcontrib>White, K</creatorcontrib><creatorcontrib>Steller, H</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Genes & development</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Grether, M E</au><au>Abrams, J M</au><au>Agapite, J</au><au>White, K</au><au>Steller, H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The head involution defective gene of Drosophila melanogaster functions in programmed cell death</atitle><jtitle>Genes & development</jtitle><addtitle>Genes Dev</addtitle><date>1995-07-15</date><risdate>1995</risdate><volume>9</volume><issue>14</issue><spage>1694</spage><epage>1708</epage><pages>1694-1708</pages><issn>0890-9369</issn><eissn>1549-5477</eissn><abstract>Deletions of chromosomal region, 75C1,2 block virtually all programmed cell death (PCD) in the Drosophila embryo. We have identified a gene previously in this interval, reaper (rpr), which encodes an important regulator of PCD. Here we report the isolation of a second gene in this region, head involution defective (hid), which plays a similar role in PCD. hid mutant embryos have decreased levels of cell death and contain extra cells in the head. We have cloned the hid gene and find that its expression is sufficient to induce PCD in cell death defective mutants. The hid gene appears to encode a novel 410-amino-acid protein, and its mRNA is expressed in regions of the embryo where cell death occurs. Ectopic expression of hid in the Drosophila retina results in eye ablation. This phenotype can be suppressed completely by expression of the anti-apoptotic p35 protein from baculovirus, indicating that p35 may act genetically downstream from hid.</abstract><cop>United States</cop><pmid>7622034</pmid><doi>10.1101/gad.9.14.1694</doi><tpages>15</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0890-9369 |
| ispartof | Genes & development, 1995-07, Vol.9 (14), p.1694-1708 |
| issn | 0890-9369 1549-5477 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_77410064 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Alleles Amino Acid Sequence Animals Apoptosis - genetics Base Sequence Chromosome Mapping Cloning, Molecular DNA - genetics DNA Primers - genetics Drosophila melanogaster Drosophila melanogaster - cytology Drosophila melanogaster - embryology Drosophila melanogaster - genetics Drosophila Proteins Eye Abnormalities - embryology Eye Abnormalities - genetics Gene Deletion Genes, Insect Models, Genetic Molecular Sequence Data Neuropeptides - genetics RNA, Messenger - genetics |
| title | The head involution defective gene of Drosophila melanogaster functions in programmed cell death |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-09T10%3A18%3A00IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20head%20involution%20defective%20gene%20of%20Drosophila%20melanogaster%20functions%20in%20programmed%20cell%20death&rft.jtitle=Genes%20&%20development&rft.au=Grether,%20M%20E&rft.date=1995-07-15&rft.volume=9&rft.issue=14&rft.spage=1694&rft.epage=1708&rft.pages=1694-1708&rft.issn=0890-9369&rft.eissn=1549-5477&rft_id=info:doi/10.1101/gad.9.14.1694&rft_dat=%3Cproquest_cross%3E16882540%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=16882540&rft_id=info:pmid/7622034&rfr_iscdi=true |