Compartmentation of 14CO2 in the perfused rat liver
The specific activity of the mitochondrial CO2 + bicarbonate system has been measured in perfused livers using the specific activities of urea and acetoacetate derived from 2-ketoisocaproate catabolism. Label was supplied either as NaH14CO3, 2-keto[1-14C]isocaproate, [1-14C]pyruvate, [1-14C]glutamin...
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| Veröffentlicht in: | The Journal of biological chemistry 1987-02, Vol.262 (6), p.2604-2607 |
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| creator | Marsolais, C. Huot, S. David, F. Garneau, M. Brunengraber, H. |
| description | The specific activity of the mitochondrial CO2 + bicarbonate system has been measured in perfused livers using the specific activities of urea and acetoacetate derived from 2-ketoisocaproate catabolism. Label was supplied either as NaH14CO3, 2-keto[1-14C]isocaproate, [1-14C]pyruvate, [1-14C]glutamine, or [14C]formate. With labeled bicarbonate, pyruvate, or 2-ketoisocaproate, the specific activities of effluent bicarbonate, urea, and acetoacetate were equal (acetoacetate was labeled only on C-1). In the presence of [14C]formate, the specific activity of acetoacetate was double that of urea. Acetazolamide (0.2 mM), an inhibitor of carbonic anhydrase, decreased the specific activities of urea and acetoacetate labeled from NaH14CO3 and increased the specific activities of urea and acetoacetate labeled from the other tracers. We conclude that: acetoacetate derived from 2-ketoisocaproate is, like urea, an index of the specific activity of mitochondrial CO2 in liver, carbonic anhydrase activity equalizes the specific activities of the CO2 + bicarbonate system on both sides of the mitochondrial membrane, and a fraction of [14C] formate-derived 14CO2 appears to be generated in a mitochondrial compartment, in the close vicinity of methylcrotonyl-CoA carboxylase. |
| doi_str_mv | 10.1016/S0021-9258(18)61548-0 |
| format | Article |
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Label was supplied either as NaH14CO3, 2-keto[1-14C]isocaproate, [1-14C]pyruvate, [1-14C]glutamine, or [14C]formate. With labeled bicarbonate, pyruvate, or 2-ketoisocaproate, the specific activities of effluent bicarbonate, urea, and acetoacetate were equal (acetoacetate was labeled only on C-1). In the presence of [14C]formate, the specific activity of acetoacetate was double that of urea. Acetazolamide (0.2 mM), an inhibitor of carbonic anhydrase, decreased the specific activities of urea and acetoacetate labeled from NaH14CO3 and increased the specific activities of urea and acetoacetate labeled from the other tracers. We conclude that: acetoacetate derived from 2-ketoisocaproate is, like urea, an index of the specific activity of mitochondrial CO2 in liver, carbonic anhydrase activity equalizes the specific activities of the CO2 + bicarbonate system on both sides of the mitochondrial membrane, and a fraction of [14C] formate-derived 14CO2 appears to be generated in a mitochondrial compartment, in the close vicinity of methylcrotonyl-CoA carboxylase.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1016/S0021-9258(18)61548-0</identifier><identifier>PMID: 3102472</identifier><identifier>CODEN: JBCHA3</identifier><language>eng</language><publisher>Bethesda, MD: Elsevier Inc</publisher><subject>Acetazolamide - pharmacology ; Acetoacetates - metabolism ; Analytical biochemistry: general aspects, technics, instrumentation ; Analytical, structural and metabolic biochemistry ; Animals ; Biological and medical sciences ; Carbon Dioxide - metabolism ; Cell Compartmentation ; Formates - metabolism ; Fundamental and applied biological sciences. 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Label was supplied either as NaH14CO3, 2-keto[1-14C]isocaproate, [1-14C]pyruvate, [1-14C]glutamine, or [14C]formate. With labeled bicarbonate, pyruvate, or 2-ketoisocaproate, the specific activities of effluent bicarbonate, urea, and acetoacetate were equal (acetoacetate was labeled only on C-1). In the presence of [14C]formate, the specific activity of acetoacetate was double that of urea. Acetazolamide (0.2 mM), an inhibitor of carbonic anhydrase, decreased the specific activities of urea and acetoacetate labeled from NaH14CO3 and increased the specific activities of urea and acetoacetate labeled from the other tracers. We conclude that: acetoacetate derived from 2-ketoisocaproate is, like urea, an index of the specific activity of mitochondrial CO2 in liver, carbonic anhydrase activity equalizes the specific activities of the CO2 + bicarbonate system on both sides of the mitochondrial membrane, and a fraction of [14C] formate-derived 14CO2 appears to be generated in a mitochondrial compartment, in the close vicinity of methylcrotonyl-CoA carboxylase.</description><subject>Acetazolamide - pharmacology</subject><subject>Acetoacetates - metabolism</subject><subject>Analytical biochemistry: general aspects, technics, instrumentation</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Carbon Dioxide - metabolism</subject><subject>Cell Compartmentation</subject><subject>Formates - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glutamine - metabolism</subject><subject>Keto Acids - metabolism</subject><subject>Liver - cytology</subject><subject>Liver - metabolism</subject><subject>Pyruvates - metabolism</subject><subject>Pyruvic Acid</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Urea - metabolism</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1LJDEQhoOsuOPHTxAaWWT30JpKutPJaVmGdRUEDyp4C_modrL0x5j0KP57M84wV3NIDvW8qaqHkFOgF0BBXN5TyqBUrJY_Qf4SUFeypHtkBlTyktfw9I3Mdsh3cpjSf5pPpeCAHHCgrGrYjPD52C9NnHocJjOFcSjGtoBqfseKMBTTAoslxnaV0BfRTEUXXjEek_3WdAlPtu8Rebz6-zC_Lm_v_t3M_9yWjjeSll7UVvjaNkaiN5QCcInCWKWQCy4VtVwoVNyigIo1iiunWkDfCqucZZ4fkfPNv8s4vqwwTboPyWHXmQHHVdJNU9G8N81gvQFdHFOK2OplDL2J7xqoXsvSn7L02oQGqT9l6XXudNtgZXv0u9TWTq7_2NZNcqZroxlcSDtMguKibjJ2tsEW4XnxFiJqG0a3wF4zwbTIN60y9HsDYTb2GjDq5AIODn0OuEn7MXwx7QfIq48V</recordid><startdate>19870225</startdate><enddate>19870225</enddate><creator>Marsolais, C.</creator><creator>Huot, S.</creator><creator>David, F.</creator><creator>Garneau, M.</creator><creator>Brunengraber, H.</creator><general>Elsevier Inc</general><general>American Society for Biochemistry and Molecular Biology</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19870225</creationdate><title>Compartmentation of 14CO2 in the perfused rat liver</title><author>Marsolais, C. ; Huot, S. ; David, F. ; Garneau, M. ; Brunengraber, H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3780-d65b6d5b7a8eda001138e6ab99e363890b369e93be61427939c9f1edf6b9cb2d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Acetazolamide - pharmacology</topic><topic>Acetoacetates - metabolism</topic><topic>Analytical biochemistry: general aspects, technics, instrumentation</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Carbon Dioxide - metabolism</topic><topic>Cell Compartmentation</topic><topic>Formates - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glutamine - metabolism</topic><topic>Keto Acids - metabolism</topic><topic>Liver - cytology</topic><topic>Liver - metabolism</topic><topic>Pyruvates - metabolism</topic><topic>Pyruvic Acid</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Urea - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Marsolais, C.</creatorcontrib><creatorcontrib>Huot, S.</creatorcontrib><creatorcontrib>David, F.</creatorcontrib><creatorcontrib>Garneau, M.</creatorcontrib><creatorcontrib>Brunengraber, H.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Marsolais, C.</au><au>Huot, S.</au><au>David, F.</au><au>Garneau, M.</au><au>Brunengraber, H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Compartmentation of 14CO2 in the perfused rat liver</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1987-02-25</date><risdate>1987</risdate><volume>262</volume><issue>6</issue><spage>2604</spage><epage>2607</epage><pages>2604-2607</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><coden>JBCHA3</coden><abstract>The specific activity of the mitochondrial CO2 + bicarbonate system has been measured in perfused livers using the specific activities of urea and acetoacetate derived from 2-ketoisocaproate catabolism. Label was supplied either as NaH14CO3, 2-keto[1-14C]isocaproate, [1-14C]pyruvate, [1-14C]glutamine, or [14C]formate. With labeled bicarbonate, pyruvate, or 2-ketoisocaproate, the specific activities of effluent bicarbonate, urea, and acetoacetate were equal (acetoacetate was labeled only on C-1). In the presence of [14C]formate, the specific activity of acetoacetate was double that of urea. Acetazolamide (0.2 mM), an inhibitor of carbonic anhydrase, decreased the specific activities of urea and acetoacetate labeled from NaH14CO3 and increased the specific activities of urea and acetoacetate labeled from the other tracers. We conclude that: acetoacetate derived from 2-ketoisocaproate is, like urea, an index of the specific activity of mitochondrial CO2 in liver, carbonic anhydrase activity equalizes the specific activities of the CO2 + bicarbonate system on both sides of the mitochondrial membrane, and a fraction of [14C] formate-derived 14CO2 appears to be generated in a mitochondrial compartment, in the close vicinity of methylcrotonyl-CoA carboxylase.</abstract><cop>Bethesda, MD</cop><pub>Elsevier Inc</pub><pmid>3102472</pmid><doi>10.1016/S0021-9258(18)61548-0</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Acetazolamide - pharmacology Acetoacetates - metabolism Analytical biochemistry: general aspects, technics, instrumentation Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Carbon Dioxide - metabolism Cell Compartmentation Formates - metabolism Fundamental and applied biological sciences. Psychology Glutamine - metabolism Keto Acids - metabolism Liver - cytology Liver - metabolism Pyruvates - metabolism Pyruvic Acid Rats Rats, Inbred Strains Urea - metabolism |
| title | Compartmentation of 14CO2 in the perfused rat liver |
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