A Computer-Assisted Image-Analysis System for Analyzing Polymorphonuclear Leukocyte Chemotaxis in Patients with Diabetes Mellitus
A computer-assisted image-analysis system that precisely tracks the cell movements of up to 50 polymorphonuclear leukocytes (PMNLs) was developed and used to quantitatively measure cellular chemokinesis and chemotaxis in normal individuals and in diabetic patients with hyperglycemia. The PMNLs were...
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| Veröffentlicht in: | The Journal of infectious diseases 1987-04, Vol.155 (4), p.737-741 |
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| container_title | The Journal of infectious diseases |
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| creator | Donovan, Richard M. Goldstein, Elliot Kim, Yoo Lippert, William Kailath, Elizabeth Aoki, Thomas T. Cheung, Anthony T. W. Miller, Michael E. Chang, Daniel P. |
| description | A computer-assisted image-analysis system that precisely tracks the cell movements of up to 50 polymorphonuclear leukocytes (PMNLs) was developed and used to quantitatively measure cellular chemokinesis and chemotaxis in normal individuals and in diabetic patients with hyperglycemia. The PMNLs were tested in Zigmond chambers with or without a gradient of 10-7 M n-formylmethionylleucylphenylalanine (f-Met-Leu-Phe), Cellular movement was recorded on videotape by using a videocamera mounted on the microscope. The videotapes were analyzed by computer programs to calculate the speed and direction of each PMNL at to-sec intervals. Average rates of chemokinesis were 19.6 μm/min without and 25.3 μm/min with f-Met-Leu-Phe. McCutcheon indices, which measure chemotaxis, were 0.01 without and 0.48 with f-Met-Leu-Phe, Similar values were observed in diabetic patients after fasting (average glucose, 217 mg/1OO ml) and 2 hr after glucose challenge (average glucose, 309 mg/1OO ml), These values demonstrate that PMNLs from diabetic patients with hyperglycemia move at normal rates and respond appropriately to f-Met-Leu-Phe. |
| doi_str_mv | 10.1093/infdis/155.4.737 |
| format | Article |
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W. ; Miller, Michael E. ; Chang, Daniel P.</creator><creatorcontrib>Donovan, Richard M. ; Goldstein, Elliot ; Kim, Yoo ; Lippert, William ; Kailath, Elizabeth ; Aoki, Thomas T. ; Cheung, Anthony T. W. ; Miller, Michael E. ; Chang, Daniel P.</creatorcontrib><description>A computer-assisted image-analysis system that precisely tracks the cell movements of up to 50 polymorphonuclear leukocytes (PMNLs) was developed and used to quantitatively measure cellular chemokinesis and chemotaxis in normal individuals and in diabetic patients with hyperglycemia. The PMNLs were tested in Zigmond chambers with or without a gradient of 10-7 M n-formylmethionylleucylphenylalanine (f-Met-Leu-Phe), Cellular movement was recorded on videotape by using a videocamera mounted on the microscope. The videotapes were analyzed by computer programs to calculate the speed and direction of each PMNL at to-sec intervals. Average rates of chemokinesis were 19.6 μm/min without and 25.3 μm/min with f-Met-Leu-Phe. McCutcheon indices, which measure chemotaxis, were 0.01 without and 0.48 with f-Met-Leu-Phe, Similar values were observed in diabetic patients after fasting (average glucose, 217 mg/1OO ml) and 2 hr after glucose challenge (average glucose, 309 mg/1OO ml), These values demonstrate that PMNLs from diabetic patients with hyperglycemia move at normal rates and respond appropriately to f-Met-Leu-Phe.</description><identifier>ISSN: 0022-1899</identifier><identifier>EISSN: 1537-6613</identifier><identifier>DOI: 10.1093/infdis/155.4.737</identifier><identifier>PMID: 3819478</identifier><identifier>CODEN: JIDIAQ</identifier><language>eng</language><publisher>Chicago, IL: The University of Chicago Press</publisher><subject>Adolescent ; Adult ; Aged ; Biological and medical sciences ; Blood cells ; Cell motility ; Chemotactic factors ; Chemotaxis ; Chemotaxis, Leukocyte ; Diabetes ; Diabetes complications ; Diabetes mellitus ; Diabetes Mellitus - blood ; Endocrine pancreas. Apud cells (diseases) ; Endocrinopathies ; Female ; Humans ; Hyperglycemia - blood ; Image Processing, Computer-Assisted ; Leukocytes ; Locomotion ; Male ; Medical sciences ; Middle Aged ; N-Formylmethionine Leucyl-Phenylalanine ; Neutrophils ; Neutrophils - physiology ; Original Articles ; Tumors. Hypoglycemia ; Videotape Recording</subject><ispartof>The Journal of infectious diseases, 1987-04, Vol.155 (4), p.737-741</ispartof><rights>Copyright 1987 The University of Chicago</rights><rights>1988 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c414t-ee8c716bcda06f3c937d9faab4abda516f5fd20b35177d60f7a8c3766cde4f493</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/30105843$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/30105843$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,27923,27924,58016,58249</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7619614$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3819478$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Donovan, Richard M.</creatorcontrib><creatorcontrib>Goldstein, Elliot</creatorcontrib><creatorcontrib>Kim, Yoo</creatorcontrib><creatorcontrib>Lippert, William</creatorcontrib><creatorcontrib>Kailath, Elizabeth</creatorcontrib><creatorcontrib>Aoki, Thomas T.</creatorcontrib><creatorcontrib>Cheung, Anthony T. W.</creatorcontrib><creatorcontrib>Miller, Michael E.</creatorcontrib><creatorcontrib>Chang, Daniel P.</creatorcontrib><title>A Computer-Assisted Image-Analysis System for Analyzing Polymorphonuclear Leukocyte Chemotaxis in Patients with Diabetes Mellitus</title><title>The Journal of infectious diseases</title><addtitle>J Infect Dis</addtitle><description>A computer-assisted image-analysis system that precisely tracks the cell movements of up to 50 polymorphonuclear leukocytes (PMNLs) was developed and used to quantitatively measure cellular chemokinesis and chemotaxis in normal individuals and in diabetic patients with hyperglycemia. The PMNLs were tested in Zigmond chambers with or without a gradient of 10-7 M n-formylmethionylleucylphenylalanine (f-Met-Leu-Phe), Cellular movement was recorded on videotape by using a videocamera mounted on the microscope. The videotapes were analyzed by computer programs to calculate the speed and direction of each PMNL at to-sec intervals. Average rates of chemokinesis were 19.6 μm/min without and 25.3 μm/min with f-Met-Leu-Phe. McCutcheon indices, which measure chemotaxis, were 0.01 without and 0.48 with f-Met-Leu-Phe, Similar values were observed in diabetic patients after fasting (average glucose, 217 mg/1OO ml) and 2 hr after glucose challenge (average glucose, 309 mg/1OO ml), These values demonstrate that PMNLs from diabetic patients with hyperglycemia move at normal rates and respond appropriately to f-Met-Leu-Phe.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Biological and medical sciences</subject><subject>Blood cells</subject><subject>Cell motility</subject><subject>Chemotactic factors</subject><subject>Chemotaxis</subject><subject>Chemotaxis, Leukocyte</subject><subject>Diabetes</subject><subject>Diabetes complications</subject><subject>Diabetes mellitus</subject><subject>Diabetes Mellitus - blood</subject><subject>Endocrine pancreas. Apud cells (diseases)</subject><subject>Endocrinopathies</subject><subject>Female</subject><subject>Humans</subject><subject>Hyperglycemia - blood</subject><subject>Image Processing, Computer-Assisted</subject><subject>Leukocytes</subject><subject>Locomotion</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>N-Formylmethionine Leucyl-Phenylalanine</subject><subject>Neutrophils</subject><subject>Neutrophils - physiology</subject><subject>Original Articles</subject><subject>Tumors. Hypoglycemia</subject><subject>Videotape Recording</subject><issn>0022-1899</issn><issn>1537-6613</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkbtvEzEcxy0EKqGwsyB5QGyX2vHrPEYptIUgyktCLJbPZzdu787B9okeG_85DgntyGTp-9LP-gDwHKM5RpKc-MG1Pp1gxuZ0Loh4AGaYEVFxjslDMENosahwLeVj8CSla4QQJVwcgSNSY0lFPQO_l3AV-u2YbayWKfmUbQsven1lq-Wgu6ko8PNU1B66EOFf7ZcfruBl6KY-xO0mDKPprI5wbcebYKZs4Wpj-5D1ben6AV7q7O2QE_zp8waeet3YbBN8b7vO5zE9BY-c7pJ9dniPwdc3r7-szqv1h7OL1XJdGYpprqytjcC8Ma1G3BEjiWil07qhumk1w9wx1y5QQxgWouXICV0bIjg3raWOSnIMXu13tzH8GG3KqvfJlCP0YMOYlBAUMcbIf4OYCoK45CWI9kETQ0rROrWNvtdxUhipHR61x6MKHkVVwVMqLw7bY9Pb9q5w4FH8lwdfJ6M7F_VgysC_mOBYckzvZ65TDvF-BWHEarr7QrX3dzxv73wdbxQvZzB1_u27km8_fRRnC6HekT_rhbWd</recordid><startdate>19870401</startdate><enddate>19870401</enddate><creator>Donovan, Richard M.</creator><creator>Goldstein, Elliot</creator><creator>Kim, Yoo</creator><creator>Lippert, William</creator><creator>Kailath, Elizabeth</creator><creator>Aoki, Thomas T.</creator><creator>Cheung, Anthony T. W.</creator><creator>Miller, Michael E.</creator><creator>Chang, Daniel P.</creator><general>The University of Chicago Press</general><general>University of Chicago Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7T5</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19870401</creationdate><title>A Computer-Assisted Image-Analysis System for Analyzing Polymorphonuclear Leukocyte Chemotaxis in Patients with Diabetes Mellitus</title><author>Donovan, Richard M. ; Goldstein, Elliot ; Kim, Yoo ; Lippert, William ; Kailath, Elizabeth ; Aoki, Thomas T. ; Cheung, Anthony T. W. ; Miller, Michael E. ; Chang, Daniel P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c414t-ee8c716bcda06f3c937d9faab4abda516f5fd20b35177d60f7a8c3766cde4f493</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Biological and medical sciences</topic><topic>Blood cells</topic><topic>Cell motility</topic><topic>Chemotactic factors</topic><topic>Chemotaxis</topic><topic>Chemotaxis, Leukocyte</topic><topic>Diabetes</topic><topic>Diabetes complications</topic><topic>Diabetes mellitus</topic><topic>Diabetes Mellitus - blood</topic><topic>Endocrine pancreas. Apud cells (diseases)</topic><topic>Endocrinopathies</topic><topic>Female</topic><topic>Humans</topic><topic>Hyperglycemia - blood</topic><topic>Image Processing, Computer-Assisted</topic><topic>Leukocytes</topic><topic>Locomotion</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>N-Formylmethionine Leucyl-Phenylalanine</topic><topic>Neutrophils</topic><topic>Neutrophils - physiology</topic><topic>Original Articles</topic><topic>Tumors. Hypoglycemia</topic><topic>Videotape Recording</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Donovan, Richard M.</creatorcontrib><creatorcontrib>Goldstein, Elliot</creatorcontrib><creatorcontrib>Kim, Yoo</creatorcontrib><creatorcontrib>Lippert, William</creatorcontrib><creatorcontrib>Kailath, Elizabeth</creatorcontrib><creatorcontrib>Aoki, Thomas T.</creatorcontrib><creatorcontrib>Cheung, Anthony T. W.</creatorcontrib><creatorcontrib>Miller, Michael E.</creatorcontrib><creatorcontrib>Chang, Daniel P.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Donovan, Richard M.</au><au>Goldstein, Elliot</au><au>Kim, Yoo</au><au>Lippert, William</au><au>Kailath, Elizabeth</au><au>Aoki, Thomas T.</au><au>Cheung, Anthony T. W.</au><au>Miller, Michael E.</au><au>Chang, Daniel P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Computer-Assisted Image-Analysis System for Analyzing Polymorphonuclear Leukocyte Chemotaxis in Patients with Diabetes Mellitus</atitle><jtitle>The Journal of infectious diseases</jtitle><addtitle>J Infect Dis</addtitle><date>1987-04-01</date><risdate>1987</risdate><volume>155</volume><issue>4</issue><spage>737</spage><epage>741</epage><pages>737-741</pages><issn>0022-1899</issn><eissn>1537-6613</eissn><coden>JIDIAQ</coden><abstract>A computer-assisted image-analysis system that precisely tracks the cell movements of up to 50 polymorphonuclear leukocytes (PMNLs) was developed and used to quantitatively measure cellular chemokinesis and chemotaxis in normal individuals and in diabetic patients with hyperglycemia. The PMNLs were tested in Zigmond chambers with or without a gradient of 10-7 M n-formylmethionylleucylphenylalanine (f-Met-Leu-Phe), Cellular movement was recorded on videotape by using a videocamera mounted on the microscope. The videotapes were analyzed by computer programs to calculate the speed and direction of each PMNL at to-sec intervals. Average rates of chemokinesis were 19.6 μm/min without and 25.3 μm/min with f-Met-Leu-Phe. McCutcheon indices, which measure chemotaxis, were 0.01 without and 0.48 with f-Met-Leu-Phe, Similar values were observed in diabetic patients after fasting (average glucose, 217 mg/1OO ml) and 2 hr after glucose challenge (average glucose, 309 mg/1OO ml), These values demonstrate that PMNLs from diabetic patients with hyperglycemia move at normal rates and respond appropriately to f-Met-Leu-Phe.</abstract><cop>Chicago, IL</cop><pub>The University of Chicago Press</pub><pmid>3819478</pmid><doi>10.1093/infdis/155.4.737</doi><tpages>5</tpages></addata></record> |
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| ispartof | The Journal of infectious diseases, 1987-04, Vol.155 (4), p.737-741 |
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| source | MEDLINE; JSTOR Archive Collection A-Z Listing |
| subjects | Adolescent Adult Aged Biological and medical sciences Blood cells Cell motility Chemotactic factors Chemotaxis Chemotaxis, Leukocyte Diabetes Diabetes complications Diabetes mellitus Diabetes Mellitus - blood Endocrine pancreas. Apud cells (diseases) Endocrinopathies Female Humans Hyperglycemia - blood Image Processing, Computer-Assisted Leukocytes Locomotion Male Medical sciences Middle Aged N-Formylmethionine Leucyl-Phenylalanine Neutrophils Neutrophils - physiology Original Articles Tumors. Hypoglycemia Videotape Recording |
| title | A Computer-Assisted Image-Analysis System for Analyzing Polymorphonuclear Leukocyte Chemotaxis in Patients with Diabetes Mellitus |
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