Bathophenanthrolene Disulfonic Acid and Sodium Dithionite Effectively Remove Surface-Bound Iron from Caco-2 Cell Monolayers

Iron uptake by Caco-2 cell monolayers is commonly assessed by incubating the cells under radiolabeled iron solutions, removing the radiolabeled solution, rinsing to stop uptake and measuring the radioactivity retained by the cells. It is therefore essential to differentiate between iron that is nons...

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Veröffentlicht in:	The Journal of nutrition 1995-07, Vol.125 (7), p.1833-1840
Hauptverfasser:	Glahn, Raymond P., Gangloff, Mary Beth, van Campen, Darrell R., Miller, Dennis D., Wien, Elizabeth M., Norvell, Wendell A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Biochemistry Biological and medical sciences Biological Transport - physiology Caco-2 Cell Membrane - chemistry Cell Membrane - metabolism Cell Membrane - ultrastructure Cellular biology Colon - chemistry Colon - drug effects Colon - metabolism Colonic Neoplasms - chemistry Colonic Neoplasms - metabolism Colonic Neoplasms - ultrastructure Dithionite - pharmacology Epithelium - chemistry Epithelium - drug effects Epithelium - metabolism ferritin Fundamental and applied biological sciences. Psychology Humans Intestine. Mesentery Iron Iron - analysis Iron - metabolism iron removal iron uptake Phenanthrolines - pharmacology Tumor Cells, Cultured Vertebrates: digestive system
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