Induction of heat-shock protein 72 protects against ischemia/reperfusion in rat small intestine
Background & Aims: Induction of heat-shock protein 72 is associated with enhanced tolerance to subsequent nonthermal stresses. This study evaluated whether induction of heat-shock protein 72 protects against intestinal ischemia/reperfusion injury. Methods: Groups of nonheated and heated rats und...
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| Veröffentlicht in: | Gastroenterology (New York, N.Y. 1943) N.Y. 1943), 1995-08, Vol.109 (2), p.505-515 |
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| container_title | Gastroenterology (New York, N.Y. 1943) |
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| creator | Stojadinovic, Alexander Kiang, Juliann Smallridge, Robert Galloway, Richard Shea-Donohue, Terez |
| description | Background & Aims:
Induction of heat-shock protein 72 is associated with enhanced tolerance to subsequent nonthermal stresses. This study evaluated whether induction of heat-shock protein 72 protects against intestinal ischemia/reperfusion injury.
Methods:
Groups of nonheated and heated rats underwent sham operation, 30 minutes of ischemia by occlusion of the superior mesenteric artery, or ischemia followed by 60 minutes of reperfusion. Whole-body hyperthermia to a core temperature of 41.5–42°C for 15–20 minutes was followed by passive cooling 2–3 hours before the experiment. Samples of small intestine were obtained for determination of heat-shock protein 72 production and ex vivo generation of prostaglandin E
2 and leukotriene B
4 and for histological assessment of mucosal injury and number of neutrophils.
Results:
Hyperthermia significantly increased heat-shock protein 72 production and significantly reduced ischemia/reperfusion-induced mucosal injury, neutrophilic infiltration, and leukotriene B
4 production. Levels of leukotriene B
4 and numbers of neutrophils were well correlated in nonheated (
r = 0.72) but not in heated groups (
r = −0.16). The elevation of prostaglandin E
2 levels in response to ischemia and reperfusion was unaltered by hyperthermia.
Conclusions:
The mechanism of heat stress-induced protection against intestinal ischemia/reperfusion injury involves inhibition of leukotriene B
4 production and subsequent prevention of neutrophil activation and chemotaxis. |
| doi_str_mv | 10.1016/0016-5085(95)90339-9 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_77402710</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0016508595903399</els_id><sourcerecordid>77402710</sourcerecordid><originalsourceid>FETCH-LOGICAL-c452t-afee4454b60d4f7d2586ba045f76f9c2a0d61221501ad365627cd8e8e177f2313</originalsourceid><addsrcrecordid>eNp9kF1LHDEUhoNU7Gr9By3MhYhejJ5k8jFzI4i0VRC8aa9DNjnpRudjTTJC_30z7LKXhZAPznNOXh5CvlK4oUDlLZStFtCKq05cd9A0Xd0dkRUVrK1LjX0iqwPymZym9AoAXdPSE3KiZMEAVkQ_jW62OUxjNflqgybXaTPZt2obp4xhrBTbXW1OlfljwphyFZLd4BDMbcQtRj-npb2w0eQqDabvyyNjymHEL-TYmz7h-f48I79_fP_18Fg_v_x8erh_ri0XLNfGI3Iu-FqC4145Jlq5NsCFV9J3lhlwkjJGBVDjGikkU9a12CJVyrOGNmfkcje3hH2fy996KCmx782I05y0UhyYolBAvgNtnFKK6PU2hsHEv5qCXrzqRZpepOmurMWr7krbt_38eT2gOzTtRZb6xb5ukjW9j2a0IR2wRjLGYYl5t8OwuPgIGHWyAUeLLsSiWLsp_D_HP-8ek90</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>77402710</pqid></control><display><type>article</type><title>Induction of heat-shock protein 72 protects against ischemia/reperfusion in rat small intestine</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><source>Alma/SFX Local Collection</source><creator>Stojadinovic, Alexander ; Kiang, Juliann ; Smallridge, Robert ; Galloway, Richard ; Shea-Donohue, Terez</creator><creatorcontrib>Stojadinovic, Alexander ; Kiang, Juliann ; Smallridge, Robert ; Galloway, Richard ; Shea-Donohue, Terez</creatorcontrib><description>Background & Aims:
Induction of heat-shock protein 72 is associated with enhanced tolerance to subsequent nonthermal stresses. This study evaluated whether induction of heat-shock protein 72 protects against intestinal ischemia/reperfusion injury.
Methods:
Groups of nonheated and heated rats underwent sham operation, 30 minutes of ischemia by occlusion of the superior mesenteric artery, or ischemia followed by 60 minutes of reperfusion. Whole-body hyperthermia to a core temperature of 41.5–42°C for 15–20 minutes was followed by passive cooling 2–3 hours before the experiment. Samples of small intestine were obtained for determination of heat-shock protein 72 production and ex vivo generation of prostaglandin E
2 and leukotriene B
4 and for histological assessment of mucosal injury and number of neutrophils.
Results:
Hyperthermia significantly increased heat-shock protein 72 production and significantly reduced ischemia/reperfusion-induced mucosal injury, neutrophilic infiltration, and leukotriene B
4 production. Levels of leukotriene B
4 and numbers of neutrophils were well correlated in nonheated (
r = 0.72) but not in heated groups (
r = −0.16). The elevation of prostaglandin E
2 levels in response to ischemia and reperfusion was unaltered by hyperthermia.
Conclusions:
The mechanism of heat stress-induced protection against intestinal ischemia/reperfusion injury involves inhibition of leukotriene B
4 production and subsequent prevention of neutrophil activation and chemotaxis.</description><identifier>ISSN: 0016-5085</identifier><identifier>EISSN: 1528-0012</identifier><identifier>DOI: 10.1016/0016-5085(95)90339-9</identifier><identifier>PMID: 7615200</identifier><identifier>CODEN: GASTAB</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Animals ; Biological and medical sciences ; Blood Pressure - physiology ; Fever ; Gastroenterology. Liver. Pancreas. Abdomen ; Heart Rate - physiology ; Heat-Shock Proteins - biosynthesis ; HSP72 Heat-Shock Proteins ; Intestinal Mucosa - blood supply ; Intestinal Mucosa - metabolism ; Intestinal Mucosa - pathology ; Intestine, Small - blood supply ; Intestine, Small - metabolism ; Intestine, Small - pathology ; Leukotriene B4 - biosynthesis ; Male ; Medical sciences ; Neutrophils - physiology ; Other diseases. Semiology ; Rats ; Rats, Sprague-Dawley ; Reperfusion Injury - metabolism ; Reperfusion Injury - pathology ; Reperfusion Injury - prevention & control ; Stomach. Duodenum. Small intestine. Colon. Rectum. Anus</subject><ispartof>Gastroenterology (New York, N.Y. 1943), 1995-08, Vol.109 (2), p.505-515</ispartof><rights>1995</rights><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c452t-afee4454b60d4f7d2586ba045f76f9c2a0d61221501ad365627cd8e8e177f2313</citedby><cites>FETCH-LOGICAL-c452t-afee4454b60d4f7d2586ba045f76f9c2a0d61221501ad365627cd8e8e177f2313</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0016508595903399$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3622401$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7615200$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Stojadinovic, Alexander</creatorcontrib><creatorcontrib>Kiang, Juliann</creatorcontrib><creatorcontrib>Smallridge, Robert</creatorcontrib><creatorcontrib>Galloway, Richard</creatorcontrib><creatorcontrib>Shea-Donohue, Terez</creatorcontrib><title>Induction of heat-shock protein 72 protects against ischemia/reperfusion in rat small intestine</title><title>Gastroenterology (New York, N.Y. 1943)</title><addtitle>Gastroenterology</addtitle><description>Background & Aims:
Induction of heat-shock protein 72 is associated with enhanced tolerance to subsequent nonthermal stresses. This study evaluated whether induction of heat-shock protein 72 protects against intestinal ischemia/reperfusion injury.
Methods:
Groups of nonheated and heated rats underwent sham operation, 30 minutes of ischemia by occlusion of the superior mesenteric artery, or ischemia followed by 60 minutes of reperfusion. Whole-body hyperthermia to a core temperature of 41.5–42°C for 15–20 minutes was followed by passive cooling 2–3 hours before the experiment. Samples of small intestine were obtained for determination of heat-shock protein 72 production and ex vivo generation of prostaglandin E
2 and leukotriene B
4 and for histological assessment of mucosal injury and number of neutrophils.
Results:
Hyperthermia significantly increased heat-shock protein 72 production and significantly reduced ischemia/reperfusion-induced mucosal injury, neutrophilic infiltration, and leukotriene B
4 production. Levels of leukotriene B
4 and numbers of neutrophils were well correlated in nonheated (
r = 0.72) but not in heated groups (
r = −0.16). The elevation of prostaglandin E
2 levels in response to ischemia and reperfusion was unaltered by hyperthermia.
Conclusions:
The mechanism of heat stress-induced protection against intestinal ischemia/reperfusion injury involves inhibition of leukotriene B
4 production and subsequent prevention of neutrophil activation and chemotaxis.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blood Pressure - physiology</subject><subject>Fever</subject><subject>Gastroenterology. Liver. Pancreas. Abdomen</subject><subject>Heart Rate - physiology</subject><subject>Heat-Shock Proteins - biosynthesis</subject><subject>HSP72 Heat-Shock Proteins</subject><subject>Intestinal Mucosa - blood supply</subject><subject>Intestinal Mucosa - metabolism</subject><subject>Intestinal Mucosa - pathology</subject><subject>Intestine, Small - blood supply</subject><subject>Intestine, Small - metabolism</subject><subject>Intestine, Small - pathology</subject><subject>Leukotriene B4 - biosynthesis</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Neutrophils - physiology</subject><subject>Other diseases. Semiology</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Reperfusion Injury - metabolism</subject><subject>Reperfusion Injury - pathology</subject><subject>Reperfusion Injury - prevention & control</subject><subject>Stomach. Duodenum. Small intestine. Colon. Rectum. Anus</subject><issn>0016-5085</issn><issn>1528-0012</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kF1LHDEUhoNU7Gr9By3MhYhejJ5k8jFzI4i0VRC8aa9DNjnpRudjTTJC_30z7LKXhZAPznNOXh5CvlK4oUDlLZStFtCKq05cd9A0Xd0dkRUVrK1LjX0iqwPymZym9AoAXdPSE3KiZMEAVkQ_jW62OUxjNflqgybXaTPZt2obp4xhrBTbXW1OlfljwphyFZLd4BDMbcQtRj-npb2w0eQqDabvyyNjymHEL-TYmz7h-f48I79_fP_18Fg_v_x8erh_ri0XLNfGI3Iu-FqC4145Jlq5NsCFV9J3lhlwkjJGBVDjGikkU9a12CJVyrOGNmfkcje3hH2fy996KCmx782I05y0UhyYolBAvgNtnFKK6PU2hsHEv5qCXrzqRZpepOmurMWr7krbt_38eT2gOzTtRZb6xb5ukjW9j2a0IR2wRjLGYYl5t8OwuPgIGHWyAUeLLsSiWLsp_D_HP-8ek90</recordid><startdate>19950801</startdate><enddate>19950801</enddate><creator>Stojadinovic, Alexander</creator><creator>Kiang, Juliann</creator><creator>Smallridge, Robert</creator><creator>Galloway, Richard</creator><creator>Shea-Donohue, Terez</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19950801</creationdate><title>Induction of heat-shock protein 72 protects against ischemia/reperfusion in rat small intestine</title><author>Stojadinovic, Alexander ; Kiang, Juliann ; Smallridge, Robert ; Galloway, Richard ; Shea-Donohue, Terez</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c452t-afee4454b60d4f7d2586ba045f76f9c2a0d61221501ad365627cd8e8e177f2313</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blood Pressure - physiology</topic><topic>Fever</topic><topic>Gastroenterology. Liver. Pancreas. Abdomen</topic><topic>Heart Rate - physiology</topic><topic>Heat-Shock Proteins - biosynthesis</topic><topic>HSP72 Heat-Shock Proteins</topic><topic>Intestinal Mucosa - blood supply</topic><topic>Intestinal Mucosa - metabolism</topic><topic>Intestinal Mucosa - pathology</topic><topic>Intestine, Small - blood supply</topic><topic>Intestine, Small - metabolism</topic><topic>Intestine, Small - pathology</topic><topic>Leukotriene B4 - biosynthesis</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Neutrophils - physiology</topic><topic>Other diseases. Semiology</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Reperfusion Injury - metabolism</topic><topic>Reperfusion Injury - pathology</topic><topic>Reperfusion Injury - prevention & control</topic><topic>Stomach. Duodenum. Small intestine. Colon. Rectum. Anus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Stojadinovic, Alexander</creatorcontrib><creatorcontrib>Kiang, Juliann</creatorcontrib><creatorcontrib>Smallridge, Robert</creatorcontrib><creatorcontrib>Galloway, Richard</creatorcontrib><creatorcontrib>Shea-Donohue, Terez</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Gastroenterology (New York, N.Y. 1943)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Stojadinovic, Alexander</au><au>Kiang, Juliann</au><au>Smallridge, Robert</au><au>Galloway, Richard</au><au>Shea-Donohue, Terez</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Induction of heat-shock protein 72 protects against ischemia/reperfusion in rat small intestine</atitle><jtitle>Gastroenterology (New York, N.Y. 1943)</jtitle><addtitle>Gastroenterology</addtitle><date>1995-08-01</date><risdate>1995</risdate><volume>109</volume><issue>2</issue><spage>505</spage><epage>515</epage><pages>505-515</pages><issn>0016-5085</issn><eissn>1528-0012</eissn><coden>GASTAB</coden><abstract>Background & Aims:
Induction of heat-shock protein 72 is associated with enhanced tolerance to subsequent nonthermal stresses. This study evaluated whether induction of heat-shock protein 72 protects against intestinal ischemia/reperfusion injury.
Methods:
Groups of nonheated and heated rats underwent sham operation, 30 minutes of ischemia by occlusion of the superior mesenteric artery, or ischemia followed by 60 minutes of reperfusion. Whole-body hyperthermia to a core temperature of 41.5–42°C for 15–20 minutes was followed by passive cooling 2–3 hours before the experiment. Samples of small intestine were obtained for determination of heat-shock protein 72 production and ex vivo generation of prostaglandin E
2 and leukotriene B
4 and for histological assessment of mucosal injury and number of neutrophils.
Results:
Hyperthermia significantly increased heat-shock protein 72 production and significantly reduced ischemia/reperfusion-induced mucosal injury, neutrophilic infiltration, and leukotriene B
4 production. Levels of leukotriene B
4 and numbers of neutrophils were well correlated in nonheated (
r = 0.72) but not in heated groups (
r = −0.16). The elevation of prostaglandin E
2 levels in response to ischemia and reperfusion was unaltered by hyperthermia.
Conclusions:
The mechanism of heat stress-induced protection against intestinal ischemia/reperfusion injury involves inhibition of leukotriene B
4 production and subsequent prevention of neutrophil activation and chemotaxis.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>7615200</pmid><doi>10.1016/0016-5085(95)90339-9</doi><tpages>11</tpages></addata></record> |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals; Alma/SFX Local Collection |
| subjects | Animals Biological and medical sciences Blood Pressure - physiology Fever Gastroenterology. Liver. Pancreas. Abdomen Heart Rate - physiology Heat-Shock Proteins - biosynthesis HSP72 Heat-Shock Proteins Intestinal Mucosa - blood supply Intestinal Mucosa - metabolism Intestinal Mucosa - pathology Intestine, Small - blood supply Intestine, Small - metabolism Intestine, Small - pathology Leukotriene B4 - biosynthesis Male Medical sciences Neutrophils - physiology Other diseases. Semiology Rats Rats, Sprague-Dawley Reperfusion Injury - metabolism Reperfusion Injury - pathology Reperfusion Injury - prevention & control Stomach. Duodenum. Small intestine. Colon. Rectum. Anus |
| title | Induction of heat-shock protein 72 protects against ischemia/reperfusion in rat small intestine |
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