FIV infection of macrophages: in vitro and in vivo inhibition by dideoxycytidine 5′-triphosphate
We have evaluated in vitro and in vivo whether it is possible to protect cat macrophages from feline immunodeficiency virus (FIV) infection by the administration of dideoxycytidine 5′-triphosphate (DDCTP). Since cell membranes are impermeable to phosphorylated drugs we have encapsulated DDCTP into a...
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| Veröffentlicht in: | Veterinary immunology and immunopathology 1995-05, Vol.46 (1), p.151-158 |
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| container_title | Veterinary immunology and immunopathology |
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| creator | Magnani, M. Rossi, L. Fraternale, A. Silvotti, L. Quintavalla, F. Piedimonte, G. Matteucci, D. Baldinotti, F. Bendinelli, M. |
| description | We have evaluated in vitro and in vivo whether it is possible to protect cat macrophages from feline immunodeficiency virus (FIV) infection by the administration of dideoxycytidine 5′-triphosphate (DDCTP). Since cell membranes are impermeable to phosphorylated drugs we have encapsulated DDCTP into autologous erythrocytes and modified erythrocyte membranes to target these drug-loaded cells to macrophages. DDCTP-loaded erythrocytes reduced FIV production by macrophages infected in vitro or obtained from naturally or experimentally infected cats. The same treatment protected the majority of peritoneal macrophages during a 7 month experimental FIV infection and reduced the percentage of circulating lymphocytes stained with an anti-p24 antibody. These results suggest that the administration of nucleoside analogues in phosphorylated form is feasible and their targeting to macrophages reduces FIV infection in vitro and in vivo. |
| doi_str_mv | 10.1016/0165-2427(94)07014-X |
| format | Article |
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Since cell membranes are impermeable to phosphorylated drugs we have encapsulated DDCTP into autologous erythrocytes and modified erythrocyte membranes to target these drug-loaded cells to macrophages. DDCTP-loaded erythrocytes reduced FIV production by macrophages infected in vitro or obtained from naturally or experimentally infected cats. The same treatment protected the majority of peritoneal macrophages during a 7 month experimental FIV infection and reduced the percentage of circulating lymphocytes stained with an anti-p24 antibody. These results suggest that the administration of nucleoside analogues in phosphorylated form is feasible and their targeting to macrophages reduces FIV infection in vitro and in vivo.</description><identifier>ISSN: 0165-2427</identifier><identifier>EISSN: 1873-2534</identifier><identifier>DOI: 10.1016/0165-2427(94)07014-X</identifier><identifier>PMID: 7618255</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>AIDS/HIV ; ANIMAL VIRUSES ; Animals ; ANTIVIRAL ; ANTIVIRAL AGENTS ; Antiviral Agents - administration & dosage ; Antiviral Agents - pharmacology ; CATS ; CHAT ; DEFICIENCE IMMUNOLOGIQUE ; DEFICIENCIA INMUNOLOGICA ; Deoxycytosine Nucleotides - administration & dosage ; Deoxycytosine Nucleotides - pharmacology ; Dideoxynucleotides ; Drug Carriers ; ERITROCITOS ; ERYTHROCYTE ; ERYTHROCYTES ; Feline Acquired Immunodeficiency Syndrome - blood ; Feline Acquired Immunodeficiency Syndrome - drug therapy ; Feline Acquired Immunodeficiency Syndrome - immunology ; feline leukemia virus ; Female ; GATO ; Immunodeficiency Virus, Feline - drug effects ; Immunodeficiency Virus, Feline - physiology ; IMMUNOLOGICAL DEFICIENCY ; Lymphocytes - virology ; MACROFAGOS ; MACROPHAGE ; MACROPHAGES ; Macrophages - virology ; Macrophages, Peritoneal - virology ; Male ; Specific Pathogen-Free Organisms ; VIRICIDAS ; VIRUS DE LOS ANIMALES ; VIRUS DES ANIMAUX ; Virus Replication - drug effects</subject><ispartof>Veterinary immunology and immunopathology, 1995-05, Vol.46 (1), p.151-158</ispartof><rights>1995</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c322t-9fedb5a5a8e9855b4c0e93cd09a30748e6d55929fad269c2402b742ac78b212c3</citedby><cites>FETCH-LOGICAL-c322t-9fedb5a5a8e9855b4c0e93cd09a30748e6d55929fad269c2402b742ac78b212c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0165-2427(94)07014-X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7618255$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Magnani, M.</creatorcontrib><creatorcontrib>Rossi, L.</creatorcontrib><creatorcontrib>Fraternale, A.</creatorcontrib><creatorcontrib>Silvotti, L.</creatorcontrib><creatorcontrib>Quintavalla, F.</creatorcontrib><creatorcontrib>Piedimonte, G.</creatorcontrib><creatorcontrib>Matteucci, D.</creatorcontrib><creatorcontrib>Baldinotti, F.</creatorcontrib><creatorcontrib>Bendinelli, M.</creatorcontrib><title>FIV infection of macrophages: in vitro and in vivo inhibition by dideoxycytidine 5′-triphosphate</title><title>Veterinary immunology and immunopathology</title><addtitle>Vet Immunol Immunopathol</addtitle><description>We have evaluated in vitro and in vivo whether it is possible to protect cat macrophages from feline immunodeficiency virus (FIV) infection by the administration of dideoxycytidine 5′-triphosphate (DDCTP). Since cell membranes are impermeable to phosphorylated drugs we have encapsulated DDCTP into autologous erythrocytes and modified erythrocyte membranes to target these drug-loaded cells to macrophages. DDCTP-loaded erythrocytes reduced FIV production by macrophages infected in vitro or obtained from naturally or experimentally infected cats. The same treatment protected the majority of peritoneal macrophages during a 7 month experimental FIV infection and reduced the percentage of circulating lymphocytes stained with an anti-p24 antibody. These results suggest that the administration of nucleoside analogues in phosphorylated form is feasible and their targeting to macrophages reduces FIV infection in vitro and in vivo.</description><subject>AIDS/HIV</subject><subject>ANIMAL VIRUSES</subject><subject>Animals</subject><subject>ANTIVIRAL</subject><subject>ANTIVIRAL AGENTS</subject><subject>Antiviral Agents - administration & dosage</subject><subject>Antiviral Agents - pharmacology</subject><subject>CATS</subject><subject>CHAT</subject><subject>DEFICIENCE IMMUNOLOGIQUE</subject><subject>DEFICIENCIA INMUNOLOGICA</subject><subject>Deoxycytosine Nucleotides - administration & dosage</subject><subject>Deoxycytosine Nucleotides - pharmacology</subject><subject>Dideoxynucleotides</subject><subject>Drug Carriers</subject><subject>ERITROCITOS</subject><subject>ERYTHROCYTE</subject><subject>ERYTHROCYTES</subject><subject>Feline Acquired Immunodeficiency Syndrome - blood</subject><subject>Feline Acquired Immunodeficiency Syndrome - drug therapy</subject><subject>Feline Acquired Immunodeficiency Syndrome - immunology</subject><subject>feline leukemia virus</subject><subject>Female</subject><subject>GATO</subject><subject>Immunodeficiency Virus, Feline - drug effects</subject><subject>Immunodeficiency Virus, Feline - physiology</subject><subject>IMMUNOLOGICAL DEFICIENCY</subject><subject>Lymphocytes - virology</subject><subject>MACROFAGOS</subject><subject>MACROPHAGE</subject><subject>MACROPHAGES</subject><subject>Macrophages - virology</subject><subject>Macrophages, Peritoneal - virology</subject><subject>Male</subject><subject>Specific Pathogen-Free Organisms</subject><subject>VIRICIDAS</subject><subject>VIRUS DE LOS ANIMALES</subject><subject>VIRUS DES ANIMAUX</subject><subject>Virus Replication - drug effects</subject><issn>0165-2427</issn><issn>1873-2534</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1KJDEUhcMw4rSOLzDMQK1EF-UkqaSSuBBE_INmZqPiLqSSW3aku9Im1Y2985l8pHmSSVuNS12ESzjnngvfQegnwUcEk_p3frykjIoDxQ6xwISV91_QiEhRlZRX7CsavVu-oZ2UHjHGXEm5jbZFTSTlfISai-u7wnct2N6HrghtMTM2hvnEPEA6zkqx9H0Mhenc8FmGPCe-8W_-ZlU47yA8r-yq9853UPB_L69lH_18ElKO6eE72mrNNMHeZu6i24vzm7Orcvz38vrsdFzaitK-VC24hhtuJCjJecMsBlVZh5WpsGASase5oqo1jtbKUoZpIxg1VsiGEmqrXbQ_5M5jeFpA6vXMJwvTqekgLJIWgmFCGfvUSATGohI0G9lgzERSitDqefQzE1eaYL3uQK8B6zVgrZh-60Df57Vfm_xFMwP3vrSBnvUfg96aoM1D9En_GSuOmWR1Fk8GETKqpYeok_XQWXA-5pK0C_7j6_8BwhOfWA</recordid><startdate>199505</startdate><enddate>199505</enddate><creator>Magnani, M.</creator><creator>Rossi, L.</creator><creator>Fraternale, A.</creator><creator>Silvotti, L.</creator><creator>Quintavalla, F.</creator><creator>Piedimonte, G.</creator><creator>Matteucci, D.</creator><creator>Baldinotti, F.</creator><creator>Bendinelli, M.</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>199505</creationdate><title>FIV infection of macrophages: in vitro and in vivo inhibition by dideoxycytidine 5′-triphosphate</title><author>Magnani, M. ; Rossi, L. ; Fraternale, A. ; Silvotti, L. ; Quintavalla, F. ; Piedimonte, G. ; Matteucci, D. ; Baldinotti, F. ; Bendinelli, M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c322t-9fedb5a5a8e9855b4c0e93cd09a30748e6d55929fad269c2402b742ac78b212c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>AIDS/HIV</topic><topic>ANIMAL VIRUSES</topic><topic>Animals</topic><topic>ANTIVIRAL</topic><topic>ANTIVIRAL AGENTS</topic><topic>Antiviral Agents - administration & dosage</topic><topic>Antiviral Agents - pharmacology</topic><topic>CATS</topic><topic>CHAT</topic><topic>DEFICIENCE IMMUNOLOGIQUE</topic><topic>DEFICIENCIA INMUNOLOGICA</topic><topic>Deoxycytosine Nucleotides - administration & dosage</topic><topic>Deoxycytosine Nucleotides - pharmacology</topic><topic>Dideoxynucleotides</topic><topic>Drug Carriers</topic><topic>ERITROCITOS</topic><topic>ERYTHROCYTE</topic><topic>ERYTHROCYTES</topic><topic>Feline Acquired Immunodeficiency Syndrome - blood</topic><topic>Feline Acquired Immunodeficiency Syndrome - drug therapy</topic><topic>Feline Acquired Immunodeficiency Syndrome - immunology</topic><topic>feline leukemia virus</topic><topic>Female</topic><topic>GATO</topic><topic>Immunodeficiency Virus, Feline - drug effects</topic><topic>Immunodeficiency Virus, Feline - physiology</topic><topic>IMMUNOLOGICAL DEFICIENCY</topic><topic>Lymphocytes - virology</topic><topic>MACROFAGOS</topic><topic>MACROPHAGE</topic><topic>MACROPHAGES</topic><topic>Macrophages - virology</topic><topic>Macrophages, Peritoneal - virology</topic><topic>Male</topic><topic>Specific Pathogen-Free Organisms</topic><topic>VIRICIDAS</topic><topic>VIRUS DE LOS ANIMALES</topic><topic>VIRUS DES ANIMAUX</topic><topic>Virus Replication - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Magnani, M.</creatorcontrib><creatorcontrib>Rossi, L.</creatorcontrib><creatorcontrib>Fraternale, A.</creatorcontrib><creatorcontrib>Silvotti, L.</creatorcontrib><creatorcontrib>Quintavalla, F.</creatorcontrib><creatorcontrib>Piedimonte, G.</creatorcontrib><creatorcontrib>Matteucci, D.</creatorcontrib><creatorcontrib>Baldinotti, F.</creatorcontrib><creatorcontrib>Bendinelli, M.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary immunology and immunopathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Magnani, M.</au><au>Rossi, L.</au><au>Fraternale, A.</au><au>Silvotti, L.</au><au>Quintavalla, F.</au><au>Piedimonte, G.</au><au>Matteucci, D.</au><au>Baldinotti, F.</au><au>Bendinelli, M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>FIV infection of macrophages: in vitro and in vivo inhibition by dideoxycytidine 5′-triphosphate</atitle><jtitle>Veterinary immunology and immunopathology</jtitle><addtitle>Vet Immunol Immunopathol</addtitle><date>1995-05</date><risdate>1995</risdate><volume>46</volume><issue>1</issue><spage>151</spage><epage>158</epage><pages>151-158</pages><issn>0165-2427</issn><eissn>1873-2534</eissn><abstract>We have evaluated in vitro and in vivo whether it is possible to protect cat macrophages from feline immunodeficiency virus (FIV) infection by the administration of dideoxycytidine 5′-triphosphate (DDCTP). Since cell membranes are impermeable to phosphorylated drugs we have encapsulated DDCTP into autologous erythrocytes and modified erythrocyte membranes to target these drug-loaded cells to macrophages. DDCTP-loaded erythrocytes reduced FIV production by macrophages infected in vitro or obtained from naturally or experimentally infected cats. The same treatment protected the majority of peritoneal macrophages during a 7 month experimental FIV infection and reduced the percentage of circulating lymphocytes stained with an anti-p24 antibody. These results suggest that the administration of nucleoside analogues in phosphorylated form is feasible and their targeting to macrophages reduces FIV infection in vitro and in vivo.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>7618255</pmid><doi>10.1016/0165-2427(94)07014-X</doi><tpages>8</tpages></addata></record> |
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| ispartof | Veterinary immunology and immunopathology, 1995-05, Vol.46 (1), p.151-158 |
| issn | 0165-2427 1873-2534 |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | AIDS/HIV ANIMAL VIRUSES Animals ANTIVIRAL ANTIVIRAL AGENTS Antiviral Agents - administration & dosage Antiviral Agents - pharmacology CATS CHAT DEFICIENCE IMMUNOLOGIQUE DEFICIENCIA INMUNOLOGICA Deoxycytosine Nucleotides - administration & dosage Deoxycytosine Nucleotides - pharmacology Dideoxynucleotides Drug Carriers ERITROCITOS ERYTHROCYTE ERYTHROCYTES Feline Acquired Immunodeficiency Syndrome - blood Feline Acquired Immunodeficiency Syndrome - drug therapy Feline Acquired Immunodeficiency Syndrome - immunology feline leukemia virus Female GATO Immunodeficiency Virus, Feline - drug effects Immunodeficiency Virus, Feline - physiology IMMUNOLOGICAL DEFICIENCY Lymphocytes - virology MACROFAGOS MACROPHAGE MACROPHAGES Macrophages - virology Macrophages, Peritoneal - virology Male Specific Pathogen-Free Organisms VIRICIDAS VIRUS DE LOS ANIMALES VIRUS DES ANIMAUX Virus Replication - drug effects |
| title | FIV infection of macrophages: in vitro and in vivo inhibition by dideoxycytidine 5′-triphosphate |
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